<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Various factors which might contribute to the presence of or reflect on the formation of N'-nitrosonornicotine (NNN) or related nitrosamines in tobacco were studied. Using high-performance liquid chromatography (HPLC), the E- and Z-isomers of NNN were clearly separated and the rates of interconversion were determined. The E/Z ratio in tobacco approximated that observed in solution at similar pH and temperature. The influence of curing and stalk position on NNN levels in tobacco was determined. NNN was not detected in green tobacco but was detected in air-cured leaves from the same crop. No significant correlation was observed among stalk position and NNN levels in one variety of bright tobacco. Since NNN may derive from nicotine, two new nitrosamines, a nitrosamino ketone and a nitrosamino aldehyde, which could theoretically arise from nicotine, were synthesized. Analysis of tobacco for these components is currently in progress</p></abstract>

Various factors which might contribute to the presence of or reflect on the formation of N'-nitrosonornicotine (NNN) or related nitrosamines in tobacco were studied. Using high-performance liquid chromatography (HPLC), the E- and Z-isomers of NNN were clearly separated and the rates of interconversion were determined. The E/Z ratio in tobacco approximated that observed in solution at similar pH and temperature. The influence of curing and stalk position on NNN levels in tobacco was determined. NNN was not detected in green tobacco but was detected in air-cured leaves from the same crop. No significant correlation was observed among stalk position and NNN levels in one variety of bright tobacco. Since NNN may derive from nicotine, two new nitrosamines, a nitrosamino ketone and a nitrosamino aldehyde, which could theoretically arise from nicotine, were synthesized. Analysis of tobacco for these components is currently in progress

Chemical Studies on Tobacco Smoke: LI: Studies on Non-Volatile Nitrosamines in Tobacco

Naylor Dana Institute for Disease Prevention, American Health Foundation, Valhalla, New York, U.S.A.

Tobacco Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, Md., U.S.A.

Contributions to Tobacco & Nicotine Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Various factors which might contribute to the presence of or reflect on the formation of N'-nitrosonornicotine (NNN) or related nitrosamines in tobacco were...

Chemical Studies on Tobacco Smoke: LI: Studies on Non-Volatile Nitrosamines in Tobacco

Published Online: Aug 14, 2014

Page range: 1 - 6

DOI: https://doi.org/10.2478/cttr-2013-0419

© Beiträge zur Tabakforschung International

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.