1. bookVolume 52 (2018): Issue 4 (October 2018)
Journal Details
First Published
30 Mar 2016
Publication timeframe
4 times per year
access type Open Access

Association between adiponectin rs17300539 and rs266729 gene polymorphisms with serum adiponectin level in an Iranian diabetic/pre-diabetic population

Published Online: 22 Nov 2018
Volume & Issue: Volume 52 (2018) - Issue 4 (October 2018)
Page range: 176 - 184
Journal Details
First Published
30 Mar 2016
Publication timeframe
4 times per year

Objective. Adiponectin is an adipokine that is mostly secreted from adipose tissues and has a significant role in the improvement of insulin resistant and type 2 diabetes mellitus (T2DM). This study is conducted to examine the association of rs17300539 and rs266729 with T2DM and serum adiponectin level in Iranian population. Method. A case-control study was conducted on 80 individuals with fasting plasma glucose (FPG) >100 (mg/dl) as diabetic-pre-diabetic group, and 80 individuals with fasting plasma glucose 70-100 (mg/dl) as control group. DNA extraction was done on samples and genotyping method was performed by PCR-RFLP. Result. The frequency of GA genotype in rs17300539 (diabetic/pre-diabetic 35.5%, control 11.3%, (OR [95%CI]=4.18[1.8-9.6]; p=0.001) and allele A (diabetic/pre-diabetic 31%, control 9%, (OR [95% CI]=4.67[2-10.7]) was significantly more in diabetic/pre-diabetic group compared to control group. The difference in the genotype frequency for rs266729 in diabetic group compared to that in control was not significant. The levels of adiponectin in diabetic cases had no difference compared to the control group in both polymorphisms. The rs266729 was not associated with any metabolic parameter except waist circumference (p=0.03), however, rs17300539 shows association only with fasting plasma glucose, triglyceride, and total cholesterol (p=0.007, 0.039, 0.0032, respectively). Conclusion. Our findings showed that there is an association between rs17300539 with the increase of T2DM but rs266729 showed no association with the risk of T2DM. Allele A of rs17300539 increased the risk of diabetes. There is no association between adiponectin level and both polymorphisms.


Abdalla MMI, Soon SC. Salivary adiponectin concentration in healthy adult males in relation to anthropometric measures and fat distribution. Endocr Regul 51, 185-192, 2017.2923219110.1515/enr-2017-001929232191Search in Google Scholar

Alberti KG, Genuth S, Bennett P, Buse J, Defronzo R, Hahn R, Kitzmiller J, Knowler WC, Lebovitz H, Lernmark A, Nathan D, Palmer J, Rizza R, Saudek C, Shaw J, Steffes M, Stern M, Tuomilehto J, Zimmet P; Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. Follow-up report on the diagnosis of diabetes mellitus. Diabetes Care 26, 3160-3167, 2003.10.2337/diacare.26.11.316014578255Open DOISearch in Google Scholar

Aleidi S, Issa A, Bustanji H, Khalil M, Bustanji Y. Adiponectin serum levels correlate with insulin resistance in type 2 diabetic patients. Saudi Pharm J 23, 250-256, 2014.10.1016/j.jsps.2014.11.011447581326106273Search in Google Scholar

Al-Azzam SI, Khabour OF, Alzoubi KH, Ghanma MW, Alhasan AY. The role of TNF-α G-308A promoter polymorphism in glycemic control in Type 2 diabetes patients. J Endocrinol Invest 37, 113-118, 2014.10.1007/s40618-013-0002-424497209Search in Google Scholar

An SS, Hanley AJ, Ziegler JT, Brown WM, Haffner SM, Norris JM, Rotter JI, Guo X, Chen YD, Wagenknecht LE, Langefeld CD, Bowden DW, Palmer ND. Association between ADIPOQ SNPs with plasma adiponectin and glucose homeostasis and adiposity phenotypes in the IRAS Family Study. Mol Genet Metab 107, 721-728, 2012.10.1016/j.ymgme.2012.10.003350419523102667Search in Google Scholar

Bouzid T, Hamel FG, Lim JY. Role of adipokines in controlling insulin signaling pathways in type-2 diabetes and obesity. Current and future perspectives. Int J Diabetes Res 5, 75-85, 2016.Search in Google Scholar

Cefalu WT. Insulin resistance: cellular and clinical concepts. Exp Biol Med (Maywood) 226, 13-26, 2001.10.1177/15353702012260010311368233Search in Google Scholar

Chiodini BD, Specchia C, Gori F, Barlera S, D’Orazio A, Pietri S, Crociati L, Nicolucci A, Franciosi M, Signorini S, Brambilla P, Grazia Franzosi M; GISSI Prevenzione Investigators; SiBioC-GISSI Prevenzione Group. Adiponectin gene polymorphisms and their effect on the risk of myocardial infarction and type 2 diabetes: an association study in an Italian population. Ther Adv Cardiovasc Dis 4, 223-230, 2010.10.1177/175394471037148320576642Open DOISearch in Google Scholar

