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In vitro production of sesquiterpenes by electro-elicitation in Gyrinops walla Gaetner calli and cell suspension cultures

D. Sachithri P. Munasinghe
D. Sachithri P. Munasinghe
, 
Seneviratnege Somaratne
Seneviratnege Somaratne
, 
Shyama R. Weerakoon
Shyama R. Weerakoon
 and 
Chandani Ranasinghe
Chandani Ranasinghe
   | Apr 22, 2021
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Article Category: Research Article
Published Online: Apr 22, 2021
Page range: 70 - 78
DOI: https://doi.org/10.2478/ebtj-2021-0011
Keywords
© 2021 D. Sachithri P. Munasinghe, Seneviratnege Somaratne, Shyama R. Weerakoon, Chandani Ranasinghe, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Experiment set up for the electrostimulation for G. walla callus and cell suspension(a) diagram of the electro-elicitation apparatus for calli, (b) attached screw to adjust the distance between the callus and the wire electrode, (c) bottom part with aluminium disk and (d) diagram of electro-elicitation apparatus for cell suspension.
Experiment set up for the electrostimulation for G. walla callus and cell suspension(a) diagram of the electro-elicitation apparatus for calli, (b) attached screw to adjust the distance between the callus and the wire electrode, (c) bottom part with aluminium disk and (d) diagram of electro-elicitation apparatus for cell suspension.

Figure 2

Electro-elicitation apparatus for calli which are electrically coupled in series.
Electro-elicitation apparatus for calli which are electrically coupled in series.

Figure 3

Electro-elicitation apparatus for cell suspension which are electrically coupled in series.
Electro-elicitation apparatus for cell suspension which are electrically coupled in series.

Figure 4

TLC profiles of electro-elicited G. walla(a) calli1) 10 mA, 2) 50 mA on 8th week and (b) cell suspension3) 0.1 mA on 6th hour.
TLC profiles of electro-elicited G. walla(a) calli1) 10 mA, 2) 50 mA on 8th week and (b) cell suspension3) 0.1 mA on 6th hour.

Figure 5

Mean area percentages of sesquiterpenes produced in G. walla calli under electric currents of (a) 10 mA and (b) 50mA.
Mean area percentages of sesquiterpenes produced in G. walla calli under electric currents of (a) 10 mA and (b) 50mA.

Figure 6

Mean area percentages of sesquiterpenes produced in G. walla cell suspension under electric current 0.1 mA
Mean area percentages of sesquiterpenes produced in G. walla cell suspension under electric current 0.1 mA

Figure 7

Trends of area percentages of biologically elicited (a) G.walla calli and (b) cell suspension. Note: the graphs included the data upto maximum area percentage of sesquiterpenes
Trends of area percentages of biologically elicited (a) G.walla calli and (b) cell suspension. Note: the graphs included the data upto maximum area percentage of sesquiterpenes

Effect of electro-elicitation G. walla cell suspension

Intensity Incubation period Area percentage of
γ-selinene β-caryophyllene α-guaiene
Control 0 0.01 (0.005)d 0.01 (0.005)b 0.01 (0.005)d
0.1 0.5 0.01 (0.003)c 0.01 (0.005)b 0.02 (0.001)c
0.1 2 0.01 (0.005)c 0.01 (0.005)b 0.09 (0.01)b
0.1 4 0.02 (0.005)bc 0.04 (0.005)b 0.11 (0.01)b
0.1 6 0.04 (0.005)ab 0.05 (0.001)a 0.16 (0.01)a
0.1 12 0.03 (0.01)a 0.02 (0.01)b 0.10 (0.003)b
0.1 24 0.01 (0.005)d 0.01 (0.005)b 0.08 (0.01)b
0.1 72 0.01(0.005)d 0.01 (0.005)b 0.01 (0.005)d

Effect of electro-elicitation on G. walla calli

Intensity Incubation period (weeks) Area percentage of
Control γ-selinne β-caryophyllene α-cardinol α-guaiene
10 2 0.01 (0.005)e 0.01 (0.01)e 0.04 (0.005)d 0.01 (0.005)g
10 4 0.06 (0.04)ed 0.01 (0.005)e 0.06 (0.02)d 0.07 (0.02)de
10 6 0.11 (0.01)ed 0.07 (0.02)e 0.85 (0.05)b 0.05 (0.03)ef
10 8 0.25 (0.04)bcde 1.33 (0.07)a 0.86 (0.05)a 0.13 (0.04)b
10 10 0.19 (0.01)ed 1.11 (0.04)ab 0.48 (0.03)bc 0.18 (0.01)a
10 12 0.10 (0.01)ed 0.09 (0.01)e 0.07 (0.02)d 0.05 (0.02)de
10 14 0.08 (0.005)ed 0.02 (0.002)e 0.01 (0.004)d 0.01 (0.006)g
50 2 0.01 (0.003)e 0.01 (0.001)e 0.01 (0.005)d 0.01 (0.005)g
50 4 0.03 (0.02)ed 0.85 (0.05)d 0.03 (0.01)d 0.01 (0.005)g
50 6 0.42 (0.03)cb 1.15 (0.05)c 0.52 (0.05)b 0.09 (0.01)cd
50 8 0.46 (0.05)b 1.37 (0.02)bc 0.84 (0.02)a 0.09 (0.005)bc
50 10 0.88 (0.02)bcd 1.04 (0.04)cd 0.48 (0.03)b 0.07 (0.02)de
50 12 0.27 (0.02)a 0.08 (0.02)e 0.16 (0.01)cd 0.02 (0.01)gf
50 14 0.01 (0.005)e 0.01 (0.01)e 0.01 (0.004)d 0.01 (0.006)g
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