<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Two-spotted spider mite (<italic>Tetranychus urticae</italic>, Koch) possess tremendous ability to develop resistance to pest control chemicals. Hence, although METI-acaricides are quite new group of pesticides, the resistance to this chemicals may shortly become a serious obstacle in two-spotted spider mite management. In presented study, molecular and phenotypic diversity of three laboratory strains and seven <italic>T. urticae</italic> populations collected from pest-protected apple orchards were analysed in reference to METI-acaricides resistance. The inter-population molecular diversity analysis with a set of RAPD markers revealed 6 populations to be in close relation to susceptible reference strain (GSS). Only one population turned out to be linked relatively close with METI-acaricides resistant strain (Akita). Simultaneously, the results of biological test showed similar relation among the analysed populations, according to the resistance incidence ratio. Obtained results suggest that METI-acaricides resistance trait in two-spotted spider mite is likely to be determined at genomic level and may be considered as an initial stage for developing molecular biology tools for early detection of METI-acaricides resistance in <italic>T. urticae</italic> populations.</p></abstract>

Two-spotted spider mite (Tetranychus urticae, Koch) possess tremendous ability to develop resistance to pest control chemicals. Hence, although METI-acaricides are quite new group of pesticides, the resistance to this chemicals may shortly become a serious obstacle in two-spotted spider mite management. In presented study, molecular and phenotypic diversity of three laboratory strains and seven T. urticae populations collected from pest-protected apple orchards were analysed in reference to METI-acaricides resistance. The inter-population molecular diversity analysis with a set of RAPD markers revealed 6 populations to be in close relation to susceptible reference strain (GSS). Only one population turned out to be linked relatively close with METI-acaricides resistant strain (Akita). Simultaneously, the results of biological test showed similar relation among the analysed populations, according to the resistance incidence ratio. Obtained results suggest that METI-acaricides resistance trait in two-spotted spider mite is likely to be determined at genomic level and may be considered as an initial stage for developing molecular biology tools for early detection of METI-acaricides resistance in T. urticae populations.

Molecular Diversity of Two-Spotted Spider Mite (<italic>Tetranychus urticae</italic>) in Apple Orchards Across Poland and its Potential Link with the Resistance to Meti-Acaricides

Faculty of Biology and Environmental Protection

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Molecular Diversity of Two-Spotted Spider Mite () in Apple Orchards Across Poland and its Potential Link with the Resistance to Meti-Acaricides

Two-spotted spider mite (Tetranychus urticae, Koch) possess tremendous ability to develop resistance to pest control chemicals. Hence, although METI-acaricides...

Molecular Diversity of Two-Spotted Spider Mite (Tetranychus urticae) in Apple Orchards Across Poland and its Potential Link with the Resistance to Meti-Acaricides

Published Online: Dec 31, 2018

Page range: 103 - 111

Received: Nov 01, 2018

Accepted: Feb 01, 2019

DOI: https://doi.org/10.2478/johr-2018-0021

Keywords
apple, RAPD polymorphism, acaricides, bioassay

© 2019 Tomasz Jęcz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Molecular Diversity of Two-Spotted Spider Mite (Tetranychus urticae) in Apple Orchards Across Poland and its Potential Link with the Resistance to Meti-Acaricides

Published Online: Dec 31, 2018

Page range: 103 - 111

Received: Nov 01, 2018

Accepted: Feb 01, 2019

DOI: https://doi.org/10.2478/johr-2018-0021

Keywordsapple, RAPD polymorphism, acaricides, bioassay

© 2019 Tomasz Jęcz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
apple, RAPD polymorphism, acaricides, bioassay