<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The goal of this research was to evaluate the effect of light, temperature, sucrose and PEG on the growth of <italic>Fragaria vesca in vitro</italic> and the expression of regulatory <italic>Myb10, WD40</italic> and enzyme-coding genes <italic>CHI, CHS, DFR, EGL, F3H</italic> and <italic>UFGT</italic>, which are essential for anthocyanin biosynthesis. We observed plants’ response to osmotic stress, the decrease in growth and microshoot weight. A change in the expression of the investigated genes was evident under the suboptimal concentration of sucrose. The addition of PEG to the medium caused a decrease in microshoot weight and gene expression. Blue + red lights of the LED lighting system significantly affected microshoot growth <italic>in vitro</italic>. Red and blue + red + UV lights slightly reduced microshoot weight and caused a reddish colour of petioles, which indicate increased anthocyanin synthesis. Moreover, most of the studied genes’ expression tended to increase when shoots were exposed to blue, blue + red and blue + red + UV lights. A temperature of 15°C (vs 22°C) significantly reduced the mean fresh weight of microshoots while increasing <italic>CHI</italic> and <italic>CHS</italic> gene expression and decreasing <italic>WD40</italic> gene expression. Exposure to a higher temperature (30°C) induced the vitrification of microshoots, although the fresh weight did not differ from that of the control. Gene expression also depended on the duration of exposure. In the case of <italic>CHS</italic>, gene expression remained the same or increased after exposure for 1 week and then decreased after exposure for 4 weeks.</p>
</abstract>

The goal of this research was to evaluate the effect of light, temperature, sucrose and PEG on the growth of Fragaria vesca in vitro and the expression of regulatory Myb10, WD40 and enzyme-coding genes CHI, CHS, DFR, EGL, F3H and UFGT, which are essential for anthocyanin biosynthesis. We observed plants’ response to osmotic stress, the decrease in growth and microshoot weight. A change in the expression of the investigated genes was evident under the suboptimal concentration of sucrose. The addition of PEG to the medium caused a decrease in microshoot weight and gene expression. Blue + red lights of the LED lighting system significantly affected microshoot growth in vitro. Red and blue + red + UV lights slightly reduced microshoot weight and caused a reddish colour of petioles, which indicate increased anthocyanin synthesis. Moreover, most of the studied genes’ expression tended to increase when shoots were exposed to blue, blue + red and blue + red + UV lights. A temperature of 15°C (vs 22°C) significantly reduced the mean fresh weight of microshoots while increasing CHI and CHS gene expression and decreasing WD40 gene expression. Exposure to a higher temperature (30°C) induced the vitrification of microshoots, although the fresh weight did not differ from that of the control. Gene expression also depended on the duration of exposure. In the case of CHS, gene expression remained the same or increased after exposure for 1 week and then decreased after exposure for 4 weeks.

The effect of osmotic stress, lighting spectrum and temperature on growth and gene expression related to anthocyanin biosynthetic pathway in wild strawberry (<italic>Fragaria vesca</italic> L.) <italic>in vitro</italic>

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The effect of osmotic stress, lighting spectrum and temperature on growth and gene expression related to anthocyanin biosynthetic pathway in wild strawberry ( L.) 

The goal of this research was to evaluate the effect of light, temperature, sucrose and PEG on the growth of Fragaria vesca in vitro and the expression of...

The effect of osmotic stress, lighting spectrum and temperature on growth and gene expression related to anthocyanin biosynthetic pathway in wild strawberry (Fragaria vesca L.) in vitro

Article Category: ORIGINAL ARTICLE

Published Online: Dec 31, 2023

Page range: 419 - 431

Received: Oct 26, 2022

Accepted: Oct 24, 2023

DOI: https://doi.org/10.2478/fhort-2023-0030

Keywords
controlled conditions, flavonoids, growth, metabolism, pigmentation, stress

© 2023 Jurgita Vinskienė et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The effect of osmotic stress, lighting spectrum and temperature on growth and gene expression related to anthocyanin biosynthetic pathway in wild strawberry (Fragaria vesca L.) in vitro

Article Category: ORIGINAL ARTICLE

Published Online: Dec 31, 2023

Page range: 419 - 431

Received: Oct 26, 2022

Accepted: Oct 24, 2023

DOI: https://doi.org/10.2478/fhort-2023-0030

Keywordscontrolled conditions, flavonoids, growth, metabolism, pigmentation, stress

© 2023 Jurgita Vinskienė et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
controlled conditions, flavonoids, growth, metabolism, pigmentation, stress