<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A one-step RT-PCR procedure was developed for specific detection of Narcissus latent virus (NLV) isolates. Following alignment of RNA sequences of three NLV isolates, the conserved sequence fragments were identified in viral polyprotein gene and 3’UTR region. Based on those fragments, a forward NLs1 and two reverse: NLa1 and NLSCPR2 primers were designed. Primer pairs NLs1-NLa1 and NLs1- NLSCPR2 proved to be effective in amplification of a 669 bp and 1295 bp products, respectively. Sequence analysis of amplified products confirmed their specificity.</p></abstract>

A one-step RT-PCR procedure was developed for specific detection of Narcissus latent virus (NLV) isolates. Following alignment of RNA sequences of three NLV isolates, the conserved sequence fragments were identified in viral polyprotein gene and 3’UTR region. Based on those fragments, a forward NLs1 and two reverse: NLa1 and NLSCPR2 primers were designed. Primer pairs NLs1-NLa1 and NLs1- NLSCPR2 proved to be effective in amplification of a 669 bp and 1295 bp products, respectively. Sequence analysis of amplified products confirmed their specificity.

Detection of Narcissus Latent Virus Isolates Using One-Step Rt-Pcr Assay

Research Institute of Horticulture, Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland

Journal of Horticultural Research

{"article-title":"Detection of Narcissus Latent Virus Isolates Using One-Step Rt-Pcr Assay"}

A one-step RT-PCR procedure was developed for specific detection of Narcissus latent virus (NLV) isolates. Following alignment of RNA sequences of three NLV...

Detection of Narcissus Latent Virus Isolates Using One-Step Rt-Pcr Assay

Published Online: Jul 30, 2013

Page range: 11 - 14

DOI: https://doi.org/10.2478/johr-2013-0002

Keywords
NLV, virus identification, RT-PCR, DAS-ELISA, narcissus cultivars

This content is open access.

Detection of Narcissus Latent Virus Isolates Using One-Step Rt-Pcr Assay

Published Online: Jul 30, 2013

Page range: 11 - 14

DOI: https://doi.org/10.2478/johr-2013-0002

KeywordsNLV, virus identification, RT-PCR, DAS-ELISA, narcissus cultivars

This content is open access.

Keywords
NLV, virus identification, RT-PCR, DAS-ELISA, narcissus cultivars