<abstract xmlns="http://www.w3.org/1999/xhtml">In this study, the influence of gibberellic acid (GA3) on strawberry in vitro shoot culture growth and development was investigated. ‘Senga Sengana’ and ‘Elsanta’ clones were grown on the medium recommended by Boxus (1999), supplemented with BA (0.5 mg dm-3), IBA (0.1 mg dm-3), glucose (40.0 g dm-3) and GA3 (0.1 - control, 1.0, 2.0, 5.0, 10.0 mg dm-3). In general, gibberellic acid improved axillary shoot elongation and reduced the growth of callus as well as the formation of roots and the development of adventitious shoots. GA3 applied at a concentration of 1.0-2.0 mg dm-3 significantly increased the number of axillary shoots (mainly crown shoots), whereas under higher (5.0-10.0 mg dm-3) doses it stimulated the development of runners. It seems that a new method of strawberry micropropagation based on the multiplication of axillary crowns and runners, which could also reduce the risk of domination of in vitro cultures with adventitious shoots, might be elaborated</abstract>

In this study, the influence of gibberellic acid (GA3) on strawberry in vitro shoot culture growth and development was investigated. ‘Senga Sengana’ and ‘Elsanta’ clones were grown on the medium recommended by Boxus (1999), supplemented with BA (0.5 mg dm-3), IBA (0.1 mg dm-3), glucose (40.0 g dm-3) and GA3 (0.1 - control, 1.0, 2.0, 5.0, 10.0 mg dm-3). In general, gibberellic acid improved axillary shoot elongation and reduced the growth of callus as well as the formation of roots and the development of adventitious shoots. GA3 applied at a concentration of 1.0-2.0 mg dm-3 significantly increased the number of axillary shoots (mainly crown shoots), whereas under higher (5.0-10.0 mg dm-3) doses it stimulated the development of runners. It seems that a new method of strawberry micropropagation based on the multiplication of axillary crowns and runners, which could also reduce the risk of domination of in vitro cultures with adventitious shoots, might be elaborated

Development of <italic>in vitro</italic> shoot cultures of strawberry (<italic>Fragaria</italic> × <italic>ananassa</italic> Duch.) ‘Senga Sengana’ and ‘Elsanta’ under the influence of high doses of gibberellic acid

Folia Horticulturae

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Development of  shoot cultures of strawberry ( ×  Duch.) ‘Senga Sengana’ and ‘Elsanta’ under the influence of high doses of gibberellic acid

<trans-abstract xml:lang="pl" xmlns="http://www.w3.org/1999/xhtml"> W doświadczeniu badano wpływ kwasu giberelowego (GA3) na wzrost i rozwój pędowych kultur in vitro truskawki. Klony ‘Senga Sengana’ i ‘Elsanta’ były prowadzone na pożywce Boxusa (1999) uzupełnionej o BA (0,5 mg dm-3), IBA (0,1 mg dm-3), glukozę (40,0 g dm-3) i GA3 (0,1 - kontrola: 1,0; 2,0; 5,0; 10,0 mg dm-3). Kwas giberelowy pobudzał elongację pędów kątowych i osłabiał wzrost kalusa oraz tworzenie korzeni i pędów przybyszowych. Użyty w stężeniu 1,0-2,0 mg dm-3 zwiększał proliferację rozet kątowych, zaś w dawkach wyższych (5,0-10,0 mg dm-3) stymulował rozwój rozłogów in vitro. Umożliwia to opracowanie nowej metody mikrorozmnażania truskawki za pomocą rozet i rozłogów kątowych, która jednocześnie zmniejszy ryzyko opanowywania kultur przez pędy pochodzenia przybyszowego</trans-abstract>

W doświadczeniu badano wpływ kwasu giberelowego (GA3) na wzrost i rozwój pędowych kultur in vitro truskawki. Klony ‘Senga Sengana’ i ‘Elsanta’ były prowadzone na pożywce Boxusa (1999) uzupełnionej o BA (0,5 mg dm-3), IBA (0,1 mg dm-3), glukozę (40,0 g dm-3) i GA3 (0,1 - kontrola: 1,0; 2,0; 5,0; 10,0 mg dm-3). Kwas giberelowy pobudzał elongację pędów kątowych i osłabiał wzrost kalusa oraz tworzenie korzeni i pędów przybyszowych. Użyty w stężeniu 1,0-2,0 mg dm-3 zwiększał proliferację rozet kątowych, zaś w dawkach wyższych (5,0-10,0 mg dm-3) stymulował rozwój rozłogów in vitro. Umożliwia to opracowanie nowej metody mikrorozmnażania truskawki za pomocą rozet i rozłogów kątowych, która jednocześnie zmniejszy ryzyko opanowywania kultur przez pędy pochodzenia przybyszowego

In this study, the influence of gibberellic acid (GA3) on strawberry in vitro shoot culture growth and development was investigated. ‘Senga Sengana’ and...

Development of in vitro shoot cultures of strawberry (Fragaria × ananassa Duch.) ‘Senga Sengana’ and ‘Elsanta’ under the influence of high doses of gibberellic acid

Published Online: Aug 01, 2013

Page range: 43 - 52

DOI: https://doi.org/10.2478/fhort-2013-0137

Keywords
adventitious shoots, axillary shoots, propagation in vitro, runners

© 2009 Wojciech Litwińczuk et al., published by Versita

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Development of in vitro shoot cultures of strawberry (Fragaria × ananassa Duch.) ‘Senga Sengana’ and ‘Elsanta’ under the influence of high doses of gibberellic acid

Published Online: Aug 01, 2013

Page range: 43 - 52

DOI: https://doi.org/10.2478/fhort-2013-0137

Keywordsadventitious shoots, axillary shoots, propagation in vitro, runners

© 2009 Wojciech Litwińczuk et al., published by Versita

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
adventitious shoots, axillary shoots, propagation in vitro, runners