<abstract xmlns="http://www.w3.org/1999/xhtml">

Lipase activity is a perfect indicator for the monitoring of processes of bioremediation of degraded soils. Lipase is also used in the processes of oil hydrolysis in wastewater treatment. To be able to predict and model processes with used lipase in environmental operations, knowledge of the kinetic parameters of the process are required. The paper presents the determined values of activation energies and optimum temperatures for porcine pancreas lipase. The parameters were estimated based on the literature of the activity curves <italic>vs</italic>. temperature for hydrolysis of olive oil by lipase. It was noticed that concentration of gum arabic added as an emulsifier during lipase activity measurements influences on the obtained values of determined parameters. A mathematical model describing the effect of temperature on porcine pancreas lipase activity was used. Based on the comparison analysis, the optimum temperature <italic>Topt</italic> were obtained in the range from 313.30 ±0.56 to 319.62 ±0.96 K, activation energies <italic>Ea</italic> were from 51 ±10 to 82.6 ±9.9 kJ/mol, and values of deactivation energies <italic>Ed</italic> were in the range from 122.7 ±4.0 to 150.9 ±5.8 kJ/mol.
</abstract>

Lipase activity is a perfect indicator for the monitoring of processes of bioremediation of degraded soils. Lipase is also used in the processes of oil hydrolysis in wastewater treatment. To be able to predict and model processes with used lipase in environmental operations, knowledge of the kinetic parameters of the process are required. The paper presents the determined values of activation energies and optimum temperatures for porcine pancreas lipase. The parameters were estimated based on the literature of the activity curves vs. temperature for hydrolysis of olive oil by lipase. It was noticed that concentration of gum arabic added as an emulsifier during lipase activity measurements influences on the obtained values of determined parameters. A mathematical model describing the effect of temperature on porcine pancreas lipase activity was used. Based on the comparison analysis, the optimum temperature Topt were obtained in the range from 313.30 ±0.56 to 319.62 ±0.96 K, activation energies Ea were from 51 ±10 to 82.6 ±9.9 kJ/mol, and values of deactivation energies Ed were in the range from 122.7 ±4.0 to 150.9 ±5.8 kJ/mol.

The Activation Energies and Optimum Temperatures of Olive Oil Hydrolysis By Lipase Porcine Pancreas

Department of Chemical and Biochemical Engineering, Faculty of Chemical Technology and Engineering

Bydgoszcz University of Science and Technology

Ecological Chemistry and Engineering S

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Lipase activity is a perfect indicator for the monitoring of processes of bioremediation of degraded soils. Lipase is also used in the processes of oil...

The Activation Energies and Optimum Temperatures of Olive Oil Hydrolysis By Lipase Porcine Pancreas

Published Online: Oct 11, 2021

Page range: 389 - 398

DOI: https://doi.org/10.2478/eces-2021-0026

Keywords
lipase porcine pancreas, gum arabic, optimum temperature, activation energy, deactivation energy

© 2021 Justyna Miłek, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Activation Energies and Optimum Temperatures of Olive Oil Hydrolysis By Lipase Porcine Pancreas

Published Online: Oct 11, 2021

Page range: 389 - 398

DOI: https://doi.org/10.2478/eces-2021-0026

Keywordslipase porcine pancreas, gum arabic, optimum temperature, activation energy, deactivation energy

© 2021 Justyna Miłek, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
lipase porcine pancreas, gum arabic, optimum temperature, activation energy, deactivation energy