<abstract xmlns="http://www.w3.org/1999/xhtml">A simple, accurate and selective HPLC method was developed and validated for determination of quercetin and kaempferol, which are the main flavonols in broccoli. The separation was achieved on a reversed-phase C18 column using a mobile phase composed of methanol/water (60/40) and phosphoric acid 0.2% at a flow rate of 1.0 ml min-1. The detection was carried out on a DAD detector at 370 nm. This method was validated according to the requirements for new methods, which include selectivity, linearity, precision, accuracy, limit of detection and limit of quantitation. The current method demonstrates good linearity, with R2 > 0.99. The recovery is within 98.07-102.15% and 97.92-101.83% for quercetin and kaempferol, respectively. The method is selective, in that quercetin and kaempferol are well separated from other compounds of broccoli with good resolution. The low limit of detection and limit of quantitation of quercetin and kaempferol enable the detection and quantitation of these flavonoids in broccoli at low con–centrations.</abstract>

A simple, accurate and selective HPLC method was developed and validated for determination of quercetin and kaempferol, which are the main flavonols in broccoli. The separation was achieved on a reversed-phase C18 column using a mobile phase composed of methanol/water (60/40) and phosphoric acid 0.2% at a flow rate of 1.0 ml min-1. The detection was carried out on a DAD detector at 370 nm. This method was validated according to the requirements for new methods, which include selectivity, linearity, precision, accuracy, limit of detection and limit of quantitation. The current method demonstrates good linearity, with R2 > 0.99. The recovery is within 98.07-102.15% and 97.92-101.83% for quercetin and kaempferol, respectively. The method is selective, in that quercetin and kaempferol are well separated from other compounds of broccoli with good resolution. The low limit of detection and limit of quantitation of quercetin and kaempferol enable the detection and quantitation of these flavonoids in broccoli at low con–centrations.

Validation of the Analytical Method for the Determination of Flavonoids in Broccoli

Research Institute of Horticulture, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

A simple, accurate and selective HPLC method was developed and validated for determination of quercetin and kaempferol, which are the main flavonols in...

Validation of the Analytical Method for the Determination of Flavonoids in Broccoli

Published Online: Sep 10, 2014

Page range: 131 - 140

Received: Oct 26, 2013

Accepted: May 15, 2014

DOI: https://doi.org/10.2478/johr-2014-0016

Keywords
quercetin, kaempferol, broccoli, validation

© 2014 Magdalena Tuszyńska

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Validation of the Analytical Method for the Determination of Flavonoids in Broccoli

Published Online: Sep 10, 2014

Page range: 131 - 140

Received: Oct 26, 2013

Accepted: May 15, 2014

DOI: https://doi.org/10.2478/johr-2014-0016

Keywordsquercetin, kaempferol, broccoli, validation

© 2014 Magdalena Tuszyńska

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
quercetin, kaempferol, broccoli, validation