Open Access

Genetic variation of Taenia saginata cyst isolates from Iraq based on mitochondrial COX1 sequences

A. A. Mohammed
A. A. Mohammed
   | Dec 17, 2022
Helminthologia's Cover Image
About this article
Previous Article
Next Article
Cite
Article Category: Research Article
Published Online: Dec 17, 2022
Page range: 226 - 232
Received: Oct 29, 2021
Accepted: Aug 30, 2022
DOI: https://doi.org/10.2478/helm-2022-0030
Keywords
© 2022 A. A. Mohammed, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig.1

Phylogenetic relation of T. saginata from the present study and other taeniids. Phylogenetic tree was constructed using maximum likelihood method (Kimura’s 2-parameter model) based on partial COX1 sequences. Sequences reported in the present study are shown as IQTS-H1– IQTS-H5 with accession numbers (OK036447– OK036451), respectively.
Phylogenetic relation of T. saginata from the present study and other taeniids. Phylogenetic tree was constructed using maximum likelihood method (Kimura’s 2-parameter model) based on partial COX1 sequences. Sequences reported in the present study are shown as IQTS-H1– IQTS-H5 with accession numbers (OK036447– OK036451), respectively.

The five haplotypes of T. saginata COX1 sequences from Iraqi cattle were put to comparison with reference COX1 sequence from Korea (accession number AY684274) for the substitutions of the nucleotide and corresponding amino acid variations.

Haplotypes of the current study Position of nucleic acid substitution Amino acid substitution
IQTS-H1 –* –*
IQTS-H2 197 (A→G) Glu→Gly
332 (C→T) Ala→Val
IQTS-H3 70 (T→A) Leu→Met
356 (T→C) Ile→Thr
IQTS-H4 10 (G→C) Ala→Leu
371 (T→C) Leu→Ser
IQTS-H5 39 (C→T) Val→Val
146 (G→A) Arg→Lys
195 (G→C) Gln→His

T. saginata and other taeniids COX1 nucleotide sequences from GenBank were utilized for genetic diversity and phylogeny.

Taeniid parasites GenBank accession no. Origin Host Author (reference)
T. saginata AY684274 Korea Human Jeon et al., 2007
T. saginata AB533173 Thailand Human Okamoto, 2016 unpublished
T. saginata AB465242 Thailand Human Okamoto et al., 2010
T. saginata JQ756969–JQ756979 Iran Cattle Rostami et al., 2015
T. asiatica AB597287 Japan Human Yamasaki et al., 2021
T. solium AB066491 Ecuador Pig Nakao et al., 2002
T. multiceps JX535576 China Sheep Li et al., 2013
T. hydatigena MT784895 China Dog Ohiolei et al., 2021
Echinococcus granulosus MW214711 Iran Sheep Babaei et al., 2021

Pairwise evolutionary divergence in COX1 gene sequences between T. saginata and other taeniids.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
1 IQTS-H1:OK036447
2 IQTS-H2:OK036448 0.005
3 IQTS-H3:OK036449 0.005 0.011
4 IQTS-H4:OK036450 0.005 0.011 0.010
5 IQTS-H5:OK036451 0.008 0.013 0.013 0.013
6 T.saginata:JQ756969(Iran) 0.000 0.005 0.005 0.005 0.008
7 T.saginata:JQ756970(Iran) 0.003 0.008 0.003 0.008 0.011 0.003
8 T.saginata:JQ756971(Iran) 0.003 0.003 0.008 0.008 0.011 0.003 0.005
9 T.saginata:JQ756972(Iran) 0.003 0.008 0.008 0.003 0.010 0.003 0.005 0.005
10 T.saginata:JQ756973(Iran) 0.003 0.003 0.008 0.008 0.011 0.003 0.005 0.005 0.005
11 T.saginata:JQ756974(Iran) 0.003 0.008 0.008 0.003 0.011 0.003 0.005 0.005 0.005 0.005
12 T.saginata:JQ756975(Iran) 0.011 0.016 0.005 0.016 0.018 0.011 0.008 0.013 0.013 0.013 0.013
13 T.saginata:JQ756976(Iran) 0.005 0.011 0.011 0.011 0.013 0.005 0.008 0.008 0.008 0.008 0.008 0.016
14 T.saginata:JQ756977(Iran) 0.008 0.013 0.013 0.013 0.016 0.008 0.010 0.010 0.010 0.010 0.010 0.018 0.013
15 T.saginata:JQ756978(Iran) 0.013 0.018 0.018 0.018 0.010 0.013 0.016 0.016 0.016 0.016 0.016 0.024 0.018 0.018
16 T.saginata:JQ756979(Iran) 0.005 0.005 0.011 0.011 0.008 0.005 0.008 0.003 0.008 0.008 0.008 0.016 0.011 0.013 0.018
17 T.saginata:AB465242(Thailand) 0.003 0.008 0.008 0.008 0.011 0.003 0.005 0.005 0.005 0.005 0.005 0.013 0.008 0.010 0.016 0.008
18 T.saginata:AB533173(Thailand) 0.003 0.003 0.008 0.008 0.011 0.003 0.005 0.000 0.005 0.005 0.005 0.013 0.008 0.010 0.016 0.003 0.005
19 T.asiatica:AB597287(Japan) 0.027 0.027 0.032 0.032 0.035 0.027 0.029 0.024 0.029 0.029 0.029 0.038 0.032 0.035 0.038 0.027 0.029 0.024
20 T.saginata:AY684274(Korea) 0.000 0.005 0.005 0.005 0.008 0.000 0.003 0.003 0.003 0.003 0.003 0.011 0.005 0.008 0.013 0.005 0.003 0.003 0.027
21 T.solium:AB066491(Ecuador) 0.125 0.119 0.132 0.132 0.128 0.125 0.129 0.122 0.128 0.122 0.129 0.138 0.129 0.135 0.138 0.119 0.129 0.122 0.138 0.125
22 T.multiceps:JX535576(China) 0.058 0.058 0.063 0.063 0.066 0.058 0.061 0.055 0.060 0.061 0.061 0.069 0.063 0.066 0.072 0.058 0.061 0.055 0.075 0.058 0.107
23 T.hydatigena:MT784895(China) 0.124 0.127 0.131 0.131 0.134 0.124 0.127 0.127 0.127 0.124 0.127 0.137 0.124 0.134 0.137 0.131 0.127 0.127 0.143 0.124 0.146 0.134
24 E.granulosus:MW214711(Iran) 0.216 0.212 0.223 0.219 0.219 0.216 0.219 0.212 0.219 0.216 0.216 0.230 0.219 0.219 0.230 0.209 0.212 0.212 0.234 0.216 0.212 0.237 0.189

Depending on mitochondrial COX1 sequences and accession numbers, the distribution pattern related to T. saginata haplotypes in Iraqi cattle.

Taenia saginata haplotypes No. of samples (n) GenBank accession no.
IQTS-H1 17 OK036447
IQTS-H2 8 OK036448
IQTS-H3 6 OK036449
IQTS-H4 4 OK036450
IQTS-H5 2 OK036451
eISSN:
1336-9083
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Zoology, Ecology, other, Medicine, Clinical Medicine, Microbiology, Virology and Infection Epidemiology
Journal RSS Feed