<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Nanoparticles are utilized in a wide range of industries. The most studied silver nanoparticles (AgNPs) are used in medicine and also in several wound dressings due to their antimicrobial properties. The inflammatory response or potential morphological changes of skin cells after their application are not well known yet. In our study we used the model of human reconstructed epidermis (RHE), prepared in our laboratory, to evaluate whether the AgNPs penetrate through RHE, induce some morphological changes of keratinocytes or influence the production of pro-inflammatory cytokines (IL-6 and IL-8). After the application of three different concentrations (25 ppm, 2.5 ppm, 0.25 ppm) of AgNPs to of RHE for 24 hours we verified that AgNPs did not affect the production of pro-inflammatory cytokines (IL-6 and IL-8) and neither did they influence the expression of keratin K14 and loricrin. The morphology of the cells was likewise unchanged. Based on these results we conclude that AgNPs do not have any negative effect on the morphological changes and do not increase the production of pro-inflammatory cytokines.</p></abstract>

Nanoparticles are utilized in a wide range of industries. The most studied silver nanoparticles (AgNPs) are used in medicine and also in several wound dressings due to their antimicrobial properties. The inflammatory response or potential morphological changes of skin cells after their application are not well known yet. In our study we used the model of human reconstructed epidermis (RHE), prepared in our laboratory, to evaluate whether the AgNPs penetrate through RHE, induce some morphological changes of keratinocytes or influence the production of pro-inflammatory cytokines (IL-6 and IL-8). After the application of three different concentrations (25 ppm, 2.5 ppm, 0.25 ppm) of AgNPs to of RHE for 24 hours we verified that AgNPs did not affect the production of pro-inflammatory cytokines (IL-6 and IL-8) and neither did they influence the expression of keratin K14 and loricrin. The morphology of the cells was likewise unchanged. Based on these results we conclude that AgNPs do not have any negative effect on the morphological changes and do not increase the production of pro-inflammatory cytokines.

Effect of AgNPs on the human reconstructed epidermis

Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry

Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry

Department of Histology and Embryology, Faculty of Medicine and Dentistry

Department of Plastic and Aesthetic Surgery

Interdisciplinary Toxicology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Effect of AgNPs on the human reconstructed epidermis"}

Nanoparticles are utilized in a wide range of industries. The most studied silver nanoparticles (AgNPs) are used in medicine and also in several wound...

Effect of AgNPs on the human reconstructed epidermis

Published Online: Oct 18, 2019

Page range: 289 - 293

Received: Nov 21, 2017

Accepted: Feb 23, 2018

DOI: https://doi.org/10.2478/intox-2018-0028

Keywords
human reconstructed epidermis, AgNPs, IL-6, IL-8, loricrin, keratin K14

© 2018 Interdisciplinary Toxicology, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of AgNPs on the human reconstructed epidermis

Published Online: Oct 18, 2019

Page range: 289 - 293

Received: Nov 21, 2017

Accepted: Feb 23, 2018

DOI: https://doi.org/10.2478/intox-2018-0028

Keywordshuman reconstructed epidermis, AgNPs, IL-6, IL-8, loricrin, keratin K14

© 2018 Interdisciplinary Toxicology, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
human reconstructed epidermis, AgNPs, IL-6, IL-8, loricrin, keratin K14