Cho J, Kim J, Shin J, Shin J, Yoon K. β‐cell mass in people with type 2 diabetes. J Diabetes Investig 2, 6-17, 2011.10.1111/j.2040-1124.2010.00072.x400801024843456Search in Google Scholar

Chu H, Wang M, Zhong D, Shi D, Ma L, Tong N, Zhang Z. AdipoQ polymorphisms are associated with type 2 diabetes mellitus: a meta-analysis study. Diabetes Metab Res Rev 29, 532-545, 2013.2365333510.1002/dmrr.242423653335Search in Google Scholar

Davis SK, Gebreab SY, Xu R, Riestra P, Khan RJ, Sumner AE, Hickson D, Bidulescu A. Association of adiponectin with type 2 diabetes and hypertension in African American men and women: the Jackson Heart Study. BMC Cardiovasc Disord 15, 13, 2015.10.1186/s12872-015-0005-5435499925885320Search in Google Scholar

Eissa A. Investigate the relation between Adiponectin gene variants and cardiovascular comorbidities and diabetes. Int J Health Sci (Qassim) 10, 183-189, 2016.Search in Google Scholar

Erfanian S, Moradzadeh M, Solhjoo K, Jahromi AS. Data describing the association between rs266729 polymorphism in adiponectin promoter gene and Type 2 Diabetes Mellitus. Data Brief 9, 1138-1140, 2016.10.1016/j.dib.2016.11.040513347027942572Search in Google Scholar

Eshraghi P, Hedayati M, Daneshpour M, Mirmiran, P, Azizi F. Association of body mass index and Trp64Arg polymorphism of the beta3-adrenoreceptor gene and leptin level in Tehran Lipid and Glucose Study. Br J Biomed Sci 64, 117-120, 2007.10.1080/09674845.2007.1173276917910280Open DOISearch in Google Scholar

Flegal KM, Kit BK, Graubard BI. Body mass index categories in observational studies of weight and risk of death. Am J Epidemiol 180, 288-296, 2014.10.1093/aje/kwu111473288024893710Search in Google Scholar

Gong M, Long J, Liu Q, Deng HC. Association of the ADIPOQ rs17360539 and rs266729 polymorphisms with Type 2 diabetes: A meta-analysis. Mol Cell Endocrinol 325, 78-83, 2010.10.1016/j.mce.2010.05.00720580771Search in Google Scholar

Han LY, Wu QH, Jiao ML, Hao YH, Liang LB, Gao LJ, Legge DG, Quan H, Zhao MM, Ning N, Kang Z, Sun H. Associations between single nucleotide polymorphisms (+45T>G, +276G>T, -11377C>G, -11391G>A) of adiponectin gene and type 2 diabetes mellitus: a systematic review and meta-analysis. Diabetologia 54, 2303-2314, 2011.10.1007/s00125-011-2202-921638131Search in Google Scholar

Hasani-Ranjbar S, Amoli M M, Tabatabaei-Malazy O, Rumi Y, Tavakkoly-Bazzaz J, Samimi H, Abbasifarid E. Effect of adiponectin gene polymorphisms on waist circumference in patients with diabetes. J of Diabetes and Metab Dis, 11, 14, 2012.Search in Google Scholar

Henneman P, Aulchenko YS, Frants RR, Zorkoltseva IV, Zillikens MC, Frolich M, Oostra BA, van Dijk KW, van Duijn CM. Genetic architecture of plasma adiponectin overlaps with the genetics of metabolic syndromerelated traits. Diabetes Care 33, 908-913, 2010.10.2337/dc09-1385284505020067957Open DOISearch in Google Scholar

Hsiao TJ, Lin E. A validation study of adiponectin rs266729 gene variant with Type 2 diabetes, obesity, and metabolic phenotypes in a Taiwanese population. Biochem Genet 54, 830-841, 2016.10.1007/s10528-016-9760-y27388775Search in Google Scholar

Ismail JM, Hussain MK, Mohammad HJ. Association of adiponectin gene polymorphism rs266729 with adiponectin levels in type 2 diabetic patients with and without CVD. In AL-NAJAF governorate, IRAQ. Int. J. of Adv. Res 4, 1089-1096, 2016.10.21474/IJAR01/211Search in Google Scholar

Kaftan AN, Hussain MK. Association of adiponectin gene polymorphism rs266729 with type two diabetes mellitus in Iraqi population. A pilot study. Gene 570, 95-99, 2015.10.1016/j.gene.2015.06.00426051417Search in Google Scholar

