1. bookVolume 48 (2021): Issue 1 (May 2021)
Journal Details
License
Format
Journal
eISSN
1338-7014
First Published
16 Apr 2017
Publication timeframe
2 times per year
Languages
English
access type Open Access

Drought tolerance of three ethnomedicinal shrubs evaluated based on their seed germination rates at different drought levels induced by using polyethylene glycol (PEG6000)

Published Online: 01 Mar 2021
Volume & Issue: Volume 48 (2021) - Issue 1 (May 2021)
Page range: 49 - 54
Received: 14 Apr 2020
Accepted: 23 Aug 2020
Journal Details
License
Format
Journal
eISSN
1338-7014
First Published
16 Apr 2017
Publication timeframe
2 times per year
Languages
English
Abstract

Sideritis incana, Stachys ocymastrum, and Thymus fontanesii are medicinal plants belonging to the Lamiaceae family and occurring in semi-arid lands in northern Algeria and in many other countries along the Mediterranean coastline. Despite the ecological and economic interests and also the questionable future these species may meet in their natural habitats, various aspects of their seed biology have not been recognised to this date. This study was intended for in situ conservation of these plants. The problem investigated was the germination response of seeds to different water potential levels attained with using different amounts of polyethylene glycol (PEG6000) (0, –0.03, –0.07, –0.2, –0.5, –1 and –1.6 MPa). In this way, the appropriate conditions and the threshold tolerance of seed germination against water stress were assessed. Seeds of the three species lacked primary/innate dormancy and they germinated abundant and fast in distilled water (S. incana – 65%; S. ocymastrum – 60% and T. fontanseii – 90%). Small seeds of T. fontanesii tolerated more water stress and germinated under up to –1 MPa (–10 bars). Large seeds of S. incana and S. ocymastrum, however, were more sensitive to the drought stress and germinated only under –0.5 MPa (–5 bars). Moreover, more studied parameters were found developing negative reponse under rising drought stress, such as postponed triggering of seed germination, decreased germination velocity and prolonged germination duration, as well as the average time of germination. Therefore, these three plants appear to postpone their establishment until arrival of conditions promising for germination, including sufficient rainfall.

Keywords

Abbad, A., Belaqziz, R., Bekkouche, K., Markou, M., 2011. Influence of temperature and water potential on laboratory germination of two Moroccan endemic thymes: Thymus maroccanus Ball. and Thymus broussonetii Boiss. African Journal of Agricultural Research, 6: 4740–4745. doi: 10.5897/AJAR10.574 Search in Google Scholar

Al-Taisan, W.A., Al-Qarawi, A.A., Alsubiee, M.S., 2010. Effect of water stress by Polyethylene Glycol 8000 and Sodium Chloride on germination of Ephedra alata Decne seeds. Saudi Journal of Biological Sciences, 17: 253–257. https://doi.org/10.1016/j.sjbs.2010.04.01110.1016/j.sjbs.2010.04.011373072823961087 Search in Google Scholar

Blum, A., 2015. Stress, strain, signalling, and adaptation – not just a matter of definition. Journal of Experimental Botany, 67: 562–565. https://doi.org/10.1093/jxb/erv49710.1093/jxb/erv49726585225 Search in Google Scholar

Calonje, M., Kay, J., Griffith, M.P., 2011. Propagation of cycad collections from seed. Applied reproductive biology for conservation. Sibbaldia, 9: 77–96. https://doi.org/10.23823/Sibbaldia/2011.123 Search in Google Scholar

Cheurfa, M., Allem, R., Sebaihia, M., Belhireche, S., 2013. Effet de l’huile essentielle de Thymus vulgaris sur les bactéries pathogènes responsables de gastroentérites (Effect of essential oil of Thymus vulgaris on bacterial pathogens responsible for gastroenteritis). Phytothérapie, 11: 154. https://doi.org/10.1007/s10298-013-0777-710.1007/s10298-013-0777-7 Search in Google Scholar

Cochrane, J.A., Hoyl, G.L., Yates, C.J., Wood, J., Nicotra, A.B., 2015. Climate warming delays and decreases seedling emergence in a Mediterranean ecosystem. Oikos, 124: 150–160. https://doi.org/10.1111/oik.0135910.1111/oik.01359 Search in Google Scholar

Dadach, M., Mehdadi, Z., 2018a. How short-term storage affects seed germination feature of three mediterranean medicinal plants. Journal of Biology and Nature, 9 (4): 127–135. Search in Google Scholar

Dadach, M., Mehdadi, Z., 2018b. Germination responses of Ballota hirsuta seeds under conditions of temperature, salinity and water stress. Hellenic Plant Protection Journal, 11: 34–39. https://doi.org/10.2478/hppj-2018-000410.2478/hppj-2018-0004 Search in Google Scholar

Dadach, M., Mehdadi, Z., Adjouj, A., 2018. Evaluation of the tolerance to temperature and salinity on seed germination of three Lamiacea from Algerian Scrubland. Advanced Research in Life Sciences, 2 (1): 11–15. https://doi.org/10.1515/arls-2018-002110.1515/arls-2018-0021 Search in Google Scholar

Dadach, M., Mehdadi, Z., Latreche, A., 2015. Effect of water stress on seed germination of Thymus serpyllum L. from Tessala mount. Journal of Plant Sciences, 10 (4): 151–158. doi: 10.3923/jps.2015.151.15810.3923/jps.2015.151.158 Search in Google Scholar

