<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A straightforward LC-ESI-MS/MS method was developed and validated for the detection and quantitation of the herbicide glyphosate (GLY) and its metabolite aminomethyl phosphonic acid (AMPA) in honeybees. The method was validated, fulfilling the SANTE 11945/2015 guideline criteria, demonstrating acceptable mean recoveries at LOQ and 10×LOQ varying from 75-87% for both compounds. LOQ was determined at 0.2 and 0.5 μg/g <sub>bee body weight (bw)</sub> for GLY and AMPA respectively. Analysis of 14 honeybee samples displayed only one positive sample, containing GLY marginally above LOQ and traces of AMPA.</p></abstract>

A straightforward LC-ESI-MS/MS method was developed and validated for the detection and quantitation of the herbicide glyphosate (GLY) and its metabolite aminomethyl phosphonic acid (AMPA) in honeybees. The method was validated, fulfilling the SANTE 11945/2015 guideline criteria, demonstrating acceptable mean recoveries at LOQ and 10×LOQ varying from 75-87% for both compounds. LOQ was determined at 0.2 and 0.5 μg/g bee body weight (bw) for GLY and AMPA respectively. Analysis of 14 honeybee samples displayed only one positive sample, containing GLY marginally above LOQ and traces of AMPA.

Direct determination of glyphosate and aminomethyl phosphonic acid in honeybees

Benaki Phytopathological Institute, Department of Pesticides Control and Phytopharmacy

Hellenic Plant Protection Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Direct determination of glyphosate and aminomethyl phosphonic acid in honeybees"}

A straightforward LC-ESI-MS/MS method was developed and validated for the detection and quantitation of the herbicide glyphosate (GLY) and its metabolite...

Direct determination of glyphosate and aminomethyl phosphonic acid in honeybees

Published Online: Jan 24, 2018

Page range: 40 - 46

Received: Dec 22, 2017

Accepted: Jan 09, 2018

DOI: https://doi.org/10.2478/hppj-2018-0005

Keywords
AMPA, glyphosate, honeybees, LC-ESI-MS/MS, validation

© 2018 K.M. Kasiotis et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Direct determination of glyphosate and aminomethyl phosphonic acid in honeybees

Published Online: Jan 24, 2018

Page range: 40 - 46

Received: Dec 22, 2017

Accepted: Jan 09, 2018

DOI: https://doi.org/10.2478/hppj-2018-0005

KeywordsAMPA, glyphosate, honeybees, LC-ESI-MS/MS, validation

© 2018 K.M. Kasiotis et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
AMPA, glyphosate, honeybees, LC-ESI-MS/MS, validation