<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Sepsis is a systemic infectious disease that leads to shock, organ failure, and death and requires urgent treatment. Animal model studies of sepsis and endotoxemia have revealed that antioxidant compounds prevent the progression of multi-system organ failure and reduce death rate. In the present study aimed to establish the effect of propolis, which has been proven to have antibacterial, anti-inflammatory and antioxidant activities in recent years, on lipopolysaccharide (LPS)-induced renal damage. 40 <italic>Sprague dawley</italic> rats were randomly divided into five equal groups (n = 8): Control (0.9% NaCl), LPS (30 mg/kg), propolis (250 mg/kg), propolis + LPS, and LPS + propolis. After completion of the experimental protocol, Malondialdehyde (MDA) levels were measured using blood serum samples obtained from the rats. The kidney samples of the rats were examined histopathologically. As a result, it was determined that LPS increased MDA levels in the blood serum samples and it caused histological changes in the kidney tissue such as tubular damage, mild ischemic injury, ischemic damage in the form of vacuolization, tubular epithelial vacuolization, vascular congestion, and glomerular atrophy. Contrary to these results, MDA levels of serum decreased in the propolis + LPS, and LPS + propolis groups, and also histological findings improved. These results showed that protective effect of propolis against kidney damage caused by LPS.</p></abstract>

Sepsis is a systemic infectious disease that leads to shock, organ failure, and death and requires urgent treatment. Animal model studies of sepsis and endotoxemia have revealed that antioxidant compounds prevent the progression of multi-system organ failure and reduce death rate. In the present study aimed to establish the effect of propolis, which has been proven to have antibacterial, anti-inflammatory and antioxidant activities in recent years, on lipopolysaccharide (LPS)-induced renal damage. 40 Sprague dawley rats were randomly divided into five equal groups (n = 8): Control (0.9% NaCl), LPS (30 mg/kg), propolis (250 mg/kg), propolis + LPS, and LPS + propolis. After completion of the experimental protocol, Malondialdehyde (MDA) levels were measured using blood serum samples obtained from the rats. The kidney samples of the rats were examined histopathologically. As a result, it was determined that LPS increased MDA levels in the blood serum samples and it caused histological changes in the kidney tissue such as tubular damage, mild ischemic injury, ischemic damage in the form of vacuolization, tubular epithelial vacuolization, vascular congestion, and glomerular atrophy. Contrary to these results, MDA levels of serum decreased in the propolis + LPS, and LPS + propolis groups, and also histological findings improved. These results showed that protective effect of propolis against kidney damage caused by LPS.

Antioxidative role of propolis on LPS induced renal damage

The EuroBiotech Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Antioxidative role of propolis on LPS induced renal damage"}

Sepsis is a systemic infectious disease that leads to shock, organ failure, and death and requires urgent treatment. Animal model studies of sepsis and...

Compounds	%	Compounds	%
Flavonoids	41,2	Aromatic acids and their esters	27,13
4,5 dimethoxy-(2-propenyl) 2-phenol		Caffeic acid
Pinocembrin		Ferulic acid
Chrysin		Benzoic acid
Galangin		Caffeic acid phenetyhl ester
Organic and fatty acids	12,63	Alcohol, ketone and terpenes	19,04
Decanoic acid		2-propen-1-ol
4-pentenoic acid		5-3,3-dimethyl-cyclohexanone
Cinnamic acid		2-Nonadecanone
3-hydroxy-4-methoxycinnamic acid		Gamma-eudesmol
2-propenoic acid		Beta-eudesmol
3,4-dimethoxycinnamic acid		Alpha-eudesmol
Coumaric acid		Alpha-bisabolol
9-Octadecanoic acid		2-propen-1-one
Octadecanoic acid

Antioxidative role of propolis on LPS induced renal damage

Article Category: Research Article

Published Online: Jul 19, 2020

Page range: 156 - 160

DOI: https://doi.org/10.2478/ebtj-2020-0018

Keywords
Sepsis, Propolis, LPS-induced, MDA Levels, Rat

© 2020 Züleyha Doğanyiğit, Birkan Yakan, Aslı Okan, Sibel Silici, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Figure 1

Figure 2

Chemical content of propolis used in this study (GC-MS)

Antioxidative role of propolis on LPS induced renal damage

Article Category: Research Article

Published Online: Jul 19, 2020

Page range: 156 - 160

DOI: https://doi.org/10.2478/ebtj-2020-0018

KeywordsSepsis, Propolis, LPS-induced, MDA Levels, Rat

© 2020 Züleyha Doğanyiğit, Birkan Yakan, Aslı Okan, Sibel Silici, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Figure 1

Figure 2

Chemical content of propolis used in this study (GC-MS)

Keywords
Sepsis, Propolis, LPS-induced, MDA Levels, Rat