<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Soybeans contain the isoflavone aglycone, an endocrine disrupter. To determine the effects of small amounts of isoflavones on developmental processes, we administered 6.25, 62.5, or 625 µg isoflavone per egg to early stage (stage 10) developing chick embryos via the yolk just beneath the embryo. Eggs were kept at 37±0.5 °C and >80% relative humidity, with one rotation per hour for 48 hrs. The embryos were observed under a stereomicroscope for morphological abnormalities and number of somites. Relative to control eggs, there were no significant differences in the average number of somites in eggs administered isoflavone aglycone. Isoflavone, however, had a dose associated effect on abnormal embryogenesis. Embryos treated with isoflavone aglycone showed developmental arrest not reaching somitegenesis, dysmorphology of the neural tube, and shortening of entire embryos.</p></abstract>

Soybeans contain the isoflavone aglycone, an endocrine disrupter. To determine the effects of small amounts of isoflavones on developmental processes, we administered 6.25, 62.5, or 625 µg isoflavone per egg to early stage (stage 10) developing chick embryos via the yolk just beneath the embryo. Eggs were kept at 37±0.5 °C and >80% relative humidity, with one rotation per hour for 48 hrs. The embryos were observed under a stereomicroscope for morphological abnormalities and number of somites. Relative to control eggs, there were no significant differences in the average number of somites in eggs administered isoflavone aglycone. Isoflavone, however, had a dose associated effect on abnormal embryogenesis. Embryos treated with isoflavone aglycone showed developmental arrest not reaching somitegenesis, dysmorphology of the neural tube, and shortening of entire embryos.

The developmental effects of isoflavone aglycone administration on early chick embryos

Interdisciplinary Toxicology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Soybeans contain the isoflavone aglycone, an endocrine disrupter. To determine the effects of small amounts of isoflavones on developmental processes, we...

The developmental effects of isoflavone aglycone administration on early chick embryos

Published Online: Oct 18, 2019

Page range: 236 - 239

Received: Jan 20, 2018

Accepted: Apr 17, 2018

DOI: https://doi.org/10.2478/intox-2018-0022

Keywords
isoflavone aglycone, estrogen receptor, chick embryo, somites, abnormality

© 2018 Interdisciplinary Toxicology, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The developmental effects of isoflavone aglycone administration on early chick embryos

Published Online: Oct 18, 2019

Page range: 236 - 239

Received: Jan 20, 2018

Accepted: Apr 17, 2018

DOI: https://doi.org/10.2478/intox-2018-0022

Keywordsisoflavone aglycone, estrogen receptor, chick embryo, somites, abnormality

© 2018 Interdisciplinary Toxicology, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
isoflavone aglycone, estrogen receptor, chick embryo, somites, abnormality