<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A quantitative method for the determination of benz[a]anthracene (B[a]A) and benzo[a]pyrene (B[a]P) in mainstream and sidestream cigarette smoke by high performance liquid chromatography (HPLC) has been developed. Mainstream and sidestream particulate matter is collected on Cambridge filter pads. The polycyclic aromatic hydrocarbons (PAH) are extracted with cyclohexane and subjected to normal-phase chromatography to isolate target fractions which are concentrated and then diluted with acetonitrile or acetonitrile/water prior to reverse-phase analytical chromatography. The Kentucky Reference cigarette 1R4F and a cigarette which heats but does not burn tobacco (New Cigarette) developed at R.J. Reynolds Tobacco Company were analysed and compared with respect to B[a]P and B[a]A in both mainstream and sidestream smoke.</p></abstract>

A quantitative method for the determination of benz[a]anthracene (B[a]A) and benzo[a]pyrene (B[a]P) in mainstream and sidestream cigarette smoke by high performance liquid chromatography (HPLC) has been developed. Mainstream and sidestream particulate matter is collected on Cambridge filter pads. The polycyclic aromatic hydrocarbons (PAH) are extracted with cyclohexane and subjected to normal-phase chromatography to isolate target fractions which are concentrated and then diluted with acetonitrile or acetonitrile/water prior to reverse-phase analytical chromatography. The Kentucky Reference cigarette 1R4F and a cigarette which heats but does not burn tobacco (New Cigarette) developed at R.J. Reynolds Tobacco Company were analysed and compared with respect to B[a]P and B[a]A in both mainstream and sidestream smoke.

The Determination of Benzo[a]Pyrene and Benz[a]Anthracene in Mainstream and Sidestream Smoke of the Kentucky Reference Cigarette 1R4F and a Cigarette Which Heats but Does Not Burn Tobacco: A Comparison

R.J. Reynolds Tobacco Company, Bowman Gray Technical Center, Winston-Salem, North Carolina, U.S.A.

Contributions to Tobacco & Nicotine Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

A quantitative method for the determination of benz[a]anthracene (B[a]A) and benzo[a]pyrene (B[a]P) in mainstream and sidestream cigarette smoke by high...

The Determination of Benzo[a]Pyrene and Benz[a]Anthracene in Mainstream and Sidestream Smoke of the Kentucky Reference Cigarette 1R4F and a Cigarette Which Heats but Does Not Burn Tobacco: A Comparison

Published Online: Jan 06, 2015

Page range: 11 - 17

Received: Apr 13, 1989

Accepted: Sep 06, 1989

DOI: https://doi.org/10.2478/cttr-2013-0617

© 1991 Beiträge zur Tabakforschung International

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.