1. bookVolume 52 (2018): Issue 3 (July 2018)
Journal Details
License
Format
Journal
eISSN
1336-0329
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
access type Open Access

Salivary cortisol and nitrite concentrations in school teachers: A longitudinal pilot study

Published Online: 09 Aug 2018
Volume & Issue: Volume 52 (2018) - Issue 3 (July 2018)
Page range: 128 - 133
Journal Details
License
Format
Journal
eISSN
1336-0329
First Published
30 Mar 2016
Publication timeframe
4 times per year
Languages
English
Abstract

Objective. Occupational/mental stressors, which may be evaluated with measurements of salivary cortisol concentrations, affect the endothelial function and has implications on cardiovascular health. Nitric oxide (NO) is known to have an important role in cardiac function and may also be assessed in the saliva, but its participation in physiological responses to hypothalamic-pituitary-adrenal (HPA) axis stimulation is still not completely understood. The aim of the present study was to test the hypothesis that salivary NO (as nitrite) and cortisol concentrations in school teachers vary throughout the academic year.

Methods. Saliva samples were collected from 13 teachers distributed across five secondary schools. The samples were collected at 6:30 a.m., 11:30 a.m., and 5:30 p.m. in the months of March, July, and December. Salivary cortisol levels were measured by enzyme immunoassay (EIA), and salivary NO concentration was determined by the quantification of nitrite. The area under the curve in relation to ground (AUC) was calculated to assess the cortisol and nitrite concentrations throughout the day.

Results. No significant changes were observed in cortisol or nitrite concentrations across the three periods of the school year, while cortisol and nitrite levels showed a low positive and significant correlation (r=0.3455, p=0.0336).

Conclusion. The results suggest that changes in salivary cortisol levels are accompanied by changes in salivary nitrite levels. This is the first time that such association has been demonstrated. These results encourage further studies aimed to confirm the importance of salivary NO measurement in relation to occupational stress and cardiovascular health.

Keywords

Aardal E, Holm A. Cortisol in saliva - reference ranges in relation to cortisol in serum. Eur J Clin Chem Clin Biochem 33, 927–932, 1995.884542410.1515/cclm.1995.33.12.9278845424Search in Google Scholar

Agai-Demjaha T, Bislimovska JK, Mijakoski D. Level of work-related stress among teachers in elementary schools. Open Access Maced J Med Sci 15, 484–488, 2015.10.3889/oamjms.2015.076487784427275275Search in Google Scholar

Al-Ayadhi LY. Neurohormonal changes in medical students during academic stress. Ann Saudi Med 25, 36–40, 2005.10.5144/0256-4947.2005.36615056315822492Search in Google Scholar

Areias MEQ, Guimaraes LAM. Genero e estresse em trabalhadores de uma universidade publica do Estado de Sao Paulo. Psicologia em Estudo 9, 255–262, 2004.10.1590/S1413-73722004000200011Search in Google Scholar

Bangasser DA, Valentino RJ. Sex differences in molecular and cellular substrates of stress. Cell Mol Neurobiol 32,709–723, 2012.10.1007/s10571-012-9824-4337892022488525Open DOISearch in Google Scholar

Chou PH, Lin WH, Hung CA, Chang CC, Li WR, Lan TH, Huang MW. Perceived occupational stress is associated with decreased cortical activity of the prefrontal cortex: A multichannel near-infrared spectroscopy study. Sci Rep 6, 39089–39093, 2016.10.1038/srep39089515361827958364Open DOISearch in Google Scholar

Donald JA, Forgan LG, Cameron MS. The evolution of nitric oxide signalling in vertebrate blood vessels. J Comp Physiol B 185, 153–171, 2015.10.1007/s00360-014-0877-125502832Search in Google Scholar

Duckles SP, Miller VM. Hormonal modulation of endothelial NO production. Pflugers Arch 459, 841–851, 2010.10.1007/s00424-010-0797-1286557320213497Search in Google Scholar

Esch T, Stefano GB, Fricchione GL, Benson H. Stress-related diseases – a potential role for nitric oxide. Med Sci Monit 8, RA103–118, 2002.Search in Google Scholar

Farah C, Michel LYM, Balligand JL. Nitric oxide signalling in cardiovascular health and disease. Nat Rev Cardiol 15, 292–316, 2018.10.1038/nrcardio.2017.22429388567Open DOISearch in Google Scholar

Fekedulegn DB, Andrew ME, Burchfiel CM, Violanti JM, Hartly TA. Charles LE, Miller DB. Area under the curve and other summary indicators of repeated waking cortisol measurements. Psychosom Med 69, 651–659, 2007.10.1097/PSY.0b013e31814c405c17766693Search in Google Scholar

Franco GP, Barros AL, Nogueira-Martins LA, Michel JL. Stress influence on genesis, onset and maintenance of cardiovascular diseases: literature review. J Adv Nurs 43,548–554, 2003.10.1046/j.1365-2648.2003.02753.xOpen DOISearch in Google Scholar

Gammoh OS, Al-Smadi A, Al-Awaida W, Badr MM, Qinna NA. Increased salivary nitric oxide and G6PD activity in refugees with anxiety and stress. Stress Health 32, 435–440, 2016a.10.1002/smi.2666Open DOISearch in Google Scholar

