<abstract xmlns="http://www.w3.org/1999/xhtml"> This paper illustrates the potential of a computerized spectrophotometer for measuring total alkaloids in tobacco. Prediction equations were developed for three optical parameters. Of the three parameters investigated d2 (log (1/R)) /d/2 gave the best results and d R / R d l gave better results than log (1/R), where reflectance R is the ratio of the detector signal of the sample to the detector signal of the ceramic standard in the reflectance mode. The coefficient of determination r2 for the prediction equation containing d2 (log (1/R)) /d/2 terms at 10 different wavelengths was 0.975. This equation predicted the total alkaloids in an independent set of samples with a standard error of 0.438 %. Instrument noise contributed 43 % of the variation, the remainder was attributed to anomalies of the chemical methods.</abstract>

This paper illustrates the potential of a computerized spectrophotometer for measuring total alkaloids in tobacco. Prediction equations were developed for three optical parameters. Of the three parameters investigated d2 (log (1/R)) /d/2 gave the best results and d R / R d l gave better results than log (1/R), where reflectance R is the ratio of the detector signal of the sample to the detector signal of the ceramic standard in the reflectance mode. The coefficient of determination r2 for the prediction equation containing d2 (log (1/R)) /d/2 terms at 10 different wavelengths was 0.975. This equation predicted the total alkaloids in an independent set of samples with a standard error of 0.438 %. Instrument noise contributed 43 % of the variation, the remainder was attributed to anomalies of the chemical methods.

Rapid Spectrophotometric Analysis of the Chemical Composition of Tobacco: Part 2: TotaI AIkaloids

North Carolina State University, Department of Biological and Agricultural Engineering, Raleigh, North Carolina, U.S.A.

Contributions to Tobacco & Nicotine Research

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This paper illustrates the potential of a computerized spectrophotometer for measuring total alkaloids in tobacco. Prediction equations were developed for...

Rapid Spectrophotometric Analysis of the Chemical Composition of Tobacco: Part 2: TotaI AIkaloids

Published Online: Aug 14, 2014

Page range: 267 - 274

Received: Feb 09, 1978

DOI: https://doi.org/10.2478/cttr-2013-0456

© Beiträge zur Tabakforschung International

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.