<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Paragonimus proliferus</italic> (<italic>P. proliferus</italic>), one of 46 <italic>Paragonimus</italic> species registered in the National Center for Biotechnology Information database, may be much more widely distributed in Southeast Asia than previously thought, as its reported natural foci have increased in the past decades. However, very little is known about its molecular biology, especially at the transcriptome level. For the first time, the transcriptome of this species was sequenced and compared with four other common <italic>Paragonimus</italic> species, namely <italic>Paragonimus skrjabini</italic>, <italic>Paragonimus kellicotti</italic>, <italic>Paragonimus miyazakii</italic>, and <italic>Paragonimus westermani</italic>, to predict homologous genes and differentially expressed homologous genes to explore interspecies differences of <italic>Paragonimus proliferus</italic>.</p><p>A total of 7393 genes were found to be significantly differentially expressed. Of these, 49 were considered to be core genes because they were differentially expressed in all four comparison groups. Annotations revealed that these genes were related mainly to “duplication, transcription, or translation”, energy or nutrient metabolism, and parasitic growth, proliferation, motility, invasion, adaptation to the host, or virulence. Interestingly, a majority (5601/7393) of the identified genes, and in particular the core genes (48/49), were expressed at lower levels in <italic>P. proliferus</italic>. The identified genes may play essential roles in the biological differences between <italic>Paragonimus</italic> species. This work provides fundamental background information for further research into the molecular biology of <italic>P. proliferus</italic>.</p></abstract>

Paragonimus proliferus (P. proliferus), one of 46 Paragonimus species registered in the National Center for Biotechnology Information database, may be much more widely distributed in Southeast Asia than previously thought, as its reported natural foci have increased in the past decades. However, very little is known about its molecular biology, especially at the transcriptome level. For the first time, the transcriptome of this species was sequenced and compared with four other common Paragonimus species, namely Paragonimus skrjabini, Paragonimus kellicotti, Paragonimus miyazakii, and Paragonimus westermani, to predict homologous genes and differentially expressed homologous genes to explore interspecies differences of Paragonimus proliferus.A total of 7393 genes were found to be significantly differentially expressed. Of these, 49 were considered to be core genes because they were differentially expressed in all four comparison groups. Annotations revealed that these genes were related mainly to “duplication, transcription, or translation”, energy or nutrient metabolism, and parasitic growth, proliferation, motility, invasion, adaptation to the host, or virulence. Interestingly, a majority (5601/7393) of the identified genes, and in particular the core genes (48/49), were expressed at lower levels in P. proliferus. The identified genes may play essential roles in the biological differences between Paragonimus species. This work provides fundamental background information for further research into the molecular biology of P. proliferus.

Differentially expressed homologous genes reveal interspecies differences of <italic>Paragonimus proliferus</italic> based on transcriptome analysis

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Differentially expressed homologous genes reveal interspecies differences of  based on transcriptome analysis

Paragonimus proliferus (P. proliferus), one of 46 Paragonimus species registered in the National Center for Biotechnology Information database, may be much...

Differentially expressed homologous genes reveal interspecies differences of Paragonimus proliferus based on transcriptome analysis

Article Category: Research Article

Published Online: Aug 05, 2020

Page range: 196 - 210

Received: Nov 08, 2019

Accepted: Apr 01, 2020

DOI: https://doi.org/10.2478/helm-2020-0029

Keywords
transcriptome, homologous genes, interspecies differences

© 2020 S. H. Li, S. D. Li, H. J. Li, J. Y. Li, J. J. Xu, G. J. Chang, L. J. Yang, W. Q. Wang, Y. L. Zhang, Z. Q. Ma, S. M. He, W. L. Wang, H. L. Huang, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Differentially expressed homologous genes reveal interspecies differences of Paragonimus proliferus based on transcriptome analysis

Article Category: Research Article

Published Online: Aug 05, 2020

Page range: 196 - 210

Received: Nov 08, 2019

Accepted: Apr 01, 2020

DOI: https://doi.org/10.2478/helm-2020-0029

Keywordstranscriptome, homologous genes, interspecies differences

© 2020 S. H. Li, S. D. Li, H. J. Li, J. Y. Li, J. J. Xu, G. J. Chang, L. J. Yang, W. Q. Wang, Y. L. Zhang, Z. Q. Ma, S. M. He, W. L. Wang, H. L. Huang, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
transcriptome, homologous genes, interspecies differences