<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A High Performance Ion Chromatographic method for the qualitative and quantitative determination of low molecular weight carbohydrates in tobacco leaf is described. The carbohydrates were separated by running isocratic or gradient elutions on columns packed with amino-modified polystyrene divinylbenzene phases, using 0.1 N NaOH as eluent. The compounds were detected by a Pulsed Amperometric Detector, after post-column addition of 1 N NaOH solution. Twenty-two and twenty-four carbohydrates out of twenty-five were separated by isocratic and gradient elution, respectively. Some mono and disaccharides were identified in tobacco samples by their retention times and some quantitative determinations of glucose, fructose, inositol and saccharose were carried out using different varieties of tobacco.</p></abstract>

A High Performance Ion Chromatographic method for the qualitative and quantitative determination of low molecular weight carbohydrates in tobacco leaf is described. The carbohydrates were separated by running isocratic or gradient elutions on columns packed with amino-modified polystyrene divinylbenzene phases, using 0.1 N NaOH as eluent. The compounds were detected by a Pulsed Amperometric Detector, after post-column addition of 1 N NaOH solution. Twenty-two and twenty-four carbohydrates out of twenty-five were separated by isocratic and gradient elution, respectively. Some mono and disaccharides were identified in tobacco samples by their retention times and some quantitative determinations of glucose, fructose, inositol and saccharose were carried out using different varieties of tobacco.

Determination of 25 Low Molecular Weight Carbohydrates in Tobacco by High Performance Ion Chromatography

DivisioneRicerche, Monopoli di Stato, Piazza Mastai 11, I-00153 Roma, Italy

Contributions to Tobacco & Nicotine Research

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A High Performance Ion Chromatographic method for the qualitative and quantitative determination of low molecular weight carbohydrates in tobacco leaf is...

Determination of 25 Low Molecular Weight Carbohydrates in Tobacco by High Performance Ion Chromatography

Published Online: Jan 06, 2015

Page range: 77 - 84

Received: Mar 25, 1993

Accepted: Jan 04, 1994

DOI: https://doi.org/10.2478/cttr-2013-0634

© 1994 Beiträge zur Tabakforschung International

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.