<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> Cigarette smoke condensate (CSC) was fractionated for bioassay to determine possible tumorigenic activity on mouse skin. Two fractions which previously had shown activity were further separated. A weak-acid fraction (F 8) was separated into three subfractions. A polynuclear aromatic hydrocarboncontaining fraction (F 20) was divided into two fractions by gel permeation chromatography. The polynuclear aromatic hydrocarbons, the suspected active materials in F 20, were successfully concentrated into a fraction (F 55) representing only 0.05 % of CSC. These materials are currently undergoing bioassay. </p>
</abstract>

Cigarette smoke condensate (CSC) was fractionated for bioassay to determine possible tumorigenic activity on mouse skin. Two fractions which previously had shown activity were further separated. A weak-acid fraction (F 8) was separated into three subfractions. A polynuclear aromatic hydrocarboncontaining fraction (F 20) was divided into two fractions by gel permeation chromatography. The polynuclear aromatic hydrocarbons, the suspected active materials in F 20, were successfully concentrated into a fraction (F 55) representing only 0.05 % of CSC. These materials are currently undergoing bioassay.

Fractionation of Cigarette Smoke Condensate for Biological Testing: Concentration of Polynuclear Aromatic Hydrocarbon and Weak-Acid Fractions

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Cigarette smoke condensate (CSC) was fractionated for bioassay to determine possible tumorigenic activity on mouse skin. Two fractions which previously had...

Fractionation of Cigarette Smoke Condensate for Biological Testing: Concentration of Polynuclear Aromatic Hydrocarbon and Weak-Acid Fractions

Published Online: Aug 14, 2014

Page range: 132 - 135

Received: Nov 28, 1974

DOI: https://doi.org/10.2478/cttr-2013-0368

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This work is licensed under the Creative Commons Attribution-ShareAlike 4.0 International License.