Nile tilapia Oreochromis niloticus is one of the most important fishery resources and a valuable fish species for aquaculture programmes. It is found in almost all waters and is widely dispersed. Several natural populations of this species have been impacted by genetic pollution despite their significant economic relevance. Understanding population structure is a crucial first step in protecting this species in its native habitats as well as in choosing which wild stocks to use in hatchery initiatives. To demonstrate the genetic-population structure of this species, genetic differences among three geographically secluded populations of O. niloticus were investigated utilizing mitochondrial DNA cytochrome b gene sequences. The results were used to estimate the levels of genetic variability within and among the populations. The 56 cyt b (821 bp) sequences analysis revealed 21 haplotypes, with a nucleotide diversity of 0.0510 and a haplotype diversity of 0.881. In each of the populations, seven (7) singleton variable sites and 19 informative-parsimony sites, genetic diversity could be identified and few population haplotypes were found, indicating a minor genetic distinction between them. For the purpose of conservation and/or Nile tilapia breeding programmes, this information would assist in choosing the fish populations that maintain greater genetic variation in O. niloticus.
