<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The aim of this study was to investigate whether Enzyme-Linked Immunosorbent Assays (ELISA) and Western Blotting (WB) methods could contribute to the assessment of clinical outcomes in genotype-defined cystic echinococcosis (CE) patients. Twenty-nine human isolates and blood samples have been taken from patients who underwent surgery or percutaneous aspiration (PAIR) for therapeutic purposes at Ege University and Manisa Celal Bayar University Hospitals. All sera of patients were screened for the presence of <italic>E. granulosus</italic> IgG antibodies using in-house approved ELISA and WB methods. According to the ELISA results, five patients had high, thirteen patients had medium and eight patients had low specific antibody level response which ranged 1/640 -1/5000. Despite confirmed WB positivity three patients were found to be negative by ELISA. Immunoblot analysis of EgAg showed many protein bands with size of 8, 12, 20, 22, 24, 36, 75 and 90 kDa. Among of them, 8 – 12 kDa bands (90 %), 20 – 22 kDa and 36 kDa bands presented strong reactivity against human serum specimens. No serum samples from healthy control reacted with EgAg. Phylogenetic analysis of resulting COX1 and NAD1sequences has revealed that all patients in our study were infected with the <italic>E. granulosus</italic> G1-G3 genotype. There was no consistent correlation between results of ELISA and WB, the number or size of cysts and genotype. Our study brings a unique contribution in terms of relationship between serological investigation, disease genotypes and clinical outcomes.</p></abstract>

The aim of this study was to investigate whether Enzyme-Linked Immunosorbent Assays (ELISA) and Western Blotting (WB) methods could contribute to the assessment of clinical outcomes in genotype-defined cystic echinococcosis (CE) patients. Twenty-nine human isolates and blood samples have been taken from patients who underwent surgery or percutaneous aspiration (PAIR) for therapeutic purposes at Ege University and Manisa Celal Bayar University Hospitals. All sera of patients were screened for the presence of E. granulosus IgG antibodies using in-house approved ELISA and WB methods. According to the ELISA results, five patients had high, thirteen patients had medium and eight patients had low specific antibody level response which ranged 1/640 -1/5000. Despite confirmed WB positivity three patients were found to be negative by ELISA. Immunoblot analysis of EgAg showed many protein bands with size of 8, 12, 20, 22, 24, 36, 75 and 90 kDa. Among of them, 8 – 12 kDa bands (90 %), 20 – 22 kDa and 36 kDa bands presented strong reactivity against human serum specimens. No serum samples from healthy control reacted with EgAg. Phylogenetic analysis of resulting COX1 and NAD1sequences has revealed that all patients in our study were infected with the E. granulosus G1-G3 genotype. There was no consistent correlation between results of ELISA and WB, the number or size of cysts and genotype. Our study brings a unique contribution in terms of relationship between serological investigation, disease genotypes and clinical outcomes.

A pilot comparative study between serological and genetic investigations in relationship to clinical outcomes on patients with cystic echinococcosis

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The aim of this study was to investigate whether Enzyme-Linked Immunosorbent Assays (ELISA) and Western Blotting (WB) methods could contribute to the...

A pilot comparative study between serological and genetic investigations in relationship to clinical outcomes on patients with cystic echinococcosis

Published Online: May 23, 2020

Page range: 91 - 99

Received: Apr 17, 2019

Accepted: Feb 11, 2020

DOI: https://doi.org/10.2478/helm-2020-0012

Keywords
Cystic echinococcosis, ELISA, western blotting, serology, sequence analysis

© 2020 Nu. Altintas, S. A. Karamil, O. Turkum, M. Akil, A. Sakarya, H. Bozkaya, J. Šoltys, Na. Altintas, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

A pilot comparative study between serological and genetic investigations in relationship to clinical outcomes on patients with cystic echinococcosis

Published Online: May 23, 2020

Page range: 91 - 99

Received: Apr 17, 2019

Accepted: Feb 11, 2020

DOI: https://doi.org/10.2478/helm-2020-0012

KeywordsCystic echinococcosis, ELISA, western blotting, serology, sequence analysis

© 2020 Nu. Altintas, S. A. Karamil, O. Turkum, M. Akil, A. Sakarya, H. Bozkaya, J. Šoltys, Na. Altintas, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Cystic echinococcosis, ELISA, western blotting, serology, sequence analysis