<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Haploid induction using in vitro cultures of unpollinated flowers has been recognized as an important tool to produce homozygous plants for genetic studies and breeding programs. In this study the potential of gynogenic haploid induction in four ecotypes of <italic>Allium hirtifolium</italic> under different combinations of benzylaminopurine (BAP) with 2,4-dichlorophenoxyacetic acid (2,4-D), or α-naphthaleneacetic acid (NAA) was investigated. Unpollinated flower buds were excised from an umbel 5 to 3 days before anthesis, and cultured onto B5 medium containing 7.5% sucrose and 2 mg·dm<sup>−3</sup> BAP with auxin. The experiments revealed that NAA increased the percentage of gynogenesis induction and number of gynogenic embryos per flower in all ecotypes. Somatic organogenesis from basal callus or other floral parts was most effective on the media containing 2,4-D. Plants obtained by gynogenesis were haploid in 70–77% and plants from somatic tissue were mostly diploid.</p></abstract>

Haploid induction using in vitro cultures of unpollinated flowers has been recognized as an important tool to produce homozygous plants for genetic studies and breeding programs. In this study the potential of gynogenic haploid induction in four ecotypes of Allium hirtifolium under different combinations of benzylaminopurine (BAP) with 2,4-dichlorophenoxyacetic acid (2,4-D), or α-naphthaleneacetic acid (NAA) was investigated. Unpollinated flower buds were excised from an umbel 5 to 3 days before anthesis, and cultured onto B5 medium containing 7.5% sucrose and 2 mg·dm−3 BAP with auxin. The experiments revealed that NAA increased the percentage of gynogenesis induction and number of gynogenic embryos per flower in all ecotypes. Somatic organogenesis from basal callus or other floral parts was most effective on the media containing 2,4-D. Plants obtained by gynogenesis were haploid in 70–77% and plants from somatic tissue were mostly diploid.

Haploid Induction via In Vitro Gynogenesis in Persian Shallot (<italic>Allium hirtifolium</italic>)

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Haploid Induction via In Vitro Gynogenesis in Persian Shallot ()

Haploid induction using in vitro cultures of unpollinated flowers has been recognized as an important tool to produce homozygous plants for genetic studies and...

Haploid Induction via In Vitro Gynogenesis in Persian Shallot (Allium hirtifolium)

Published Online: Dec 31, 2019

Page range: 91 - 98

Received: Jun 01, 2019

Accepted: Oct 01, 2019

DOI: https://doi.org/10.2478/johr-2019-0017

Keywords
flower culture, embryogenesis, regeneration from somatic tissues, mixoploids

© 2019 Jaber Panahandeh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Haploid Induction via In Vitro Gynogenesis in Persian Shallot (Allium hirtifolium)

Published Online: Dec 31, 2019

Page range: 91 - 98

Received: Jun 01, 2019

Accepted: Oct 01, 2019

DOI: https://doi.org/10.2478/johr-2019-0017

Keywordsflower culture, embryogenesis, regeneration from somatic tissues, mixoploids

© 2019 Jaber Panahandeh et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
flower culture, embryogenesis, regeneration from somatic tissues, mixoploids