<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In this study, the full cDNA sequences of HcADH2 and HcADH3 were cloned from <italic>Hedychium coronarium</italic>. The amino acid sequences encoded by them contained three most conserved motifs of short-chain alcohol dehydrogenase (ADH), namely NAD<sup>+</sup> binding domain, TGxxx[AG]xG and active site YxxxK. The highest similarity between two genes and ADH from other plants was 70%. Phylogenetic analysis showed that they belonged to a member of the short-chain dehydrogenases/reductases 110C subfamily, but they were distinctly clustered in different clades. Real-time polymerase chain reaction analyses showed that <italic>HcADH2</italic> was specifically expressed in bract, and it was expressed higher in no-scented <italic>Hedychium forrestii</italic> than other <italic>Hedychium</italic> species, but was undetectable in <italic>Hedychium coccineum</italic>. <italic>HcADH3</italic> was expressed higher in the lateral petal of the flower than in other vegetative organs, and it was expressed the most in <italic>H. coronarium</italic> that is the most scented among <italic>Hedychium</italic> species, and its expression levels peaked at the half opening stage. <italic>HcADH2</italic> and <italic>HcADH3</italic> had almost no significant expression in leaves, but <italic>HcADH2</italic> was expressed in response to external stimuli. The mechanical injury and methyl jasmonate (MeJA) treatment could induce expression of <italic>HcADH2</italic> in leaves, whereas <italic>HcADH3</italic> could have an induced expression only by MeJA. The recombinant HcADH3 protein, but not HcADH2, expressed in <italic>Escherichia coli</italic>-catalysed conversion of geraniol into citral. It was speculated that HcADH3 had an induced expression in vegetative organ of <italic>H. coronarium</italic> and took part in monoterpenoid biosynthesis in <italic>H. coronarium</italic> flowers, but the role of HcADH2 is relevant only for defensive reactions.</p></abstract>

In this study, the full cDNA sequences of HcADH2 and HcADH3 were cloned from Hedychium coronarium. The amino acid sequences encoded by them contained three most conserved motifs of short-chain alcohol dehydrogenase (ADH), namely NAD+ binding domain, TGxxx[AG]xG and active site YxxxK. The highest similarity between two genes and ADH from other plants was 70%. Phylogenetic analysis showed that they belonged to a member of the short-chain dehydrogenases/reductases 110C subfamily, but they were distinctly clustered in different clades. Real-time polymerase chain reaction analyses showed that HcADH2 was specifically expressed in bract, and it was expressed higher in no-scented Hedychium forrestii than other Hedychium species, but was undetectable in Hedychium coccineum. HcADH3 was expressed higher in the lateral petal of the flower than in other vegetative organs, and it was expressed the most in H. coronarium that is the most scented among Hedychium species, and its expression levels peaked at the half opening stage. HcADH2 and HcADH3 had almost no significant expression in leaves, but HcADH2 was expressed in response to external stimuli. The mechanical injury and methyl jasmonate (MeJA) treatment could induce expression of HcADH2 in leaves, whereas HcADH3 could have an induced expression only by MeJA. The recombinant HcADH3 protein, but not HcADH2, expressed in Escherichia coli-catalysed conversion of geraniol into citral. It was speculated that HcADH3 had an induced expression in vegetative organ of H. coronarium and took part in monoterpenoid biosynthesis in H. coronarium flowers, but the role of HcADH2 is relevant only for defensive reactions.

Differential expression and functional analysis of two short-chain alcohol dehydrogenases/reductases in <italic>Hedychium coronarium</italic>

Department of Landscape Architecture, College of Life Science

The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture

Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Differential expression and functional analysis of two short-chain alcohol dehydrogenases/reductases in 

In this study, the full cDNA sequences of HcADH2 and HcADH3 were cloned from Hedychium coronarium. The amino acid sequences encoded by them contained three...

Differential expression and functional analysis of two short-chain alcohol dehydrogenases/reductases in Hedychium coronarium

Article Category: Research Article

Published Online: May 18, 2020

Page range: 97 - 114

Received: Jan 30, 2020

Accepted: Apr 14, 2020

DOI: https://doi.org/10.2478/fhort-2020-0010

Keywords
expression analysis, function, short-chain alcohol dehydrogenase/reductase

© 2020 Hua Chen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Differential expression and functional analysis of two short-chain alcohol dehydrogenases/reductases in Hedychium coronarium

Article Category: Research Article

Published Online: May 18, 2020

Page range: 97 - 114

Received: Jan 30, 2020

Accepted: Apr 14, 2020

DOI: https://doi.org/10.2478/fhort-2020-0010

Keywordsexpression analysis, function, short-chain alcohol dehydrogenase/reductase

© 2020 Hua Chen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
expression analysis, function, short-chain alcohol dehydrogenase/reductase