1. bookVolume 6 (2022): Issue 1 (January 2022)
Journal Details
License
Format
Journal
eISSN
2564-615X
First Published
30 Jan 2017
Publication timeframe
4 times per year
Languages
English
access type Open Access

Serum collected from rats with myocardial infarction increases extracellular matrix accumulation by myofibroblasts isolated from myocardial infarction scar

Published Online: 24 Jan 2022
Page range: 1 - 8
Journal Details
License
Format
Journal
eISSN
2564-615X
First Published
30 Jan 2017
Publication timeframe
4 times per year
Languages
English
Abstract

The effect on extracellular matrix content is believed to be an average of several serum derived compounds acting in opposition. The aim of the study is to determine whether whole serum of rats with myocardial infarction may modify the accumulation of extracellular matrix in cultures of myofibroblasts isolated from the myocardial infarction scar. A second aim is to determine whether the tested serum can also degranulate the mast cells. Serum was collected from rats with sham myocardial infarction, rats with myocardial infarction induced by coronary artery ligation and control animals. The experiments were carried out on myocardial infarction scar myofibroblasts or mast cells from the peritoneal cavity. The cultures were divided into three groups containing eight cultures each: one treated with serum from control rats, from animals after sham operation or from those after myocardial infarction. In all groups, the serum was used at concentrations of 10%, 20% or 30%. The total collagen content (Woesner method) glycosaminoglycan level (Farandale method), cell proliferation (BrdU), histamine secretion from mast cells (spectrofluorymetry), β1 integrin and α-smooth muscle actin expression (flow cytometry) were evaluated. Isolated cells were α-smooth muscle actin positive and identified as myofibroblasts. Serum derived from rats with myocardial infarction increased collagen and glycosaminoglycan content in the cultures and modified myofibroblast proliferation in a concentration-dependent manner. The serum also results in an imbalance between collagen and glycosaminoglycan levels. The content of β1 integrin was not influenced by myocardial infarction serum. The serum of rats with myocardial infarction is involved in regulation of collagen and glycosaminoglycan content in myofibroblast cultures, as well as the modification of their proliferation. These changes were not accompanied with integrin β1 density variations. The serum of the myocardial infarction rats did not influence the mast cell degranulation.

Keywords

1. Fomovsky GM, Thomopoulos S, Holmes JW. Contribution of extracellular matrix to the mechanical properties of the heart. J. Mol. Cell Cardio. 2010; 48(3): 490-496.10.1016/j.yjmcc.2009.08.003282383519686759 Search in Google Scholar

2. Fraccarollolo D, Galuppo P, Bauersachs J, Ertl G. Collagen accumulation aftermyocardial infarction: effects of ETA receptor blockade and implications for early remodeling. Cardiovasc. Res. 2002; 54(3): 559-567. Search in Google Scholar

3. Abaco GM, Kaye AH. Integrins: Molecular determinants of glioma invasion. J. Clin. Neurosci. 2007; 14(11): 1041-1048. Search in Google Scholar

4. Van der Flier A, Sonnenberg A. Fuction and interactions of integrines. Cell Tissue Res. 2001; 305(03): 285-298.10.1007/s00441010041711572082 Search in Google Scholar

5. Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, Parsons JT, Horwitz AR. Cell migration: Integrating signals from front to back. Science. 2003; 302(5651): 1704-1709.10.1126/science.109205314657486 Search in Google Scholar

6. Gałdyszyńska M, Bobrowska E, Lekka M, Radwańska P, Piera L, Szymański J, Drobnik J. The stiffness-controlled release of interleukin-6 by cardiac fibroblasts is dependent on integrin α2β1. J Cell Mol Med. 2020, Dec; 24(23): 13853–13862.10.1111/jcmm.15974775405933124775 Search in Google Scholar

7. Drobnik J, Ciosek J, Jakubowski L. The role of the connective tissue in healing process in myocardial infarction. Kardiologia Polska 2005; 63(4 Suppl.2): 428-439. Search in Google Scholar

8. Dąbrowski R, Drobnik J. The effect of disodium cromoglycate on the skin wound healing and collagen content in the wounds of rats. Acta Physiol. Pol. 1990; 41(4-6): 95-98. Search in Google Scholar

9. Wolak M, Bojanowska E, Staszewska T, Ciosek J, Juszczak M, Drobnik J. The role of histamine in the regulation of the viability, proliferation and Transforming Growth Factor β1 secretion of rat wound fibroblasts. Pharmacol. Rep. 2017; 69: 314-321. Search in Google Scholar

10. Frangogiannis NG, Perrard JL, Mendoza LH, Burns AR, Lindsey ML, Ballantyne CM, Michael LH, Smith CW, Entman ML. Stem cell factor induction is associated with mast cell accumulation with mast cell accumulation after canine myocardial ischemia and reperfusion. Circulation 1998; 98(7): 687-698.10.1161/01.CIR.98.7.687 Search in Google Scholar

