<abstract xmlns="http://www.w3.org/1999/xhtml">

Bee pollen, one of the economic bee products, is widely known as a nutritional food with many bioactivities that depend on many such factors as bee species, plant source and biogeography. For this study, bee pollen was collected from <italic>Apis mellifera</italic>, harvested from the flowers of mimosa (<italic>Mimosa pigra</italic> L.) in the Chiang Mai province, Thailand. The sample was extracted in methanol (MeOH) and then sequentially partitioned with hexane, dichloromethane (DCM) and MeOH in order to isolate compounds depending on their polarities. The obtained extracts were then tested for their antioxidant and anti-tyrosinase activities through 1,1-diphyenyl-2-picrylhydrazyl (DPPH) assay and for/through inhibition of mushroom tyrosinase extract, respectively. The DCM partitioned extract of mimosa flower bee pollen (DCMMBP) provided the highest antioxidant activity, with an effective concentration at 50% (EC50) of 192.1 μg/mL, and was further fractionated by silica gel 60 column chromatography and Sephadex LH20 size exclusion chromatography. All fractions were tested for their antioxidant activity and analyzed for the chemical structure through nuclear magnetic resonance (NMR). The most active fraction (EC50 of 121.3 μg/mL) was a mixture of compounds, but the isolated flavonoid, naringenin, had a negligible antioxidant activity. In contrast, all three partitioned extracts and pooled fractions after silica gel 60 column chromatography provided no anti-tyrosinase activity (IC50 of > 500 μg/mL) and a very low percentage of tyrosinase inhibition (0–2.99 ± 3.18%), compared to kojic acid (IC50 of 8.58 μg/mL and tyrosinase inhibition of 74.2 ± 1.25%).
</abstract>

Bee pollen, one of the economic bee products, is widely known as a nutritional food with many bioactivities that depend on many such factors as bee species, plant source and biogeography. For this study, bee pollen was collected from Apis mellifera, harvested from the flowers of mimosa (Mimosa pigra L.) in the Chiang Mai province, Thailand. The sample was extracted in methanol (MeOH) and then sequentially partitioned with hexane, dichloromethane (DCM) and MeOH in order to isolate compounds depending on their polarities. The obtained extracts were then tested for their antioxidant and anti-tyrosinase activities through 1,1-diphyenyl-2-picrylhydrazyl (DPPH) assay and for/through inhibition of mushroom tyrosinase extract, respectively. The DCM partitioned extract of mimosa flower bee pollen (DCMMBP) provided the highest antioxidant activity, with an effective concentration at 50% (EC50) of 192.1 μg/mL, and was further fractionated by silica gel 60 column chromatography and Sephadex LH20 size exclusion chromatography. All fractions were tested for their antioxidant activity and analyzed for the chemical structure through nuclear magnetic resonance (NMR). The most active fraction (EC50 of 121.3 μg/mL) was a mixture of compounds, but the isolated flavonoid, naringenin, had a negligible antioxidant activity. In contrast, all three partitioned extracts and pooled fractions after silica gel 60 column chromatography provided no anti-tyrosinase activity (IC50 of > 500 μg/mL) and a very low percentage of tyrosinase inhibition (0–2.99 ± 3.18%), compared to kojic acid (IC50 of 8.58 μg/mL and tyrosinase inhibition of 74.2 ± 1.25%).

Screening and Bioguided Fractionation of <italic>Mimosa pigra</italic> L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities

Program in Biotechnology, Faculty of Science

Department of Chemistry, Faculty of Science and Technology

Division of Exploration and Synthesis of Bioactive Compounds, Research Center for Bio-Molecule Engineering

Department of Chemistry, Faculty of Science

Department of Biology, Faculty of Science

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Screening and Bioguided Fractionation of  L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities

Bee pollen, one of the economic bee products, is widely known as a nutritional food with many bioactivities that depend on many such factors as bee species,...

Screening and Bioguided Fractionation of Mimosa pigra L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities

Article Category: Original Article

Published Online: Mar 03, 2021

Page range: 71 - 83

Received: Jun 11, 2019

Accepted: Nov 09, 2020

DOI: https://doi.org/10.2478/jas-2021-0001

Keywords
bee pollen, chromatography, mimosa, naringenin, partitioned extract

© 2021 Phanthiwa Khongkarat et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Screening and Bioguided Fractionation of Mimosa pigra L. Bee Pollen with Antioxidant and Anti-Tyrosinase Activities

Article Category: Original Article

Published Online: Mar 03, 2021

Page range: 71 - 83

Received: Jun 11, 2019

Accepted: Nov 09, 2020

DOI: https://doi.org/10.2478/jas-2021-0001

Keywordsbee pollen, chromatography, mimosa, naringenin, partitioned extract

© 2021 Phanthiwa Khongkarat et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
bee pollen, chromatography, mimosa, naringenin, partitioned extract