<abstract xmlns="http://www.w3.org/1999/xhtml">To develop tomato hybrids and varieties with a high fruit quality, we gradually solved the following tasks: development of DNA-marking methods for long shelf life genes; the genes modifying the biosynthesis of carotenoids and their composition; testing of the elaborated methods on the developed breeding material; the selection of samples with different allelic composition of fruit quality genes; the development of F<bold>1</bold> hybrids using the method of successive crosses and their study; the selection of tomato forms by DNA-typing methods with target genes in F<bold>2</bold> populations to develop valuable breeding samples; the study of carotenoids’ accumulation peculiarities and their inheritance.We used DNA-identification methods for fruit quality genes: <italic>nor, rin</italic>, <italic>norA</italic> (long shelf life), <italic>B, og<bold>c</bold></italic>, <italic>hp2<bold>dg</bold>, gf-3</italic> (carotenoid content). The tomato hybrids, combining two pigment content genes and one long shelf life gene and the model forms with different combinations of fruit quality genes (<italic>B/rin/gf-3, B/rin/hp2<bold>dg</bold>; B <bold>/</bold>nor/gf-3, B <bold>/</bold>nor/hp2<bold>dg</bold>; оg<bold>c /</bold>rin/gf-3, оg<bold>c /</bold>rin/hp2<bold>dg</bold>; оg<bold>c /</bold>nor/gf-3, оg<bold>c /</bold>nor/hp2<bold>dg</bold></italic>) in a homozygous state were developed.Use of the developed accessions with carotenoid content genes (<italic>og<bold>c</bold>/hp2<bold>dg</bold>, B/hp2<bold>dg</bold>)</italic> as maternal forms and the accessions with complex fruit quality genes (<italic>og<bold>c</bold>/hp2<bold>dg</bold>/nor, ogc/hp2<bold>dg</bold>/rin, B/hp2<bold>dg</bold>/nor</italic>, <italic>B/hp2<bold>dg</bold>/rin, og<bold>c</bold>/gf-3/nor</italic>, <italic>og<bold>c</bold>/gf-3/rin</italic>, <italic>B/gf-3/rin, B/gf-3/nor</italic>) as paternal forms for hybridization contributes to high accumulation of carotenoids and a lond period of fruit storability.</abstract>

To develop tomato hybrids and varieties with a high fruit quality, we gradually solved the following tasks: development of DNA-marking methods for long shelf life genes; the genes modifying the biosynthesis of carotenoids and their composition; testing of the elaborated methods on the developed breeding material; the selection of samples with different allelic composition of fruit quality genes; the development of F1 hybrids using the method of successive crosses and their study; the selection of tomato forms by DNA-typing methods with target genes in F2 populations to develop valuable breeding samples; the study of carotenoids’ accumulation peculiarities and their inheritance.We used DNA-identification methods for fruit quality genes: nor, rin, norA (long shelf life), B, ogc, hp2dg, gf-3 (carotenoid content). The tomato hybrids, combining two pigment content genes and one long shelf life gene and the model forms with different combinations of fruit quality genes (B/rin/gf-3, B/rin/hp2dg; B /nor/gf-3, B /nor/hp2dg; оgc /rin/gf-3, оgc /rin/hp2dg; оgc /nor/gf-3, оgc /nor/hp2dg) in a homozygous state were developed.Use of the developed accessions with carotenoid content genes (ogc/hp2dg, B/hp2dg) as maternal forms and the accessions with complex fruit quality genes (ogc/hp2dg/nor, ogc/hp2dg/rin, B/hp2dg/nor, B/hp2dg/rin, ogc/gf-3/nor, ogc/gf-3/rin, B/gf-3/rin, B/gf-3/nor) as paternal forms for hybridization contributes to high accumulation of carotenoids and a lond period of fruit storability.

Genetic bases of tomatо marker-assisted selection in Belarus

Laboratory of Ecological Genetics and Biotechnology

the Institute of Genetics and Cytology, NAS of Belarus

The EuroBiotech Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Genetic bases of tomatо marker-assisted selection in Belarus"}

To develop tomato hybrids and varieties with a high fruit quality, we gradually solved the following tasks: development of DNA-marking methods for long shelf...

Genetic bases of tomatо marker-assisted selection in Belarus

Article Category: Research Article

Published Online: Apr 25, 2018

Page range: 128 - 135

DOI: https://doi.org/10.2478/ebtj-2018-0017

Keywords
tomato, DNA-marker, fruit quality breeding

© 2018 Olga G. Babak, Natalia A. Nekrashevich, Konstantsia K. Yatsevich, Sergey V. Мalyshev, Alexander V. Kilchevsky, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genetic bases of tomatо marker-assisted selection in Belarus

Article Category: Research Article

Published Online: Apr 25, 2018

Page range: 128 - 135

DOI: https://doi.org/10.2478/ebtj-2018-0017

Keywordstomato, DNA-marker, fruit quality breeding

© 2018 Olga G. Babak, Natalia A. Nekrashevich, Konstantsia K. Yatsevich, Sergey V. Мalyshev, Alexander V. Kilchevsky, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
tomato, DNA-marker, fruit quality breeding