<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The study aimed to investigate glutathione peroxidase (GPx) activity and glutathione (GSH) levels in the sperm of patients with unexplained infertility. The sperm samples were collected from subjects with normal semen parameters divided into fertile and infertile groups. Sperm analysis was performed according to the 2010 WHO criteria. Measurement of the GPx activity and GSH were performed by enzymatic assay kits. The higher enzymatic activity recorded in spermatozoa and seminal plasma in the infertile group was close to the significant one – p=0.054 for seminal plasma andp= 0.086 for the spermatozoa.GSH levels were higher in the fertile group in the seminal plasma (p=0.045). Defining the causes of unexplained infertility requires the addition of oxidative stress. In patients with unexplained infertility, the level of glutathione is reduced, and the activity of one of the significant enzyme antioxidants GPx is not changed significantly and even shows a tendency to rise.</p>
</abstract>

The study aimed to investigate glutathione peroxidase (GPx) activity and glutathione (GSH) levels in the sperm of patients with unexplained infertility. The sperm samples were collected from subjects with normal semen parameters divided into fertile and infertile groups. Sperm analysis was performed according to the 2010 WHO criteria. Measurement of the GPx activity and GSH were performed by enzymatic assay kits. The higher enzymatic activity recorded in spermatozoa and seminal plasma in the infertile group was close to the significant one – p=0.054 for seminal plasma andp= 0.086 for the spermatozoa.GSH levels were higher in the fertile group in the seminal plasma (p=0.045). Defining the causes of unexplained infertility requires the addition of oxidative stress. In patients with unexplained infertility, the level of glutathione is reduced, and the activity of one of the significant enzyme antioxidants GPx is not changed significantly and even shows a tendency to rise.

Glutathione Content and Glutathione Peroxidase Activity of Sperm in Males with Unexplained Infertility

Department of Anatomy, Histology, Cytology and Biology

Journal of Biomedical and Clinical Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The study aimed to investigate glutathione peroxidase (GPx) activity and glutathione (GSH) levels in the sperm of patients with unexplained infertility. The...

Glutathione Content and Glutathione Peroxidase Activity of Sperm in Males with Unexplained Infertility

Published Online: Jul 12, 2021

Page range: 53 - 61

Received: Dec 04, 2020

Accepted: May 27, 2021

DOI: https://doi.org/10.2478/jbcr-2021-0006

Keywords
male infertility, oxidative stress, glutathione peroxidase, glutathione

© 2021 Danail I. Martinov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Glutathione Content and Glutathione Peroxidase Activity of Sperm in Males with Unexplained Infertility

Published Online: Jul 12, 2021

Page range: 53 - 61

Received: Dec 04, 2020

Accepted: May 27, 2021

DOI: https://doi.org/10.2478/jbcr-2021-0006

Keywordsmale infertility, oxidative stress, glutathione peroxidase, glutathione

© 2021 Danail I. Martinov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
male infertility, oxidative stress, glutathione peroxidase, glutathione