Reniform Nematode Management Using Winter Crop Rotation and Residue Incorporation Methods in Greenhouse Experiments

Rebeca Sandoval-Ruiz; Zane J. Grabau

Open Access

Reniform Nematode Management Using Winter Crop Rotation and Residue Incorporation Methods in Greenhouse Experiments

Rebeca Sandoval-Ruiz

and

Zane J. Grabau

| Sep 13, 2023

Journal of Nematology

Volume 55 (2023): Issue 1 (February 2023)

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Published Online: Sep 13, 2023

Page range: -

Received: Mar 03, 2023

DOI: https://doi.org/10.2478/jofnem-2023-0035

Keywords
biofumigation, carinata, cotton, hairy vetch, host, management, oat, reniform nematode, rotation, winter crops

© 2023 Rebeca Sandoval-Ruiz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Graphical representation of the methodology to evaluate rotation of winter crops—with varying crop residue incorporation methods—to manage reniform nematode and cotton plant growth.

Reniform nematode (RN) in 100 cm3 of soil at winter crop termination in Trial 1 (A) and Trial 2 (B). Letters indicate treatment separation by Kruskal-Wallis with Bonferroni's method, P < 0.05. Data is displayed as boxplots.

Reniform nematode (RN) in 100 cm3 of soil at cotton harvest, by crop and organic matter incorporation method, in Trial 1 (A) and Trial 2 (B). Ctrl= fallow control, No OM= rotation with no shoot organic matter (OM) incorporated. Fresh OM= treatments with fresh shoot OM, Dry OM= treatments with dry shoot OM. Fresh roots were incorporated in each treatment. Letters indicate treatment separation by Kruskal-Wallis with Bonferroni's method, P < 0.05. Data is displayed as boxplots.

Reniform nematodes (RN) from cotton roots as affected by crop and organic matter incorporation treatments. Reniform nematode abundances are expressed as total RN (eggs plus vermiform nematodes) per gram of cotton roots in Trial 1 (A) and Trial 2 (B) Ctrl= fallow control, No OM= rotation with no shoot organic matter (OM) incorporated. Fresh OM= treatments with fresh shoot OM, Dry OM= treatments with dry shoot OM. Fresh roots were incorporated in each treatment. Letters indicate treatment separation by Kruskal-Wallis with Bonferroni's method, P < 0.05. Data is displayed as boxplots.

Reniform nematode (RN) reproduction factor (total RN per pot final population/inoculated initial population), by crop, at cotton harvest in Trial 1 (A) and Trial 2 (B). Ctrl= fallow control, No OM= rotation with no shoot organic matter (OM) incorporated. Fresh OM= treatments with fresh shoot OM, Dry OM= treatments with dry shoot OM. Fresh roots were incorporated in each treatment. Letters indicate treatment separation by Kruskal-Wallis with Bonferroni's method, P < 0.05. Data is displayed as boxplots.

Winter crop residue measurements by crop rotation and residue incorporation method.

Treatment	Trial 1^a

	Fresh weight (g) of residues added^b	Final OM added (%w/w)^c
p-value	***	***
Rotation+No OM^d
Carinata	20.3 ± 1.6 b	1.3 ± 0.1 bc
Hairy vetch	27.0 ± 5.2 b	1.8 ± 0.3 b
Oat	68.4 ± 10.9 a	4.5 ± 0.7 a
Rotation+Fresh OM
Carinata	37.8 ± 3.0 b	2.5 ± 0.2 b
Hairy vetch	26.0 ± 7.5 b	1.7 ± 0.5 b
Oat	78.5 ± 5.4 a	5.2 ± 0.3 a
Rotation+Dry OM
Carinata	28.4 ± 2.5 b	0.9 ± 0.0 bc
Hairy vetch	26.9 ± 3.4 b	1.4 ± 0.1 bc
Oat	91.1 ± 2.7 a	4.2 ± 0.3 a

Influence of winter crop rotation and residue incorporation method, on cotton growth and SPAD value in greenhouse conditions under the presence of reniform nematode.

Treatment	Canopy cover	Height (cm)	Root fresh weight (g)	Root fresh weight (g)	Shoot fresh weight (g)	SPAD

	Trial 1^a
p-value^b	**	***	***	ns	***	***
Control - (Fallow)	4.0 ± 0.3 abc	10.8 ± 0.4 ab	4.6 ± 0.4 e	1.4 ± 0.1	4.7 ± 0.3 ab	46.1 ± 0.9 a
Rotation+No OM^c
Carinata	5.2 ± 0.3 a	12.0 ± 0.5 a	13.3 ± 1.0 abc	1.3 ± 0	5.3 ± 0.3 a	38.8 ± 1.3 bc
Hairy vetch	4.0 ± 0.3 abc	10.7 ± 0.3 ab	11.1 ± 0.9 bcd	1.0 ± 0	4.3 ± 0.3 ab	36.6 ± 1.1 c
Oat	4.0 ± 0.5 abc	10.4 ± 0.6 ab	20.5 ± 2.7 a	1.0 ± 0	3.8 ± 0.3 ab	44.8 ± 1.2 ab
Rotation+Fresh OM
Carinata	4.9 ± 0.4 ab	11.8 ± 0.5 a	11.0 ± 1.9 bcd	1.3 ± 0.1	5.3 ± 0.4 a	41.6 ± 0.7 abc
Hairy vetch	3.9 ± 0.5 abc	11.0 ± 0.7 ab	6.3 ± 0.7 de	1.1 ± 0.2	4.1 ± 0.6 ab	38.2 ± 1.6 bc
Oat	2.9 ± 0.2 c	9.2 ± 0.5 b	14.5 ± 2.1 ab	0.9 ± 0.0	3.3 ± 0.2 b	42.1 ± 1.3 abc
Rotation+Dry OM
Carinata	3.8 ± 0.3 abc	10.0 ± 0.4 ab	7.4 ± 0.6 cde	1.0 ± 0.0	4.0 ± 0.2 ab	40.5 ± 1.4 abc
Hairy vetch	3.9 ± 0.4 abc	10.4 ± 0.6 ab	4.7 ± 0.8 e	0.9 ± 0.0	3.8 ± 0.3 ab	37.9 ± 1.7 c
Oat	3.2 ± 0.3 bc	9.1 ± 0.3 b	17.2 ± 2.2 ab	0.9 ± 0.0	3.2 ± 0.2 b	41.1 ±1.9 abc