Khan M. M, Sonkar GK, Alam R, Singh S, Mehrotra S, Sonkar SK. Association of ADIPOQ gene variant rs266729 with circulatory adiponectin levels in patients with type 2 diabetes in North Indian population: A casecontrol study. Biomed Pharmacol J 10, 2017.10.13005/bpj/1123Search in Google Scholar

Laumen H, Saningong AD, Heid IM, Hess J, Herder C, Claussnitzer M, Baumert J, Lamina C, Rathmann W, Sedlmeier EM, Klopp N, Thorand B, Wichmann HE, Illig T, Hauner H. Functional characterization of promoter variants of the adiponectin gene complemented by epidemiological data. Diabetes 58, 984-991, 2009.10.2337/db07-164619074982266157719074982Open DOISearch in Google Scholar

Li YY, Yang ZJ, Zhou CW, Wang XM, Qian Y, Xu J, Wang B, Wu J. Adiponectin-11377CG gene polymorphism and type 2 diabetes mellitus in the Chinese population: A meta-analysis of 6425 subjects. PLoS ONE 8, e61153, 2013.10.1371/journal.pone.0061153362196823585875Search in Google Scholar

Lo JC, Ljubicic S, Leibiger B, Kern M, Leibiger IB, Moede T, Kelly ME, Chatterjee Bhowmick D, Murano I, Cohen P, Banks AS, Khandekar MJ, Dietrich A, Flier JS, Cinti S, Bluher M, Danial NN, Berggren PO, Spiegelman BM. Adipsin is an adipokine that improves β cell function in diabetes. Cell 158, 41-53, 2014.10.1016/j.cell.2014.06.005412819724995977Search in Google Scholar

Masson S, Gori F, Latini R, Milani V, Flyvbjerg A, Frystyk J, Crociati L, Pietri S, Vago T, Barlera S, Maggioni AP, Tognoni G, Tavazzi L, Omland T, Franzosi MG; GISSI-HF Investigators. Adiponectin in chronic heart failure: influence of diabetes and genetic variants. Eur J Clin Invest 41, 1330-1338, 2011.2162377810.1111/j.1365-2362.2011.02548.x21623778Search in Google Scholar

Mente A, Meyre D, Lanktree MB, Heydarpour M, Davis AD, Miller R, Gerstein H, Hegele RA, Yusuf S, Anand SS; SHARE Investigators; SHARE-AP Investigators. Causal relationship between adiponectin and metabolic traits: a Mendelian randomization study in a multiethnic population. PLoS One 8, e66808, 2013.10.1371/journal.pone.0066808369127723826141Search in Google Scholar

Menzaghi C, Salvemini L, Paroni G, De Bonis C, Mangiacotti D, Fini G, Doria A, Di Paola R, Trischitta V. Circulating HMW adiponectin isoform is heritable and shares a common genetic background with insulin resistance in non diabetic White Caucasians from Italy: evidence from a family-based study. J Intern Med 267, 287-294, 2010.10.1111/j.1365-2796.2009.02141.x283322819761474Search in Google Scholar

Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16, 1215, 1988.10.1093/nar/16.3.12153347653344216Search in Google Scholar

Morandi A, Maffeis C, Lobbens S, Bouatia-Naji N, Heude B, Pinelli L, Meyre D, Froguel P. Early detrimental metabolic outcomes of rs17300539-A allele of ADIPOQ gene despite higher adiponectinemia. Obesity (Silver Spring) 18, 1469-1473, 2010.10.1038/oby.2009.40319893502Search in Google Scholar

Ogedengbe S, Ezeani IU, Aihanuwa E. Comparison of clinical and biochemical variables in type 2 diabetes mellitus patients and their first-degree relatives with metabolic syndrome in Benin City, Nigeria: A cross sectional case controlled study. Endocr Regul 50, 32-40, 2016.2756063510.1515/enr-2016-000727560635Search in Google Scholar

Olckers A, Towers GW, van der Merwe A, Schwarz PE, Rheeder P, Schutte AE. Protective effect against type 2 diabetes mellitus identified within the ACDC gene in a black South African diabetic cohort. Metabolism 56, 587-592, 2007.10.1016/j.metabol.2006.10.00417445531Open DOISearch in Google Scholar

Ong KL, Li M, Tso AW, Xu A, Cherny SS, Sham PC, Tse HF, Lam TH, Cheung BM, Lam KS; Investigators of the Hong Kong Cardiovascular Risk Factor Prevalence Study. Association of genetic variants in the adiponectin gene with adiponectin level and hypertension in Hong Kong Chinese. Eur J Endocrinol 163, 251-257, 2010.10.1530/EJE-10-0251Search in Google Scholar

Prakash J, Mittal B, Awasthi S, Srivastava N. Association of adiponectin gene polymorphism with adiponectin levels and risk for insulin resistance syndrome. Int J Prev Med 6, 31, 2015.10.4103/2008-7802.154773Search in Google Scholar

