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Ultrastructure and cytochemistry of the mature spermatozoon of Khawia armeniaca (Cholodkovsky, 1915) (Caryophyllidea: Lytocestidae), a parasite of Capoeta capoeta sevangi (De Filippi, 1865) (Teleostei, Cyprinidae)

M. Matoušková
M. Matoušková
, 
M. Bruňanská
M. Bruňanská
, 
J. Nebesářová
J. Nebesářová
 and 
L. G. Poddubnaya
L. G. Poddubnaya
   | Nov 19, 2020
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Article Category: Research Article
Published Online: Nov 19, 2020
Page range: 353 - 360
Received: Feb 05, 2020
Accepted: Jul 09, 2020
DOI: https://doi.org/10.2478/helm-2020-0040
Keywords
© 2020 M. Matoušková, M. Bruňanská, J. Nebesářová, L. G. Poddubnaya, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1

Cross sections of the mature spermatozoon of K. armeniaca: (a) vas deferens with five different regions of the spermatozoon (I-V); (b) region I presents one axoneme and six cortical microtubules; (c) region II shows cortical microtubules which are arranged in a semi-circle under the plasma membrane; (d-g) region III or nucleated region: small diameter of anterior part of the nucleus (d), which gradually increases (e), reaches a maximum in the middle part (f), and diminishes again more posteriorly (g); (h-j) region IV shows cortical microtubules which are arranged in two opposite rows (note one single microtubule in the middle between the two opposite rows) (h), rapidly reduced volume of cytoplasm and number of cortical microtubules (i, j); (k-l) region V with one axoneme which lost its central structure (k) and undergoes disorganization into doublets (l). Ax, axoneme; Arrowheads, attachment zones; BB, basal body of the cilium; CM, cortical microtubules; D, doublets; L, lamellae; N, nucleus. Scale bars: a = 1000 nm, b-h, l = 200 nm, i = 250 nm, j, k =125 nm.
Cross sections of the mature spermatozoon of K. armeniaca: (a) vas deferens with five different regions of the spermatozoon (I-V); (b) region I presents one axoneme and six cortical microtubules; (c) region II shows cortical microtubules which are arranged in a semi-circle under the plasma membrane; (d-g) region III or nucleated region: small diameter of anterior part of the nucleus (d), which gradually increases (e), reaches a maximum in the middle part (f), and diminishes again more posteriorly (g); (h-j) region IV shows cortical microtubules which are arranged in two opposite rows (note one single microtubule in the middle between the two opposite rows) (h), rapidly reduced volume of cytoplasm and number of cortical microtubules (i, j); (k-l) region V with one axoneme which lost its central structure (k) and undergoes disorganization into doublets (l). Ax, axoneme; Arrowheads, attachment zones; BB, basal body of the cilium; CM, cortical microtubules; D, doublets; L, lamellae; N, nucleus. Scale bars: a = 1000 nm, b-h, l = 200 nm, i = 250 nm, j, k =125 nm.

Fig. 2

Granules of glycogen in the cytoplasm of the mature spermatozoon of K. armeniaca after application of Thiéry method (1967): (a) cross section of region II; (b) cross section of region III; (c) cross section of region IV; (d) longitudinal section of region II. Ax, axoneme; CM, cortical microtubules; G, glycogen; N, nucleus. Scale bars: a-c = 200 nm, d = 250 nm.
Granules of glycogen in the cytoplasm of the mature spermatozoon of K. armeniaca after application of Thiéry method (1967): (a) cross section of region II; (b) cross section of region III; (c) cross section of region IV; (d) longitudinal section of region II. Ax, axoneme; CM, cortical microtubules; G, glycogen; N, nucleus. Scale bars: a-c = 200 nm, d = 250 nm.

Fig. 3

Schematic reconstruction of the mature spermatozoon of K. armeniaca. I–V, five different regions of the mature spermatozoon; ASE, anterior spermatozoon extremity; Ax, axoneme; AZ, attachment zones; CC, central core; CM, cortical microtubules; G, glycogen; N, nucleus; PM, plasma membrane; PSE, posterior spermatozoon extremity.
Schematic reconstruction of the mature spermatozoon of K. armeniaca. I–V, five different regions of the mature spermatozoon; ASE, anterior spermatozoon extremity; Ax, axoneme; AZ, attachment zones; CC, central core; CM, cortical microtubules; G, glycogen; N, nucleus; PM, plasma membrane; PSE, posterior spermatozoon extremity.

Variation of the maximum number of cortical microtubules (CM) in the spermatozoa of the Caryophyllidea.

Families Genus Species CM References
Capingentidae Breviscolex orientalis 15 Yoneva et al., 2011
Caryophyllaeidae Archigetes sieboldi 11 Bruňanská et al., 2019
Caryophyllaeus laticeps 15 Bruňanská & Kostič, 2012
Glaridacris catostomi Świderski & Mackiewicz, 2002
Hunturella nodulosa 10 Yoneva et al., 2012a
Wenyonia virilis 30 Gamil, 2008
Lytocestidae Atractolytocestus huronensis Bruňanská et al., 2011
Caryophyllaeides fennica 19 Matoušková et al., 2018
Khawia armeniaca 35 present study
Khawia rossittensis 22 Matoušková et al., 2019
Khawia sinensis 15 Bruňanská, 2009
Lytocestus indicus 25 Yoneva et al., 2012b
Monobothrioides chalmersius 40 Arafa & Hamada, 2004
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