<abstract xmlns="http://www.w3.org/1999/xhtml">

In recent years, there has been increasing interest in floral volatiles due to their important function in reproduction, self-protection and so on. Although some progress was made on deciphering emissions of floral volatiles and on related mechanisms under a variety of environmental factors, the effects of Zn2+ concentrations on mitochondrial function and floral volatile emissions are yet to be revealed. <italic>Dendrobium huoshanense</italic> petals were used as materials and were treated with a 0–8 mM ZnSO4 solution. Intracellular Zn2+ concentrations were evaluated by the fluorescent dye method and atomic absorption method. Mitochondrial respiratory chain complex activities and the precursor and ATP contents were determined by the biochemical method. Floral volatile components were analyzed by the gas chromatograph-mass spectrometer (GC-MS). The results indicated that ZnSO4 application significantly increased intracellular Zn2+ concentrations. Elevated intracellular Zn2+ concentrations differently affected mitochondrial respiratory chain complex activities, precursor and adenosine triphosphate (ATP) productions and floral volatile emissions. Moreover, positive correlations exist among the activities of mitochondrial respiratory chain complexes, productions of precursors and ATP and emissions of floral volatiles. It is concluded that Zn2+ concentrations induce the activity changes of mitochondrial respiratory chain complexes, especially complex II and V, which promote or inhibit the emissions of floral volatiles by affecting the precursor and ATP levels that are closely related to the production of terpenoids, benzoids and fatty acid derivatives. The research will contribute to understanding the relationship between Zn2+ concentrations and floral volatile emissions from the perspective of mitochondrial function.
</abstract>

In recent years, there has been increasing interest in floral volatiles due to their important function in reproduction, self-protection and so on. Although some progress was made on deciphering emissions of floral volatiles and on related mechanisms under a variety of environmental factors, the effects of Zn2+ concentrations on mitochondrial function and floral volatile emissions are yet to be revealed. Dendrobium huoshanense petals were used as materials and were treated with a 0–8 mM ZnSO4 solution. Intracellular Zn2+ concentrations were evaluated by the fluorescent dye method and atomic absorption method. Mitochondrial respiratory chain complex activities and the precursor and ATP contents were determined by the biochemical method. Floral volatile components were analyzed by the gas chromatograph-mass spectrometer (GC-MS). The results indicated that ZnSO4 application significantly increased intracellular Zn2+ concentrations. Elevated intracellular Zn2+ concentrations differently affected mitochondrial respiratory chain complex activities, precursor and adenosine triphosphate (ATP) productions and floral volatile emissions. Moreover, positive correlations exist among the activities of mitochondrial respiratory chain complexes, productions of precursors and ATP and emissions of floral volatiles. It is concluded that Zn2+ concentrations induce the activity changes of mitochondrial respiratory chain complexes, especially complex II and V, which promote or inhibit the emissions of floral volatiles by affecting the precursor and ATP levels that are closely related to the production of terpenoids, benzoids and fatty acid derivatives. The research will contribute to understanding the relationship between Zn2+ concentrations and floral volatile emissions from the perspective of mitochondrial function.

Zn2+ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in <italic>Dendrobium huoshanense</italic>

Engineering Technology Research Center for Plant Cell of Anhui Province

Department of Food Science and Technology, College of Light Industry and Food Engineering

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Zn induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in 

In recent years, there has been increasing interest in floral volatiles due to their important function in reproduction, self-protection and so on. Although...

Zn²⁺ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in Dendrobium huoshanense

Article Category: Original Article

Published Online: Jun 02, 2022

Page range: 105 - 124

Received: Aug 12, 2021

Accepted: Apr 25, 2022

DOI: https://doi.org/10.2478/fhort-2022-0009

Keywords
ATP, floral volatiles, mitochondrial respiratory chain complexes, precursors, Zn

© 2022 Wangsheng Zhu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Zn2+ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in Dendrobium huoshanense

Article Category: Original Article

Published Online: Jun 02, 2022

Page range: 105 - 124

Received: Aug 12, 2021

Accepted: Apr 25, 2022

DOI: https://doi.org/10.2478/fhort-2022-0009

KeywordsATP, floral volatiles, mitochondrial respiratory chain complexes, precursors, Zn

© 2022 Wangsheng Zhu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Zn²⁺ induces changes in activities of mitochondrial respiratory chain complexes and emissions of floral volatiles in Dendrobium huoshanense

Keywords
ATP, floral volatiles, mitochondrial respiratory chain complexes, precursors, Zn