<abstract xmlns="http://www.w3.org/1999/xhtml">

<italic>Murrayae exotica</italic> is a traditional Chinese medicine widely grown in southeast China. A Box–Behnken design of response surface methodology was employed to further optimise ultrasonic-assisted extraction conditions for <italic>M. exotica</italic> leaves total flavonoids (MELTF). The results showed that the optimised extraction conditions were an ultrasonic power of 240 W, an ultrasonic temperature of 60 °C, a solvent concentration of 76%, an ultrasonic time of 55 min and a liquid–solid ratio of 22 mL · g−1. Under these conditions, 8.59 ± 0.34 mg · g−1 was achieved as the mean experimental value of extraction yield, which amounts to 2.56 times that of ethanol leaching extraction. As compared to MELTF, MELTF after purification (MELPTF) had a higher purity of 9.96%, which was nearly nine times higher than that of MELTF (1.26%). As compared to MELTF, MELPTF had higher α-glucosidase and α-amylase inhibitory activities as well as DPPH· and ABTS·+ scavenging activities with IC50 values of 0.021, 0.094, 0.245 and 0.113 mg · mL−1, which are 1.33, 2.12, 3.17 and 1.78 times higher than those of MELTF (0.028, 0.199, 0.777 and 0.201 mg · mL−1). The study thus demonstrates the eligibility of MELPTF to be considered as a multifunctional bioactive ingredient having potential applications in anti-hyperglycaemic pharmaceutical formulation and as an antioxidant in functional foods.
</abstract>

Murrayae exotica is a traditional Chinese medicine widely grown in southeast China. A Box–Behnken design of response surface methodology was employed to further optimise ultrasonic-assisted extraction conditions for M. exotica leaves total flavonoids (MELTF). The results showed that the optimised extraction conditions were an ultrasonic power of 240 W, an ultrasonic temperature of 60 °C, a solvent concentration of 76%, an ultrasonic time of 55 min and a liquid–solid ratio of 22 mL · g−1. Under these conditions, 8.59 ± 0.34 mg · g−1 was achieved as the mean experimental value of extraction yield, which amounts to 2.56 times that of ethanol leaching extraction. As compared to MELTF, MELTF after purification (MELPTF) had a higher purity of 9.96%, which was nearly nine times higher than that of MELTF (1.26%). As compared to MELTF, MELPTF had higher α-glucosidase and α-amylase inhibitory activities as well as DPPH· and ABTS·+ scavenging activities with IC50 values of 0.021, 0.094, 0.245 and 0.113 mg · mL−1, which are 1.33, 2.12, 3.17 and 1.78 times higher than those of MELTF (0.028, 0.199, 0.777 and 0.201 mg · mL−1). The study thus demonstrates the eligibility of MELPTF to be considered as a multifunctional bioactive ingredient having potential applications in anti-hyperglycaemic pharmaceutical formulation and as an antioxidant in functional foods.

Optimisation of ultrasonic-assisted extraction and biological activity of total flavonoids from leaves of <italic>Murrayae exotica</italic> using response surface methodology

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Optimisation of ultrasonic-assisted extraction and biological activity of total flavonoids from leaves of  using response surface methodology

Murrayae exotica is a traditional Chinese medicine widely grown in southeast China. A Box–Behnken design of response surface methodology was employed to...

Optimisation of ultrasonic-assisted extraction and biological activity of total flavonoids from leaves of Murrayae exotica using response surface methodology

Article Category: ORIGINAL ARTICLE

Published Online: Jun 26, 2023

Page range: 135 - 148

Received: Dec 27, 2022

Accepted: Feb 14, 2023

DOI: https://doi.org/10.2478/fhort-2023-0010

Keywords
anti-hyperglycaemic activity, leaves, response surface optimisation, total flavonoids, ultrasonic-assisted extraction

© 2023 Yao Wen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Optimisation of ultrasonic-assisted extraction and biological activity of total flavonoids from leaves of Murrayae exotica using response surface methodology

Article Category: ORIGINAL ARTICLE

Published Online: Jun 26, 2023

Page range: 135 - 148

Received: Dec 27, 2022

Accepted: Feb 14, 2023

DOI: https://doi.org/10.2478/fhort-2023-0010

Keywordsanti-hyperglycaemic activity, leaves, response surface optimisation, total flavonoids, ultrasonic-assisted extraction

© 2023 Yao Wen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
anti-hyperglycaemic activity, leaves, response surface optimisation, total flavonoids, ultrasonic-assisted extraction