<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Three fungal strains isolated from tobacco were cultured on tobacco water extracts. In these cultures, the mycelium weight was shown to be correlated with the concentration of a steroid, ergosta-5,7,22-trien-3ss-ol [ergosterol]. This steroid is not a tobacco constituent, but tobacco samples where mold or yeast infections had occurred exhibited significant amounts of it. A method is proposed to quantify ergosterol in tobacco samples by reverse-phase high-performance liquid chromatography (HPLC) with UV detection at 282 nm. 7-Dehydrocholesterol can be used as internal standard. When found in a tobacco sample, the ergosterol concentration exhibits a good correlation with that of another related steroid, ergosta-4,6,8(14),22-tetraen-3-one [ETO], for which an HPLC quantification method in tobacco is proposed. Because it is highly fluorescent, ETO is also amenable to a sensitive and quick determination by thin-layer chromatography (TLC). Once produced on tobacco, ergosterol concentration remains stable through storage under normal conditions, and even primary processing does not alter it appreciably. Possible applications of ergosterol analysis to the detection of fungal infections or the monitoring of fungal growth on tobacco are outlined. In addition, TLC estimation of the ETO concentration in a sample may constitute a convenient and fast screening method for fungal infections.</p></abstract>

Three fungal strains isolated from tobacco were cultured on tobacco water extracts. In these cultures, the mycelium weight was shown to be correlated with the concentration of a steroid, ergosta-5,7,22-trien-3ss-ol [ergosterol]. This steroid is not a tobacco constituent, but tobacco samples where mold or yeast infections had occurred exhibited significant amounts of it. A method is proposed to quantify ergosterol in tobacco samples by reverse-phase high-performance liquid chromatography (HPLC) with UV detection at 282 nm. 7-Dehydrocholesterol can be used as internal standard. When found in a tobacco sample, the ergosterol concentration exhibits a good correlation with that of another related steroid, ergosta-4,6,8(14),22-tetraen-3-one [ETO], for which an HPLC quantification method in tobacco is proposed. Because it is highly fluorescent, ETO is also amenable to a sensitive and quick determination by thin-layer chromatography (TLC). Once produced on tobacco, ergosterol concentration remains stable through storage under normal conditions, and even primary processing does not alter it appreciably. Possible applications of ergosterol analysis to the detection of fungal infections or the monitoring of fungal growth on tobacco are outlined. In addition, TLC estimation of the ETO concentration in a sample may constitute a convenient and fast screening method for fungal infections.

Evaluation of Selected Steroids as Chemical Markers of Past or Presently Occurring Fungal Infections on Tobacco

Philip Morris Europe, Research Division, Neuchatel, Switzerland

Contributions to Tobacco & Nicotine Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Three fungal strains isolated from tobacco were cultured on tobacco water extracts. In these cultures, the mycelium weight was shown to be correlated with the...

Evaluation of Selected Steroids as Chemical Markers of Past or Presently Occurring Fungal Infections on Tobacco

Published Online: Jan 06, 2015

Page range: 127 - 134

Received: Aug 25, 1987

Accepted: Apr 05, 1988

DOI: https://doi.org/10.2478/cttr-2013-0592

© 1988 Beiträge zur Tabakforschung International

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.