Donohue, K., Rubio de Casas, R., Burghardt, L., Kovach, K., Willis, C.G., 2010. Germination, postgermination adaptation, and species ecological ranges. Annual Review of Ecology, Evolution, and Systematics, 41: 293–319. https://doi.org/10.1146/annurev-ecolsys-102209-14471510.1146/annurev-ecolsys-102209-144715 Search in Google Scholar

Faisal, S., Mujtaba, S.M., Mahboob, A.W., 2019. Polyethylene Glycol mediated osmotic stress impacts on growth and biochemical aspects of wheat (Triticum aestivum L.). Journal of Crop Science and Biotechnology, 22 (3): 213–223. https://doi.org/10.1007/s12892-018-0166-010.1007/s12892-018-0166-0 Search in Google Scholar

Fernandez-Calzado, M.R., Mesa, M.J., Merzouki, A., Porcel, C.M., 2012. Vascular plant diversity and climate change in the upper zone of Sierra Nevada, Spain. Plant Biosystems, 146: 1044–1053. https://doi.org/10.1080/11263504.2012.71027310.1080/11263504.2012.710273 Search in Google Scholar

Galmés, J., Medrano, H., Flexas, J., 2006. Germination capacity and temperature dependence in Mediterranean species of the Balearic Islands. Investigación Agraria, Sistemas y Recursos Forestales, 15: 88–95. doi: 10.5424/srf/2006151-0095510.5424/srf/2006151-00955 Search in Google Scholar

Gill, P.K., Sharma, A.D., Singh, P., Bhullar, S.S., 2003. Changes in germination, growth and soluble sugar contents of Sorghum bicolor (L.) Moench seeds under various abiotic stresses. Plant Growth Regulation, 40: 157–162. https://doi.org/10.1023/A:102425222237610.1023/A:1024252222376 Search in Google Scholar

Gorai, M., El Aloui, W., Yang, X., Neffati, M., 2014. Toward understanding the ecological role of mucilage in seed germination of a desert shrub Henophyton deserti: interactive effects of temperature, salinity and osmotic stress. Plant and Soil, 374: 727–738. https://doi.org/10.1007/s11104-013-1920-910.1007/s11104-013-1920-9 Search in Google Scholar

Khan, M.A., Ungar, I.A., 1984. The effect of salinity and temperature on the germination of polymorphic seeds and growth of Atriplex triangularis Willd. American Journal of Botany, 71 (4): 481–489. doi: 10.2307/244332310.1002/j.1537-2197.1984.tb12533.x Search in Google Scholar

Krichen, K., Ben Mariem, H., Chaieb, M., 2014. Ecophysiological requirements on seed germination of a Mediterranean perennial grass (Stipa tenacissima L.) under controlled temperatures and water stress. South African Journal of Botany, 94: 210–217. https://doi.org/10.1016/j.sajb.2014.07.00810.1016/j.sajb.2014.07.008 Search in Google Scholar

Lakhal, H., Boudiar, T., Kabouche, A.S., Laggoune, A., Kabouche, Z., Topcu, G., 2011. Antioxidant activity and flavonoids of Stachys ocymastrum. Chemistry of Natural Compounds, 46: 964965. https://doi.org/10.1007/s10600-011-9797-410.1007/s10600-011-9797-4 Search in Google Scholar

Medail, F., Quezel, P., 1997. Hot-spots analysis for conservation of plant biodiversity in the Mediterranean basin. Annals of the Missouri Botanical Garden, 84: 112–127.10.2307/2399957 Search in Google Scholar

Pavia, I., Rocha, L., Moutinho-Pereira, J., Lima-Brito, J., Carlos Correia, C., 2019. Screening for drought resistance during germination of modern and old Iberian wheat cultivars. Acta Botanica Croatica, 78 (2): 169–174. doi: 10.2478/botcro-2019-001210.2478/botcro-2019-0012 Search in Google Scholar

Ranal, M.A., Santana, D.G., 2006. How and why to measure the germination process. Revista Brasileira de Botânica, 29: 1–11. http://dx.doi.org/10.1590/S0100-8404200600010000210.1590/S0100-84042006000100002 Search in Google Scholar

Rey, A., Pegoraro, E., Oyonarte, C., Were, A., Escribano, P., Raimundo, J., 2011. Impact of land degradation on soil respiration in a steppe (Stipa tenacissima L.) semi-arid ecosystem in the SE of Spain. Soil Biology and Biochemistry, 43: 393–403. https://doi.org/10.1016/j.soilbio.2010.11.00710.1016/j.soilbio.2010.11.007 Search in Google Scholar

Shepherd, K.A., Macfarlane, T.D., Colmer, T.D., 2005. Morphology, anatomy and histochemistry of Salicornioideae (Chenopodiaceae) fruits and seeds. Annals of Botany, 95: 917–933. https://doi.org/10.1093/aob/mci10110.1093/aob/mci101424674815760916 Search in Google Scholar

Stromberg, J.C., Boudell, J.A., 2013. Floods, drought, and seed mass of riparian plant species. Journal of Arid Environments, 97: 99–107. https://doi.org/10.1016/j.jaridenv.2013.05.01210.1016/j.jaridenv.2013.05.012 Search in Google Scholar

Underwood, E.C., Viers, J.H., Klausmeyer, K.R., Cox, R.L. Shaw, M.R., 2009. Threats and biodiversity in the Mediterranean Biome. Diversity and Distributions, 15 (2): 188–197. https://doi.org/10.1111/j.1472-4642.2008.00518.x10.1111/j.1472-4642.2008.00518.x Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo