<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Surveys were conducted in Malatya and Elazığ provinces of Eastern Türkiye between 2018–2019 to detect the presence and distribution of <italic>Deformed wing virus</italic> (DWV), <italic>Acute bee paralysis virus</italic> (ABPV) and <italic>Black queen cell virus</italic> (BQCV) infecting honey bees and <italic>Varroa</italic> mites. Thirty <italic>Varroa</italic> mites feeding on honey bees and 147 honey bees were collected from twenty-five apiaries of Malatya and fifteen apiaries of Elazığ. The collected samples were subjected to reverse transcriptase-polymerase chain reaction. All tested viruses were found to be present in the samples singly or as mixed infections. The prevalence of the viruses were 23.81%, 12.93%, and 10.20% for DWV, ABPV and BQCV, respectively. Five out of thirty <italic>Varroa</italic> mites tested for the viruses were found to be infected only with DWV. Randomly chosen sequences of each detected virus’s partial polyprotein gene region were registered in GenBank under the accession numbers OP805878, OP805879, OP805880, OP805887, OP805888, OP805889, OP805890, OP805891 (DWV), OP805881, OP805882, OP805883, OP805884 (ABPV) and OP805885, OP805886 (BQCV). The phylogenetic tree of the viral isolates were compared with the world isolates. DWV isolates were found to be closely related to the UK, Lebanon, Türkiye, France, Germany and Israel isolates, whereas ABPV isolates were related to Türkiye, South Africa, Slovenia, Serbia, France, Hungary, Syria and USA isolates and BQCV isolates were related to China, South Korea, Czech Republic, France, Hungary, Poland, South Africa, Australia and Sweden isolates. The presence of DWV, ABPV and BQCV infections in Malatya and Elazığ provinces of Türkiye is revealed for the first time in this study.</p>
</abstract>

Surveys were conducted in Malatya and Elazığ provinces of Eastern Türkiye between 2018–2019 to detect the presence and distribution of Deformed wing virus (DWV), Acute bee paralysis virus (ABPV) and Black queen cell virus (BQCV) infecting honey bees and Varroa mites. Thirty Varroa mites feeding on honey bees and 147 honey bees were collected from twenty-five apiaries of Malatya and fifteen apiaries of Elazığ. The collected samples were subjected to reverse transcriptase-polymerase chain reaction. All tested viruses were found to be present in the samples singly or as mixed infections. The prevalence of the viruses were 23.81%, 12.93%, and 10.20% for DWV, ABPV and BQCV, respectively. Five out of thirty Varroa mites tested for the viruses were found to be infected only with DWV. Randomly chosen sequences of each detected virus’s partial polyprotein gene region were registered in GenBank under the accession numbers OP805878, OP805879, OP805880, OP805887, OP805888, OP805889, OP805890, OP805891 (DWV), OP805881, OP805882, OP805883, OP805884 (ABPV) and OP805885, OP805886 (BQCV). The phylogenetic tree of the viral isolates were compared with the world isolates. DWV isolates were found to be closely related to the UK, Lebanon, Türkiye, France, Germany and Israel isolates, whereas ABPV isolates were related to Türkiye, South Africa, Slovenia, Serbia, France, Hungary, Syria and USA isolates and BQCV isolates were related to China, South Korea, Czech Republic, France, Hungary, Poland, South Africa, Australia and Sweden isolates. The presence of DWV, ABPV and BQCV infections in Malatya and Elazığ provinces of Türkiye is revealed for the first time in this study.

Prevalence and Molecular Characterization of Deformed Wing, Acute Bee Paralysis and Black Queen Cell Viruses Infecting Honey Bees and <italic>Varroa</italic> Mites

Agricultural Faculty, Plant Protection Department

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Prevalence and Molecular Characterization of Deformed Wing, Acute Bee Paralysis and Black Queen Cell Viruses Infecting Honey Bees and  Mites

Surveys were conducted in Malatya and Elazığ provinces of Eastern Türkiye between 2018–2019 to detect the presence and distribution of Deformed wing virus...

Prevalence and Molecular Characterization of Deformed Wing, Acute Bee Paralysis and Black Queen Cell Viruses Infecting Honey Bees and Varroa Mites

Article Category: Original Article

Published Online: Jun 30, 2023

Page range: 73 - 86

Received: Feb 24, 2023

Accepted: May 18, 2023

DOI: https://doi.org/10.2478/jas-2023-0006

Keywords
ABPV, BQCV, DWV, honey bee, Türkiye,

© 2023 Songül Aydın et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Prevalence and Molecular Characterization of Deformed Wing, Acute Bee Paralysis and Black Queen Cell Viruses Infecting Honey Bees and Varroa Mites

Article Category: Original Article

Published Online: Jun 30, 2023

Page range: 73 - 86

Received: Feb 24, 2023

Accepted: May 18, 2023

DOI: https://doi.org/10.2478/jas-2023-0006

KeywordsABPV, BQCV, DWV, honey bee, Türkiye,

© 2023 Songül Aydın et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
ABPV, BQCV, DWV, honey bee, Türkiye,