<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>In this paper, the possibility of using cell culture impedance measurements to assess the biocompatibility of a material in contact with cells was analyzed. For this purpose, the Electric Cell-substrate Impedance Sensing (ECIS) method and a commercial measuring device were used. The test substrates with thin-film electrodes made of various metals were prepared using the magnetron sputtering method. The choice of metals was dictated by their varying degrees of biocompatibility. Cultures of mouse fibroblasts were cultured on the prepared substrates. The experiment showed that the complete cycle of culture from attachment and reproduction to apoptosis occurred. The results obtained indicate that it is possible to use the ECIS method to study the influence of metal on cell culture activity.</p></abstract>

In this paper, the possibility of using cell culture impedance measurements to assess the biocompatibility of a material in contact with cells was analyzed. For this purpose, the Electric Cell-substrate Impedance Sensing (ECIS) method and a commercial measuring device were used. The test substrates with thin-film electrodes made of various metals were prepared using the magnetron sputtering method. The choice of metals was dictated by their varying degrees of biocompatibility. Cultures of mouse fibroblasts were cultured on the prepared substrates. The experiment showed that the complete cycle of culture from attachment and reproduction to apoptosis occurred. The results obtained indicate that it is possible to use the ECIS method to study the influence of metal on cell culture activity.

Electric cell-substrate impedance sensing in biocompatibility research

Journal of Electrical Bioimpedance

This work is licensed under the Creative Commons Attribution 4.0 International License.

{"article-title":"Electric cell-substrate impedance sensing in biocompatibility research"}

In this paper, the possibility of using cell culture impedance measurements to assess the biocompatibility of a material in contact with cells was analyzed....

Electric cell-substrate impedance sensing in biocompatibility research

Published Online: Dec 30, 2021

Page range: 163 - 168

Received: Nov 30, 2021

DOI: https://doi.org/10.2478/joeb-2021-0019

Keywords
Bioimpedance, biocompatibility, cell culture, technology

© 2021 Andrzej Kociubiński, published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Electric cell-substrate impedance sensing in biocompatibility research

Published Online: Dec 30, 2021

Page range: 163 - 168

Received: Nov 30, 2021

DOI: https://doi.org/10.2478/joeb-2021-0019

KeywordsBioimpedance, biocompatibility, cell culture, technology

© 2021 Andrzej Kociubiński, published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
Bioimpedance, biocompatibility, cell culture, technology