<abstract xmlns="http://www.w3.org/1999/xhtml"><p>This study conducted to determine relationship among and within Iranian <italic>Helichrysum</italic> species (Asteraceae). In this study based on ISSR markers, the highest percentage of ISSR loci polymorphism (54.7%) occurred in <italic>H</italic>. <italic>armenium</italic>. The highest gene diversity over loci (1.224), Shannon’s Information Index (0.224%) and Expected Heterozygosity (0.142%) occurred in <italic>H</italic>. <italic>armenium</italic> (0.18) and the lowest of these parameters (0%) were observed in <italic>H</italic>. <italic>araxinum</italic>, <italic>H</italic>. <italic>graveolens</italic>, <italic>H</italic>. <italic>persicum</italic> and <italic>H</italic>. <italic>psychrophilum</italic>. The highest genetic similarity occurred between <italic>H</italic>. <italic>armenium</italic> and <italic>H</italic>. <italic>rubicundum</italic> (0.989), while the lowest was between <italic>H</italic>. <italic>polyphyllum</italic> and <italic>H</italic>. <italic>graveolens (0.213)</italic>. The analysis of molecular variance (AMOVA), showed significant genetic variation among (24%) and within (76%) species. In morphological analysis traits such as indumentum, resting bud, achene length, achenial papillae, dimension of receptacle and form and apex of phyllaries were main diagnostic features. Results obtained from the morphological cluster were greatly consistent with the molecular data, to elucidating taxonomic relationships, as well as both attributed the higher diversity in <italic>H</italic>. <italic>armenium</italic> and <italic>H</italic>. <italic>rubicundum</italic> in comparison with other species and also indicated that <italic>H. persicum</italic> is a member of <italic>H</italic>. <italic>oocephalum</italic> species. Totally we confirmed the presence of 18 species in Iran.</p></abstract>

This study conducted to determine relationship among and within Iranian Helichrysum species (Asteraceae). In this study based on ISSR markers, the highest percentage of ISSR loci polymorphism (54.7%) occurred in H. armenium. The highest gene diversity over loci (1.224), Shannon’s Information Index (0.224%) and Expected Heterozygosity (0.142%) occurred in H. armenium (0.18) and the lowest of these parameters (0%) were observed in H. araxinum, H. graveolens, H. persicum and H. psychrophilum. The highest genetic similarity occurred between H. armenium and H. rubicundum (0.989), while the lowest was between H. polyphyllum and H. graveolens (0.213). The analysis of molecular variance (AMOVA), showed significant genetic variation among (24%) and within (76%) species. In morphological analysis traits such as indumentum, resting bud, achene length, achenial papillae, dimension of receptacle and form and apex of phyllaries were main diagnostic features. Results obtained from the morphological cluster were greatly consistent with the molecular data, to elucidating taxonomic relationships, as well as both attributed the higher diversity in H. armenium and H. rubicundum in comparison with other species and also indicated that H. persicum is a member of H. oocephalum species. Totally we confirmed the presence of 18 species in Iran.

Assessment of relationships among and within <italic>Helichrysum</italic> Mill. (<italic>Asteraceae</italic>) species by using ISSR markers and morphological traits

Forest and Rangeland Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center. AREEO

Research institute of Forests and Rangelands. AREEO

Department of Biology School of Science, Tehran, Science and Research Branch

Hacquetia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Assessment of relationships among and within  Mill. () species by using ISSR markers and morphological traits

This study conducted to determine relationship among and within Iranian Helichrysum species (Asteraceae). In this study based on ISSR markers, the highest...

Assessment of relationships among and within Helichrysum Mill. (Asteraceae) species by using ISSR markers and morphological traits

Published Online: Feb 14, 2019

Page range: 105 - 118

Received: Apr 18, 2018

Accepted: Dec 19, 2018

DOI: https://doi.org/10.2478/hacq-2018-0014

Keywords
ISSR Marker, Morphology, Population, Iran

© 2019 Narjes Azizi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Assessment of relationships among and within Helichrysum Mill. (Asteraceae) species by using ISSR markers and morphological traits

Published Online: Feb 14, 2019

Page range: 105 - 118

Received: Apr 18, 2018

Accepted: Dec 19, 2018

DOI: https://doi.org/10.2478/hacq-2018-0014

KeywordsISSR Marker, Morphology, Population, Iran

© 2019 Narjes Azizi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
ISSR Marker, Morphology, Population, Iran