Ramya K, Ayyappa KA, Ghosh S, Mohan V, Radha V. Genetic association of ADIPOQ gene variants with type 2 diabetes, obesity and serum adiponectin levels in south Indian population. Gene 532, 253-262, 2013.10.1016/j.gene.2013.09.012Search in Google Scholar

Reaven GM. Banting lecture 1988. Role of insulin resistance in human disease. Diabetes 37, 1595-1607, 1988.10.2337/diabetes.37.12.1595Search in Google Scholar

Renju VC, Santha K, Sethupathy S, Koshy M, Marichamy G, Sri Kumaran N. Serum adiponectin and fasting insulin levels in patients with type 2 diabetics. J Pharm Sci Res 4, 1844-1847, 2012.Search in Google Scholar

Sheng T, Yang K. Adiponectin and its association with insulin resistance and type 2 diabetes. J Genet Genomics 35, 321-326, 2008.10.1016/S1673-8527(08)60047-818571119Open DOISearch in Google Scholar

Siitonen N, Pulkkinen L, Lindstrom J, Kolehmainen M, Eriksson JG, Venojarvi M, Ilanne-Parikka P, Keinanen- Kiukaanniemi S, Tuomilehto J, Uusitupa M. Association of ADIPOQ gene variants with body weight, type 2 diabetes and serum adiponectin concentrations: the Finnish Diabetes Prevention Study. BMC Med Genet 12, 5, 2011.2121960210.1186/1471-2350-12-5303265521219602Search in Google Scholar

Tohidi M, Ghasemi A, Hadaegh F, Derakhshan A, Chary A, Azizi F. Age- and sex-specific reference values for fasting serum insulin levels and insulin resistance/sensitivity indices in healthy Iranian adults: Tehran Lipid and Glucose Study. Clin Biochem 47, 432-438, 2014.10.1016/j.clinbiochem.2014.02.00724530467Open DOISearch in Google Scholar

Ukkola O, Santaniemi M, Rankinen T, Leon AS, Skinner JS, Wilmore JH, Rao DC, Bergman R, Kesaniemi YA, Bouchard C. Adiponectin polymorphisms, adiposity and insulin metabolism: HERITAGE family study and Oulu diabetic study. Ann Med 37, 141-150, 2005.10.1080/0785389051000724116028335Search in Google Scholar

Vasseur F, Helbecque N, Dina C, Lobbens S, Delannoy V, Gaget S, Boutin P, Vaxillaire M, Lepretre F, Dupont S, Hara K, Clement K, Bihain B, Kadowaki T, Froguel P. Single-nucleotide polymorphism haplotypes in the both proximal promoter and exon 3 of the APM1 gene modulate adipocyte-secreted adiponectin hormone levels and contribute to the genetic risk for type 2 diabetes in French Caucasians. Hum Mol Genet 11, 2607-2614, 2002.10.1093/hmg/11.21.260712354786Open DOISearch in Google Scholar

Vollenweider P, Menard B, Nicod P. Insulin resistance, defective insulin receptor substrate 2-associated phosphatidylinositol- 3’ kinase activation, and impaired atypical protein kinase C (zeta/lambda) activation in myotubes from obese patients with impaired glucose tolerance. Diabetes 51, 1052-1059, 2002.10.2337/diabetes.51.4.105211916925Search in Google Scholar

Xu X, Wen J, Lu Y, Ji H, Su Y, Zhuang J, Su Y, Liu B, Li H, Xu Y. Impact of age on plasma vaspin concentration in a group of normal Chinese people. J Endocrinol Invest, 40(2):143-151, 2017.10.1007/s40618-016-0533-6526946927604490Open DOISearch in Google Scholar

Yamamoto S, Matsushita Y, Nakagawa T, Hayashi T, Noda M, Mizoue T. Circulating adiponectin levels and risk of type 2 diabetes in the Japanese. Nutr Diabetes 4, e130, 2014.10.1038/nutd.2014.27415117525133442Open DOISearch in Google Scholar

Yu JG, Javorschi S, Hevener AL, Kruszynska YT, Norman RA, Sinha M, Olefsky JM. The effect of thiazolidinediones on plasma adiponectin levels in normal, obese, and type 2 diabetic subjects. Diabetes 51, 2968-2974, 2002.10.2337/diabetes.51.10.296812351435Search in Google Scholar

Zadjali F, Al-Yahyaee S, Hassan MO, Albarwani S, Bayoumi RA. Association of adiponectin promoter variants with traits and clusters of metabolic syndrome in Arabs: family-based study. Gene 527, 663-669, 2013.10.1016/j.gene.2013.06.05723845780Search in Google Scholar

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