Gammoh OS, Al-Smadi AM, Ashour AF, Al-Awaida W. Salivary nitric oxide, a biomarker for stress and anxiety? Psychiatry Investig 13, 311–315, 2016b.10.4306/pi.2016.13.3.311Open DOISearch in Google Scholar

Green, LC, Wagner, DA, Glogowski J, Skipper PL, Wishnok, JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N]nitrate in biological bluids. Anal Biochem 126, 131–138, 1982.10.1016/0003-2697(82)90118-XSearch in Google Scholar

Hlavacova N, Solarikova P, Marko M, Brezina I, Jezova D. Blunted cortisol response to psychosocial stress in atopic patients is associated with decrease in salivary alpha-amylase and aldosterone: Focus on sex and menstrual cycle phase. Psychoneuroendocrinology 78, 31–38, 2017.10.1016/j.psyneuen.2017.01.0072815243128152431Open DOISearch in Google Scholar

Inder WJ, Dimeski G, Russell A. Measurement of salivary cortisol in 2012 - laboratory techniques and clinical indications. Clin Endocrinol (Oxf) 77, 645–651, 2012.10.1111/j.1365-2265.2012.04508.x22812714Search in Google Scholar

Jezova D, Jurankova E, Mosnarova A, Kriska M, Skultetyova I. Neuroendocrine response during stress with relation to gender differences. Acta Neurobiol Exp (Wars) 56, 779–785, 1996.Search in Google Scholar

Lee BH, Lee SW, Yoon D, Lee HJ, Yang JC, Shim SH, Kim DH, Ryu SH, Han C, Kim YK. Increased plasma nitric oxide metabolites in suicide attempters. Neuropsychobiology 53,127–132, 2006.10.1159/00009254216601363Search in Google Scholar

Levine A, Zagoory-Sharon O, Feldman R, Lewis JG, Weller A. Measuring cortisol in human psychobiological studies. Physiol Behav 90, 43–53, 2007.10.1016/j.physbeh.2006.08.0251705500617055006Open DOISearch in Google Scholar

Maidana P, Bruno OD, Mesch V. A critical analysis of cortisol measurements: an update. Medicina (B Aires) 73, 579–584, 2013.Search in Google Scholar

McEwen BS. Physiology and neurobiology of stress and adaptation: central role of the brain. Physiol Rev 87, 873–904, 2007.10.1152/physrev.00041.200617615391Search in Google Scholar

Miller DB, O’Callaghan JP. Neuroendocrine aspects of the response to stress. Metabolism 51, 5–10, 2002.10.1053/meta.2002.331841204053412040534Open DOISearch in Google Scholar

Mukaka MM. A guide to appropriate use of correlation coefficient in medical research. Malawi Med J 24, 69–71, 2012.Search in Google Scholar

Pruessner JC, Kirschbaum C, Meinlschmid G, Hellhammer DH. Two formulas for computation of the area under the curve represent measures of total hormone concentration versus time-dependent change. Psychoneuroendocrinology 28, 916–931, 2003.1289265810.1016/S0306-4530(02)00108-7Search in Google Scholar

Puzserova A, Bernatova I. Blood pressure regulation in stress: focus on nitric oxide-dependent mechanisms. J Physiol 564, 321–327, 2005.Search in Google Scholar

Ross KM, Murphy MLM, Adam EK, Chen E, Miller GE. How stable are diurnal cortisol activity indices in healthy individuals? Evidence from three multi-wave studies. Psychoneuroendocrinology 39, 184–193, 2014.10.1016/j.psyneuen.2013.09.016386964024119668Search in Google Scholar

Silva, RAO, Guillo, LA. Condicoes de trabalho e estresse: um estudo com professores da Educacao Basica. Trabalho & Educacao 24, 153–166, 2015.Search in Google Scholar

Theorell T, Hammarstrom A, Aronsson G, Traskman Bendz L, Grape T, Hogstedt C, Marteinsdottir I, Skoog I, Hall C. A systematic review including meta-analysis of work environment and depressive symptoms. BMC Public Health.15, 738–745, 2015.10.1186/s12889-015-1954-426232123452205826232123Open DOISearch in Google Scholar

Theorell T, Jood K, Jarvholm LS, Vingard E, Perk J, Ostergren PO, Hall C. A systematic review of studies in the contributions of the work environment to ischaemic heart disease development. Eur J Public Health 26, 470–477, 2016.10.1093/eurpub/ckw02527032996488433027032996Open DOISearch in Google Scholar

Toda N, Nakanishi-Toda M. How mental stress affects endothelial function. Pflugers Arch- Eur J Physiol 462, 779–794, 2011.10.1007/s00424-011-1022-621947555Search in Google Scholar

Tsikas D. Methods of quantitative analysis of the nitric oxide metabolites nitrite and nitrate in human biological fluids. Free Radic Res 39, 797–815, 2005.10.1080/1071576050005365116036360Search in Google Scholar

Tsikas D. Analysis of nitrite and nitrate in biological fluids by assays based on the Griess reaction: appraisal of the Griess reaction in the L-arginine/nitric oxide area of research. J Chromatogr B Analyt Technol Biomed Life Sci 15, 51–70, 2007.10.1016/j.jchromb.2006.07.05416950667Search in Google Scholar

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