<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Propolis is produced by the honeybees by using resin and other plant secretions. Propolis from different geographical regions have different chemical compositions. This is because the chemical constituents of propolis depend on the vegetation surrounding the apiary. In this report we present a new approach using DNA barcoding for the identification of the botanical origin of propolis. Red propolis samples were collected at different times of the year from the state of Sergipe situated in Northeast Brazil. Extraction of the DNA from propolis was made using a CTA B method. Amplification was done using ITS 2 universal primers, followed by DNA sequencing. Sequence analysis confirmed the presence of Dalbergia ecastaphyllum in the Brazilian red propolis. Formononetin is a chemical marker for the Brazillian red propolis and D. ecastaphyllum. Propolis samples analysed by DNA sequencing, were also checked by Ultra-Fast Liquid Chromatography for the presence of formononetin. Peaks corresponding to formononetin were observed in all the analysed propolis samples. This is the first report of the botanical origin of propolis using DNA technology.</p></abstract>

Propolis is produced by the honeybees by using resin and other plant secretions. Propolis from different geographical regions have different chemical compositions. This is because the chemical constituents of propolis depend on the vegetation surrounding the apiary. In this report we present a new approach using DNA barcoding for the identification of the botanical origin of propolis. Red propolis samples were collected at different times of the year from the state of Sergipe situated in Northeast Brazil. Extraction of the DNA from propolis was made using a CTA B method. Amplification was done using ITS 2 universal primers, followed by DNA sequencing. Sequence analysis confirmed the presence of Dalbergia ecastaphyllum in the Brazilian red propolis. Formononetin is a chemical marker for the Brazillian red propolis and D. ecastaphyllum. Propolis samples analysed by DNA sequencing, were also checked by Ultra-Fast Liquid Chromatography for the presence of formononetin. Peaks corresponding to formononetin were observed in all the analysed propolis samples. This is the first report of the botanical origin of propolis using DNA technology.

Botanical Origin of the Brazilian Red Propolis: a New Approach Using DNA analysis

Department of Biology, Federal University of Sergipe Av. Marechal Rondon, s/n Jardim Rosa Elze, 49100-000 São Cristóvão (Sergipe), Brazil

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Propolis is produced by the honeybees by using resin and other plant secretions. Propolis from different geographical regions have different chemical...

Botanical Origin of the Brazilian Red Propolis: a New Approach Using DNA analysis

Published Online: Dec 11, 2014

Page range: 79 - 85

Received: Aug 19, 2014

Accepted: Nov 12, 2014

DOI: https://doi.org/10.2478/jas-2014-0024

Keywords
Apis mellifera, Brazilian red propolis, DNA, formononetin, molecular approach, plant source

© by Sona Jain

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Botanical Origin of the Brazilian Red Propolis: a New Approach Using DNA analysis

Published Online: Dec 11, 2014

Page range: 79 - 85

Received: Aug 19, 2014

Accepted: Nov 12, 2014

DOI: https://doi.org/10.2478/jas-2014-0024

KeywordsApis mellifera, Brazilian red propolis, DNA, formononetin, molecular approach, plant source

© by Sona Jain

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Apis mellifera, Brazilian red propolis, DNA, formononetin, molecular approach, plant source