<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>There is a relationship between a person’s diet and the development and prevention of some cancers. Carotenoids are found as various natural pigments in many fruits and vegetables. Studies on carotenoids and their potential roles in carcinogenesis are increasing in importance day by day. In this study, we aimed to determine the cytotoxic and genotoxic effects of capsanthin, a carotenoid compound, in human prostate cancer cell lines.</p>
<p>After different concentrations of capsanthin were applied to human prostate cancer cell lines (LNCaP and PC-3), the effects of the compound on cell viability were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. The single-cell gel electrophoresis (Comet) assay was then used to reveal the genotoxic effects of probable cytotoxic dosages on cell DNA. After the treatments, apoptotic cell death levels were determined by Tunel staining. At high concentrations, capsanthin dramatically reduced PC-3 and LNCaP cell viability (p&lt;0.05). In addition, capsanthin caused DNA damage and apoptotic cell death in the prostate cancer cells. The results show that capsanthin reduces cell viability by causing genotoxicity in prostate cancer cells.</p>
</abstract>

There is a relationship between a person’s diet and the development and prevention of some cancers. Carotenoids are found as various natural pigments in many fruits and vegetables. Studies on carotenoids and their potential roles in carcinogenesis are increasing in importance day by day. In this study, we aimed to determine the cytotoxic and genotoxic effects of capsanthin, a carotenoid compound, in human prostate cancer cell lines.
After different concentrations of capsanthin were applied to human prostate cancer cell lines (LNCaP and PC-3), the effects of the compound on cell viability were determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. The single-cell gel electrophoresis (Comet) assay was then used to reveal the genotoxic effects of probable cytotoxic dosages on cell DNA. After the treatments, apoptotic cell death levels were determined by Tunel staining. At high concentrations, capsanthin dramatically reduced PC-3 and LNCaP cell viability (p<0.05). In addition, capsanthin caused DNA damage and apoptotic cell death in the prostate cancer cells. The results show that capsanthin reduces cell viability by causing genotoxicity in prostate cancer cells.

Capsanthin induces death in human prostate cancer cell lines by inducing DNA damage

Faculty of Medicine, Department of Physiology

Faculty of Science, Department of Molecular Biology and Genetics

Faculty of Medicine, Department of Biophysics

The EuroBiotech Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

There is a relationship between a person’s diet and the development and prevention of some cancers. Carotenoids are found as various natural pigments in many...

Capsanthin induces death in human prostate cancer cell lines by inducing DNA damage

Published Online: Jul 18, 2022

Page range: 99 - 104

DOI: https://doi.org/10.2478/ebtj-2022-0010

Keywords
Capsanthin, Prostate cancer, Cell Culture, DNA damage, Cell Viability

© 2022 Ersen Eraslan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Capsanthin induces death in human prostate cancer cell lines by inducing DNA damage

Published Online: Jul 18, 2022

Page range: 99 - 104

DOI: https://doi.org/10.2478/ebtj-2022-0010

KeywordsCapsanthin, Prostate cancer, Cell Culture, DNA damage, Cell Viability

© 2022 Ersen Eraslan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Capsanthin, Prostate cancer, Cell Culture, DNA damage, Cell Viability