<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The current study investigated the effects of gamma radiation on the death rate, morphological traits and meiotic abnormalities in ground-grown chrysanthemum ‘Donglinruixue’, and inter-simple sequence repeat (ISSR) markers were used to identify the DNA polymorphism among mutants. The results showed that the death rate significantly increased with increase in radiation dose. Semi-lethal (LD50) dose was approximately 35 Gy. Compared with unirradiated control, plant growth was significantly inhibited. After irradiation, a series of morphological variations and cytological aberrations occurred in radiated plants. The peak in variation frequency appeared at 35 Gy. In total, ISSR analysis produced 72 scorable bands, of which 64 (88.89%) were polymorphic. The current study demonstrated that gamma irradiation generates a sufficient number of induced mutations and that ISSR analysis offered a useful molecular marker analysis for the identification of mutants.</p></abstract>

The current study investigated the effects of gamma radiation on the death rate, morphological traits and meiotic abnormalities in ground-grown chrysanthemum ‘Donglinruixue’, and inter-simple sequence repeat (ISSR) markers were used to identify the DNA polymorphism among mutants. The results showed that the death rate significantly increased with increase in radiation dose. Semi-lethal (LD50) dose was approximately 35 Gy. Compared with unirradiated control, plant growth was significantly inhibited. After irradiation, a series of morphological variations and cytological aberrations occurred in radiated plants. The peak in variation frequency appeared at 35 Gy. In total, ISSR analysis produced 72 scorable bands, of which 64 (88.89%) were polymorphic. The current study demonstrated that gamma irradiation generates a sufficient number of induced mutations and that ISSR analysis offered a useful molecular marker analysis for the identification of mutants.

Morphological, cytological and molecular variations induced by gamma rays in <italic>Chrysanthemum morifolium</italic> ‘Donglinruixue’

Folia Horticulturae

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Morphological, cytological and molecular variations induced by gamma rays in  ‘Donglinruixue’

The current study investigated the effects of gamma radiation on the death rate, morphological traits and meiotic abnormalities in ground-grown chrysanthemum...

Morphological, cytological and molecular variations induced by gamma rays in Chrysanthemum morifolium ‘Donglinruixue’

Article Category: Research Article

Published Online: May 18, 2020

Page range: 87 - 96

Received: Dec 13, 2019

Accepted: Mar 31, 2020

DOI: https://doi.org/10.2478/fhort-2020-0009

Keywords
death rate, DNA, gamma irradiation, ground-grown chrysanthemum, ISSR, meiosis

© 2020 Ling Wang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Morphological, cytological and molecular variations induced by gamma rays in Chrysanthemum morifolium ‘Donglinruixue’

Article Category: Research Article

Published Online: May 18, 2020

Page range: 87 - 96

Received: Dec 13, 2019

Accepted: Mar 31, 2020

DOI: https://doi.org/10.2478/fhort-2020-0009

Keywordsdeath rate, DNA, gamma irradiation, ground-grown chrysanthemum, ISSR, meiosis

© 2020 Ling Wang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
death rate, DNA, gamma irradiation, ground-grown chrysanthemum, ISSR, meiosis