<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The population structure and genetic variability of North African catfish <italic>Clarias gariepinus</italic> (Burchell 1822) were investigated using partial mitochondrial DNA cytochrome b region sequences. Fifty-four (54) samples were investigated from three geographically isolated rivers in Nigeria. The analysis of 53 haplotypes revealed greater haplotype diversity (0.99930) and nucleotide diversity (p) (0.07270). According to an analysis of molecular variance (AMOVA), the genetic diversity of North African catfish within populations is significantly higher than the genetic diversity across populations. The FST scores (0.75000, 0.94792 and 0.95699) indicated that North African catfish populations in three Nigerian freshwater bodies had a strong genetic structure. The phylogenetic reconstruction of unique haplotypes revealed the placement of a haplotype (Ogbese) linked by others from all three groups with a point mutation ranging from 1 to 24 nucleotides. North African catfish populations in the Asejire and Ureje are genetically diverse, as evidenced by a high level of haplotype diversity of 1.0000, low nucleotide diversity spanning from 0.05101 to 0.07889, and high FST values (within-population genetic variation). The common haplotypes between some populations and mixes of haplotypes from different populations within the same genetic cluster demonstrate that the population genetic structure is not distinct.</p>
</abstract>

The population structure and genetic variability of North African catfish Clarias gariepinus (Burchell 1822) were investigated using partial mitochondrial DNA cytochrome b region sequences. Fifty-four (54) samples were investigated from three geographically isolated rivers in Nigeria. The analysis of 53 haplotypes revealed greater haplotype diversity (0.99930) and nucleotide diversity (p) (0.07270). According to an analysis of molecular variance (AMOVA), the genetic diversity of North African catfish within populations is significantly higher than the genetic diversity across populations. The FST scores (0.75000, 0.94792 and 0.95699) indicated that North African catfish populations in three Nigerian freshwater bodies had a strong genetic structure. The phylogenetic reconstruction of unique haplotypes revealed the placement of a haplotype (Ogbese) linked by others from all three groups with a point mutation ranging from 1 to 24 nucleotides. North African catfish populations in the Asejire and Ureje are genetically diverse, as evidenced by a high level of haplotype diversity of 1.0000, low nucleotide diversity spanning from 0.05101 to 0.07889, and high FST values (within-population genetic variation). The common haplotypes between some populations and mixes of haplotypes from different populations within the same genetic cluster demonstrate that the population genetic structure is not distinct.

Genetic Differentiation and Molecular Phylogenetics of North African Catfish from Three Distinct Waterbodies

Department of Fisheries and Aquaculture Technology

Croatian Journal of Fisheries

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The population structure and genetic variability of North African catfish Clarias gariepinus (Burchell 1822) were investigated using partial mitochondrial DNA...

Genetic Differentiation and Molecular Phylogenetics of North African Catfish from Three Distinct Waterbodies

Published Online: Sep 22, 2022

Page range: 123 - 132

Received: Mar 09, 2022

Accepted: May 27, 2022

DOI: https://doi.org/10.2478/cjf-2022-0013

Keywords
Cytochrome, Median-joining network, Nucleotide substitution, Genetic distances

© 2022 Omoniyi Michael Popoola, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genetic Differentiation and Molecular Phylogenetics of North African Catfish from Three Distinct Waterbodies

Published Online: Sep 22, 2022

Page range: 123 - 132

Received: Mar 09, 2022

Accepted: May 27, 2022

DOI: https://doi.org/10.2478/cjf-2022-0013

KeywordsCytochrome, Median-joining network, Nucleotide substitution, Genetic distances

© 2022 Omoniyi Michael Popoola, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Cytochrome, Median-joining network, Nucleotide substitution, Genetic distances