11. Akiyama-Uchida Y, Ashizawa N, Ohtsuru A, Seto S, Tsukazaki T, Kikuchi H, Yamashita S, Yano K. Norepinephine enhances fibrosis mediated by TGF-β in cardiac fibro-blasts. Hypertension 2002; 40(2): 148-154.10.1161/01.HYP.0000025443.61926.12 Search in Google Scholar

12. Klett CP, Palmer AA, Dirig DM, Gallagher AM, Riosecco-Camacho N, Printz MP. Evidence for differences in cultured left ventricular fibroblasts populations isolated from spontaneously hypertensive and Wistar-Kyoto rats. J. Hypertens. 1995; 13(12 Pt 1): 1421-1431.10.1097/00004872-199512000-00008 Search in Google Scholar

13. Zhou G, Kandala JC, Tyagi SC, Katwa LC, Weber KTEffects of angiotensin II and aldosterone on collagen gene expression and protein turnover in cardiac fibroblasts. Mol. Cell Biochem. 1996; 154(2): 171-178.10.1007/BF002267858717431 Search in Google Scholar

14. Young M, Fullerton M, Dilley R, Funder J. Mineralocorticoids, hypertension and cardiac fibrosis. J. Clin. Invest. 1994; 93(6): 2578-2583.10.1172/JCI1172692944888200995 Search in Google Scholar

15. Kapoun AM, Liang F, O’Young G, Damm DL, Quon D, White RT, Munson K, Lam A, Shreiner GF, Protter AA. B-type natriuretic peptide exerts broad functional opposition to transforming growth factor-β in primary human cardiac fibroblasts: fibrosis, myofibroblast conversion, proliferation and inflammation. Circ. Res. 2004; 94(4): 453-461. Search in Google Scholar

16. Dubey RK, Gillespie DG, Jackson EK. Adenosine inhibits collagen and protein synthesis in cardiac fibroblasts: Role of A2B receptors. Hypertension 1998; 31(4): 943-948.10.1161/01.HYP.31.4.943 Search in Google Scholar

17. Porter KE, Tuner NA. Cardiac fibroblasts. At the heart of myocardial remodeling. Pharm. Ther. 2009; 123(2): 255-278. Search in Google Scholar

18. Drobnik J, Owczarek K, Piera L, Tosik D, Olczak S, Ciosek J, Hrabec E. Melatonin-induced augmentation of collagen deposition in cultures of fibroblasts and myofibroblasts is blocked by luzindole – melatonin membrane receptors inhibitor. Pharmacol. Rep. 2013; 65(3): 642-649. Search in Google Scholar

19. Piera L, Olczak S, Kun T, Gałdyszyńska M, Ciosek J, Szymański J, Drobnik J. Disruption of histamine/H3receptor signal reduces collagen deposition in cultures scar myofibroblasts. J Physiol Pharmacol 2019; 70(2): 239-247. Search in Google Scholar

20. Farndale RW, Buttle DJ, Barret AJ. Improved quantitation and discrimination of sulphated glycosaminoglycans by use dimethylmethylene blue. Biochem. Biophys. Acta. 1986; 883(2): 173-177. Search in Google Scholar

21. Cheng T, Yue M, Aslam MN, Wang X, Shekhawat G, Varani J, Schuger L. Neuronal Protein 3.1 Deficiency leads to reduced cutaneous scar collagen deposition and tensile strength due to impaired transforming growth factor-β1 to -β3 translation. Am. J. Pathol. 2017; 187(2): 292-303. Search in Google Scholar

22. Cleutiens JP, Verluyten MJ, Smits JF, Daemen M.J. Collagen remodeling after myocardial infarction in the rat heart. Am. J. Pathol. 1995; 147(2): 325-338. Search in Google Scholar

23. Porter KE, Turner NA. Cardiac fibroblasts: at the heart of myocardial remodeling. Pharmacol. Ther. 2009; 123(2): 255-278. Search in Google Scholar

24. Anand IS, Ferrari R, Kalra GS, Wah PL, Poole-Wilson PA, Harris PC. Edema of cardiac origin. Studies of body water and sodium, renal function, hemodynamic indexes and plasma hormones in untreated congestive cardiac failure. Circulation 1989; 80: 299-305.10.1161/01.CIR.80.2.299 Search in Google Scholar

25. Sakai S, Miyauchi T, Kobayashi M, Goto K. Inhibition of myocardial endothelin pathway improves long-term survival in heart failure. Nature 1996; 384(6607): 353-355.10.1038/384353a0 Search in Google Scholar

26. Kayle DM, Lefkovits J, Jennings GL, Bergin P, Broughton A, Esler MD. Adverse consequences of high sympatetic nervous activity in the failing human heart. J. Am. Coll. Cardiol. 1995; 26(5): 1257-1263. Search in Google Scholar

