<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Toxic honeydew produced by Flatidae <italic>Epormenis cestri</italic> in Uruguay has been shown to cause among honeybees (<italic>Apis mellifera</italic>) colonies a massive larva death called “River disease”, but the intrinsic mechanisms are still unknown. Because fat body cells, oenocytes and trophocytes, are known to regulated larvae metabolism, and to be affected by xenobiotics, we tested whether apoptosis of these cells can be an underlying cause of larvae death. Ten colonies were divided into two groups and fed with common honey or toxic honeydew obtained from colonies affected by “River disease”. Five-dayold larvae were collected and processed for histology and immunohistochemistry for caspase-3. The area, diameter, and immunostaining area in oenocytes and trophocytes were measured. The oenocyte and trophocyte cellular area decreased in the treated group (p=0.002; p&lt;0.001 respectively) compared to the control group. The diameter of oenocytes (p=0.0002) and trophocytes (p&lt;0.0001) decreased in the treated group. Caspase-3 was detected in cytoplasm in the control group but in the cytoplasm and nucleus in the treated group. The caspase-3 immunostaining area increased in oenocytes (p&lt;0.002) and trophocytes (p&lt;0.0001) of the treated group. The ingestion of toxic honeydew altered the morphology, localization and immunoexpression of caspase-3 in fat body cells, which suggests that the deregulation of the apoptotic mechanism affected the normal development in <italic>A. mellifera</italic> larvae.</p>
</abstract>

Toxic honeydew produced by Flatidae Epormenis cestri in Uruguay has been shown to cause among honeybees (Apis mellifera) colonies a massive larva death called “River disease”, but the intrinsic mechanisms are still unknown. Because fat body cells, oenocytes and trophocytes, are known to regulated larvae metabolism, and to be affected by xenobiotics, we tested whether apoptosis of these cells can be an underlying cause of larvae death. Ten colonies were divided into two groups and fed with common honey or toxic honeydew obtained from colonies affected by “River disease”. Five-dayold larvae were collected and processed for histology and immunohistochemistry for caspase-3. The area, diameter, and immunostaining area in oenocytes and trophocytes were measured. The oenocyte and trophocyte cellular area decreased in the treated group (p=0.002; p<0.001 respectively) compared to the control group. The diameter of oenocytes (p=0.0002) and trophocytes (p<0.0001) decreased in the treated group. Caspase-3 was detected in cytoplasm in the control group but in the cytoplasm and nucleus in the treated group. The caspase-3 immunostaining area increased in oenocytes (p<0.002) and trophocytes (p<0.0001) of the treated group. The ingestion of toxic honeydew altered the morphology, localization and immunoexpression of caspase-3 in fat body cells, which suggests that the deregulation of the apoptotic mechanism affected the normal development in A. mellifera larvae.

Increased Caspase-3 Immunoexpression and Morphology Alterations in Oenocytes and Trophocytes of <italic>Apis mellifera</italic> Larvae Induced by Toxic Secretion of <italic>Epormenis cestri</italic>

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Increased Caspase-3 Immunoexpression and Morphology Alterations in Oenocytes and Trophocytes of  Larvae Induced by Toxic Secretion of 

Toxic honeydew produced by Flatidae Epormenis cestri in Uruguay has been shown to cause among honeybees (Apis mellifera) colonies a massive larva death called...

Increased Caspase-3 Immunoexpression and Morphology Alterations in Oenocytes and Trophocytes of Apis mellifera Larvae Induced by Toxic Secretion of Epormenis cestri

Article Category: Original Article

Published Online: Apr 26, 2021

Page range: 101 - 108

Received: Jan 30, 2020

Accepted: Nov 15, 2020

DOI: https://doi.org/10.2478/jas-2021-0006

Keywords
caspase-3, honeybee larvae, oenocytes, trophocytes, River disease

© 2021 María H. Viotti et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Increased Caspase-3 Immunoexpression and Morphology Alterations in Oenocytes and Trophocytes of Apis mellifera Larvae Induced by Toxic Secretion of Epormenis cestri

Article Category: Original Article

Published Online: Apr 26, 2021

Page range: 101 - 108

Received: Jan 30, 2020

Accepted: Nov 15, 2020

DOI: https://doi.org/10.2478/jas-2021-0006

Keywordscaspase-3, honeybee larvae, oenocytes, trophocytes, River disease

© 2021 María H. Viotti et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
caspase-3, honeybee larvae, oenocytes, trophocytes, River disease