<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>We previously reported soybean fields double-cropped with winter wheat having reduced soybean cyst nematode (SCN) (<italic>Heterodera glycines</italic>) counts compared to fallow. A follow-up metagenomics study identified several fungal and bacterial taxa enriched in wheat fields, and some were reported to parasitize SCN. Knowing that phytocompounds with potential nematicidal activity are released via wheat roots and stubble, we implemented a dichloromethane-based extraction method and a gas chromatography-mass spectrometry (GCMS) system to investigate soil chemical profiles of samples collected from these fields and review the potential nematicidal activity of compounds with higher concentration in double cropping fields. 51 compounds were detected during the GCMS analysis, eight with unknown identification. Several compounds, including multiple fatty acids, had larger relative peak areas when double-cropped, compared to fallow samples. This study, along with our previously published one, provided a better understanding of the mechanisms that govern the effect of wheat on SCN populations. Rather than driven by a single mechanism, the suppression of SCN in soybean fields double-cropped with winter wheat was potentially linked to enriched microbial communities, increased populations of beneficial organisms, and higher concentrations of chemicals with potential nematicidal activity. To our knowledge, this is the first study using GCMS to characterize soil chemical profiles in soybean fields double-cropped with winter wheat regarding the suppression of SCN populations.</p>
</abstract>

We previously reported soybean fields double-cropped with winter wheat having reduced soybean cyst nematode (SCN) (Heterodera glycines) counts compared to fallow. A follow-up metagenomics study identified several fungal and bacterial taxa enriched in wheat fields, and some were reported to parasitize SCN. Knowing that phytocompounds with potential nematicidal activity are released via wheat roots and stubble, we implemented a dichloromethane-based extraction method and a gas chromatography-mass spectrometry (GCMS) system to investigate soil chemical profiles of samples collected from these fields and review the potential nematicidal activity of compounds with higher concentration in double cropping fields. 51 compounds were detected during the GCMS analysis, eight with unknown identification. Several compounds, including multiple fatty acids, had larger relative peak areas when double-cropped, compared to fallow samples. This study, along with our previously published one, provided a better understanding of the mechanisms that govern the effect of wheat on SCN populations. Rather than driven by a single mechanism, the suppression of SCN in soybean fields double-cropped with winter wheat was potentially linked to enriched microbial communities, increased populations of beneficial organisms, and higher concentrations of chemicals with potential nematicidal activity. To our knowledge, this is the first study using GCMS to characterize soil chemical profiles in soybean fields double-cropped with winter wheat regarding the suppression of SCN populations.

Chemical Profiles of <italic>Heterodera glycines</italic> Suppressive Soils in Double Cropping Soybean Production

School of Chemical and Biomolecular Sciences

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Chemical Profiles of  Suppressive Soils in Double Cropping Soybean Production

We previously reported soybean fields double-cropped with winter wheat having reduced soybean cyst nematode (SCN) (Heterodera glycines) counts compared to...

Chemical Profiles of Heterodera glycines Suppressive Soils in Double Cropping Soybean Production

Article Category: Research Paper

Published Online: Aug 29, 2023

Page range: -

Received: Jan 04, 2023

DOI: https://doi.org/10.2478/jofnem-2023-0030

Keywords
gas chromatography-mass spectrometry (GCMS), host-parasitic relationship, management, metabolomics, suppressive soil

© 2023 Leonardo F. Rocha et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Chemical Profiles of Heterodera glycines Suppressive Soils in Double Cropping Soybean Production

Article Category: Research Paper

Published Online: Aug 29, 2023

Page range: -

Received: Jan 04, 2023

DOI: https://doi.org/10.2478/jofnem-2023-0030

Keywordsgas chromatography-mass spectrometry (GCMS), host-parasitic relationship, management, metabolomics, suppressive soil

© 2023 Leonardo F. Rocha et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
gas chromatography-mass spectrometry (GCMS), host-parasitic relationship, management, metabolomics, suppressive soil