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Figure 1
Cancer model cell lines: A) UMUC-3, B) SK-MEL30 C) MCF-7 (Invert, phase contrast light microscope)
Figure 2
Cell viability profiles of the A) UMUC-3 cells, B) SK-MEL-30, C) MCF-7 cells at the selected PDT wavelength conditions. The significantly different groups were demonstrated by asterisk (p<0.05).
Figure 3
Annexin-V/7-AAD apoptosis/necrosis analysis of 30 s and 60 s PDT treated UMUC-3 cells.
Figure 4
Annexin-V/7-AAD apoptosis/necrosis analysis of 30 s and 60 s PDT treated SK-MEL-30 cells.
Figure 5
Annexin-V/7-AAD apoptosis/necrosis analysis of 30 s and 60 s PDT treated MCF-7 cells.
Figure 6
Intracellular fluorescent (PpIX) accumulation induced by ALA treatment observed after PBS washes, (40X-oil, 512x512). A) UMUC-3 cells non-ALA treated, observed by confocal microscope with standard detector-SD; B) UMUC-3 cells non-ALA treated, observed in white field by transmitted detector-TD; C) ALA treated UMUC-3 cells, observed by confocal microscope with SD; D) ALA treated UMUC-3 cells observed by white field and confocal with SD and TD; E) SK-MEL-30 cells non-ALA treated, observed by confocal microscope with SD; F) SK-MEL-30 cells non-ALA treated, observed in white field by TD; G) ALA treated SK-MEL-30 cells, observed by confocal microscope with SD; H) ALA treated SK-MEL-30 cells observed by white field and confocal with SD and TD; I) MCF-7 cells non-ALA treated, observed by confocal microscope with SD; J) MCF-7 cells non-ALA treated, observed in white field by TD; K) ALA treated MCF-7 cells, observed by confocal microscope with SD; L) ALA treated MCF-7 cells, observed by white field and confocal with SD and TD. (Excitation: 405 nm; emission: 614 nm)
Representation of % cell populations that are living and that experience cell death through apoptosis and necrosis after PDT. (* Considerable cell death percentages)