<abstract xmlns="http://www.w3.org/1999/xhtml">

Pollen characteristics are very important for <italic>Iris</italic> interspecific hybridisation. In this study, the pollen viability and male meiosis were studied in yellow-flowered <italic>Iris dichotoma</italic> (Y2), <italic>I. domestica</italic> (S3) and their hybrids F1, F2 and BC1 (BC1-Y and BC1-S). The BC1-Y hybrids showed higher pollen viability than that of F1, F2 and BC1-S hybrids, which were between <italic>I. dichotoma</italic> (26.1%) and <italic>I. domestica</italic> (35.1%). Two sterile hybrids, F2-1 and BC1-S-1, exhibited more meiotic abnormalities (57.3% and 58.7%) than other individuals. During the first meiotic division, a diffuse diplotene stage was observed for the first time in the genus <italic>Iris</italic>. The meiotic abnormalities included non-congressed chromosomes, chromosome bridges, lagging chromosomes, unequal division, abnormally oriented spindle fibres, nonsynchronous division and polyad, and resulted in reduced pollen fertility. The relatively high frequency of 2n pollen grains was found in hybrids of BC1-Y-2, BC1-Y-1, BC1-S-2, BC1-S-3 and BC1-S-4. Our research provides a new resource for meiotic behaviour and pollen fertility of the genus <italic>Iris</italic>.
</abstract>

Pollen characteristics are very important for Iris interspecific hybridisation. In this study, the pollen viability and male meiosis were studied in yellow-flowered Iris dichotoma (Y2), I. domestica (S3) and their hybrids F1, F2 and BC1 (BC1-Y and BC1-S). The BC1-Y hybrids showed higher pollen viability than that of F1, F2 and BC1-S hybrids, which were between I. dichotoma (26.1%) and I. domestica (35.1%). Two sterile hybrids, F2-1 and BC1-S-1, exhibited more meiotic abnormalities (57.3% and 58.7%) than other individuals. During the first meiotic division, a diffuse diplotene stage was observed for the first time in the genus Iris. The meiotic abnormalities included non-congressed chromosomes, chromosome bridges, lagging chromosomes, unequal division, abnormally oriented spindle fibres, nonsynchronous division and polyad, and resulted in reduced pollen fertility. The relatively high frequency of 2n pollen grains was found in hybrids of BC1-Y-2, BC1-Y-1, BC1-S-2, BC1-S-3 and BC1-S-4. Our research provides a new resource for meiotic behaviour and pollen fertility of the genus Iris.

Meiotic behaviour and pollen fertility of F1, F2 and BC1 progenies of <italic>Iris dichotoma</italic> and <italic>I. domestica</italic>

School of Advanced Agriculture and Bioengineering

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Meiotic behaviour and pollen fertility of F, F and BC progenies of  and 

Pollen characteristics are very important for Iris interspecific hybridisation. In this study, the pollen viability and male meiosis were studied in...

Meiotic behaviour and pollen fertility of F₁, F₂ and BC₁ progenies of Iris dichotoma and I. domestica

Article Category: Original Article

Published Online: May 15, 2021

Page range: 173 - 183

Received: Feb 07, 2021

Accepted: Apr 09, 2021

DOI: https://doi.org/10.2478/fhort-2021-0013

Keywords
2n gametes, abnormal meiosis, interspecific hybrid, male

© 2021 Wenji Xu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Meiotic behaviour and pollen fertility of F1, F2 and BC1 progenies of Iris dichotoma and I. domestica

Article Category: Original Article

Published Online: May 15, 2021

Page range: 173 - 183

Received: Feb 07, 2021

Accepted: Apr 09, 2021

DOI: https://doi.org/10.2478/fhort-2021-0013

Keywords2n gametes, abnormal meiosis, interspecific hybrid, male

© 2021 Wenji Xu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Meiotic behaviour and pollen fertility of F₁, F₂ and BC₁ progenies of Iris dichotoma and I. domestica

Keywords
2n gametes, abnormal meiosis, interspecific hybrid, male