Trial 2^a

p-value^b	ns	ns	***	ns	ns	ns
Control - (Fallow)	5.5 ± 0.5	12.0 ± 0.6	4.0 ± 0.3 b	1.6 ± 0.1	5.2 ± 0.4	56.2 ± 1.3
Rotation+No OM
Carinata	8.2 ± 1.7	14.0 ± 1.1	6.5 ± 0.6 ab	1.9 ± 0.1	6.8 ± 0.6	49.9 ± 0.7
Hairy vetch	8.9 ± 2.7	16.5 ± 2.1	8.3 ± 1.4 a	2.1 ± 0.3	6.8 ±1.2	47.8 ± 3.4
Oat	6.9 ± 0.8	13.5 ± 0.8	6.1 ± 0.6 ab	1.7 ± 0.1	5.4 ± 0.4	49.6 ± 1.5
Rotation+Fresh OM
Carinata	9.8 ± 1.7	15.4 ± 1.2	8.5 ± 0.8 a	2.4 ± 0.2	7.9 ± 0.8	49.2 ± 1.6
Hairy vetch	6.3 ± 1.2	13.1 ± 1.7	6.1 ± 1.3 ab	1.8 ± 0.3	5.9 ± 1.3	48.7 ± 2.0
Oat	7.4 ± 0.9	14 ± 0.8	5.4 ± 1.0 ab	2.0 ± 0.3	6.3 ± 0.8	52.4 ± 1.7
Rotation+Dry OM
Carinata	7.4 ± 1.6	14.6 ± 1.2	4.4 ± 1.0 b	1.8 ± 0.2	5.9 ± 0.7	49.2 ± 1.3
Hairy vetch	7.2 ± 0.7	14.3 ± 0.5	6.0 ± 0.6 ab	2.2 ± 0.1	6.7 ± 0.5	48.1 ± 1.8
Oat	6.8 ± 0.6	14.0 ± 0.7	4.8 ± 0.2 ab	1.6 ± 0	5.4 ± 0.3	53.4 ± 1.4

Schedule for plant data collection and management in greenhouse trials.

	Trial 1		Trial 2

	Date	DAP^a	Date	DAP^a
1. Winter Crops
1.1 Planting and nematode inoculation	12 November 2021	0	29 December 2022	0
1.2 Harvest
• Dry OM treatments	25 January 2022	74	14 March 2022	75
• No OM treatments	27 January 2022	76	16 March 2022	76
• Fresh OM treatments	27 January 2022	76	16 March 2022	76
1.3 OM incorporation	28 January 2022	77	17 March 2022	78
2. Cotton
2.1 Planting	8 February 2022	0	28 March 2022	0
2.2 Plant growth assessment	6 April 2022	57	24 May 2022	57
2.2 Harvest	10 April 2022	61	27 May 2022	60

eISSN:: 2640-396X
Language:: English

Publication timeframe:: Volume Open
Journal Subjects:: Life Sciences, other

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Reniform Nematode Management Using Winter Crop Rotation and Residue Incorporation Methods in Greenhouse Experiments

Article Category: Research Paper

Published Online: Sep 13, 2023

Page range: -

Received: Mar 03, 2023

DOI: https://doi.org/10.2478/jofnem-2023-0035

Keywords
biofumigation, carinata, cotton, hairy vetch, host, management, oat, reniform nematode, rotation, winter crops

© 2023 Rebeca Sandoval-Ruiz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Figure 2:

Figure 3:

Figure 4:

Figure 5:

Winter crop residue measurements by crop rotation and residue incorporation method.

Influence of winter crop rotation and residue incorporation method, on cotton growth and SPAD value in greenhouse conditions under the presence of reniform nematode.

Schedule for plant data collection and management in greenhouse trials.

Reniform Nematode Management Using Winter Crop Rotation and Residue Incorporation Methods in Greenhouse Experiments

Article Category: Research Paper

Published Online: Sep 13, 2023

Page range: -

Received: Mar 03, 2023

DOI: https://doi.org/10.2478/jofnem-2023-0035

Keywordsbiofumigation, carinata, cotton, hairy vetch, host, management, oat, reniform nematode, rotation, winter crops

© 2023 Rebeca Sandoval-Ruiz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Figure 2:

Figure 3:

Figure 4:

Figure 5:

Winter crop residue measurements by crop rotation and residue incorporation method.

Influence of winter crop rotation and residue incorporation method, on cotton growth and SPAD value in greenhouse conditions under the presence of reniform nematode.

Schedule for plant data collection and management in greenhouse trials.

Keywords
biofumigation, carinata, cotton, hairy vetch, host, management, oat, reniform nematode, rotation, winter crops