<abstract xmlns="http://www.w3.org/1999/xhtml"> Two hundred thirty-eight ground samples of tobacco were scanned with a computerized near-infra-red (NIR) spectrophotometer to study the relationship of NIR spectra to the polyphenol content of the samples. A multiple linear regression model was used to select the most appropriate wavelengths for making the measurements. The equation <inline-formula><alternatives><inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/Untitled-1.jpg"></inline-graphic></alternatives></inline-formula> is a valid equation for predicting polyphenols in tobacco. If the coefficients are validated on the same kind of tobacco and in the same year, the standard error of prediction of the NIR method (± 0.747 %) approaches that of the wet chemistry method (± 0.70 %) with coefficients of variation of 21.75 % and 23.0 %, respectively. The largest standard error of prediction across years was ± 1.353 %. P = polyphenols in % D12 = 2nd derivative parameter i = wavelength in micrometers at which the parameter is measured. </abstract>

Two hundred thirty-eight ground samples of tobacco were scanned with a computerized near-infra-red (NIR) spectrophotometer to study the relationship of NIR spectra to the polyphenol content of the samples. A multiple linear regression model was used to select the most appropriate wavelengths for making the measurements. The equation  is a valid equation for predicting polyphenols in tobacco. If the coefficients are validated on the same kind of tobacco and in the same year, the standard error of prediction of the NIR method (± 0.747 %) approaches that of the wet chemistry method (± 0.70 %) with coefficients of variation of 21.75 % and 23.0 %, respectively. The largest standard error of prediction across years was ± 1.353 %. P = polyphenols in % D12 = 2nd derivative parameter i = wavelength in micrometers at which the parameter is measured.

Rapid Spectrophotometric Analysis of the Chemical Composition of Tobacco: Part 3: Polyphenols

Department of Biological and Agricultural Engineering, North Carolina State University, Raleigh, North Carolina, U.S.A.

Tobacco Research Laboratory, Agricultural Research Service, United States Department of Agriculture, Oxford, North Carolina, U.S.A.

Contributions to Tobacco & Nicotine Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Two hundred thirty-eight ground samples of tobacco were scanned with a computerized near-infra-red (NIR) spectrophotometer to study the relationship of NIR...

Rapid Spectrophotometric Analysis of the Chemical Composition of Tobacco: Part 3: Polyphenols

Published Online: Aug 14, 2014

Page range: 219 - 227

Received: Oct 17, 1980

Accepted: Dec 14, 1981

DOI: https://doi.org/10.2478/cttr-2013-0516

© Beiträge zur Tabakforschung International

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.