27. Thibault G, Nemer M, Drouin J, Lavigne JP, Ding J, Charbonneau C, Garcia R, Genest J, Jasmin G, Sole M. Ventricles as a major site of atrial natriuretic factor synthesis and release in cardiomyopatic hamsters with heart failure. Circ. Res. 1989; 65(1): 71-82. Search in Google Scholar

28. Kulke M, Geist N, Friedrichs W, Langel W. Molecular dynamics simulations on networks of heparin and collagen. Proteins 2017; 85(6): 1119-1130.10.1002/prot.25277 Search in Google Scholar

29. Chen S, Young MF, Chakravarti S, Birk DE. Interclass small leucine-rich repeat proteoglycan interactions regulate collagen fibrillogenesis and corneal stromal assembly. Matrix Biol. 2014; 35: 103-111.10.1016/j.matbio.2014.01.004 Search in Google Scholar

30. Reese SP, Underwood CJ, Weiss JA. Effects of decorin proteoglycan on fibrillogenesis, ultrastructure, and mechanics of type I collagen gels. Matrix Biol. 2013;32(7-8):414-423.10.1016/j.matbio.2013.04.004 Search in Google Scholar

31. Pietraszek K, Chatron-Colliet A, Brézillon S, Perreau C, Jakubiak-Augustyn A, Krotkiewski H, Maquart FX, Wegrowski Y. Lumican: a new inhibitor of matrix metalloproteinase-14 activity. FEBS Lett. 2014; 588(23): 4319-4324.10.1016/j.febslet.2014.09.040 Search in Google Scholar

32. Drobnik J, Szczepanowska A, Dąbrowski R. Temporary augmentation of glycosaminoglycans content in the heart after left coronary artery ligation. Pathophysiology 2004; 11(1): 35-39.10.1016/j.pathophys.2004.01.024 Search in Google Scholar

33. Dąbrowski R, Maslinski C. The effect of histamine on collage formation and collage polymerisation In the skin wounds healing of guinea pigs. Life Sci. 1970; 9: 189-202.10.1016/0024-3205(70)90068-8 Search in Google Scholar

34. Saito T, Tazawa K, Yokoyama Y, Saito M. Surgical stress inhibits the growth of fibroblasts through the elevation of plasma catecholamine and cortisol concentrations. 1997; 27(7): 627-631. Search in Google Scholar

35. Anitua E, Muruzabal F, de la Fuente M, Riestra A, Merayo-Lloves J, Orive G. PRGF exerts more potent proliferative and anti-inflammatory effects than autologous serum on a cell culture inflammatory model. Exp. Eye Res. 2016; 151: 115-121.10.1016/j.exer.2016.08.01227567559 Search in Google Scholar

36. Leicht M, Briest W, Zimmer HG. Regulation of norepinerine-induced proliferation in cardiac fibroblasts by inter-leukin-6 and p42/p44 mitogen activated protein kinase. Mol. Cell Biochem. 2003; 243: 65-72.10.1023/A:1021655023870 Search in Google Scholar

37. Turner NA, Porter KE, Smith WH, White HL, Ball SG, Balmforth A. Chronic β2-adrenergic receptor stimulation increases proliferation of human cardiac fibroblasts via an autocrine mechanism. Cardiovasc. Res. 2003; 57(3): 784-792. Search in Google Scholar

38. Yang Y, Chen S, Tao L, Gan S, Luo H, Xu Y, Shen X.Inhibitory Effects of Oxymatrine on Transdifferentiation of Neonatal Rat Cardiac Fibroblasts to Myofibroblasts Induced by Aldosterone via Keap1/Nrf2 Signaling Pathways In Vitro. Med Sci Monit. 2019; 25: 5375-5388.10.12659/MSM.915542666294331325292 Search in Google Scholar

39. Janbandhu V, Tallapragada V, Patrick R, Li Y, Abeygunawardena D, Humphreys DT, Martin E, Ward AO, Contreras O, Farbehi N, Yao E, Du J, Dunwoodie SL, Bursac N, Harvey RP. Hif-1a suppresses ROS-induced proliferation of cardiac fibroblasts following myocardial infarction. Cell Stem Cell 2021; S1934-5909(21): 00421-5.10.1016/j.stem.2021.10.009902192734762860 Search in Google Scholar

40. Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC Jr. B-type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts. J Cell Physiol. 2010; 225: 251-5.10.1002/jcp.22253383553520506274 Search in Google Scholar

41. Gu Y, Yang DK, Spinas E, Kritas SK, Saggini A, Caraffa A, Antinolfi P, Saggini R, Conti P. Role of TNF in mast cell neuroinflammation and pain. J. Biol. Regul. Homeost. Agents. 2015; 29(4): 787-791. Search in Google Scholar

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