<abstract xmlns="http://www.w3.org/1999/xhtml">

The study has the intent of exploring the dynamics of biologically active substance (BAS) extractions from red raspberry (<italic>R. idaeus</italic>) shoots in order to determine the appropriate extraction frequency. A secondary objective is to assess the relationship between extract antioxidant activity and BAS content.
The focus of the study revolved around <italic>R. idaeus</italic> shoots, and the spectrophotometric method of analysis was applied to determine the amount of the catechins, polyphenols, hydroxycinnamic acids derivatives and flavonoids, while the gravimetric method was used to determine the dry residue of the extracts and the potentiometric method applied for evaluating antioxidant activity.
The polyphenols amount was 1.74±0.03, 0.58±0.01 and 0.016±0.005%, catechins – 1.50±0.02, 0.50±0.01 and 0.11±0.01%, flavonoids – 0.08±0.005, 0.046±0.005 and 0.016±0.002%, hydroxycinnamic acids derivatives – 0.24±0.005, 0.078±0.005 and 0.017±0.002%, organic acids – 0.15±0.005, 0.05±0.005 and 0.01±0.002, dry residue – 2.14±0.005, 0.72±0.01 and 0.16±0.005%, antioxidant activity – 94.80±1.90, 47.40±0.95 and 7.90±0.16 mM-eqv./m for the 1st, 2nd and 3rd extraction, respectively. The optimal extraction frequency of raspberry shoots with distilled water was two times. The highest value of Peasorn‘s correlation coefficient was between the antioxidant activity and catechins (r = 0.9813) and the lowest in the case of organic acids (r=0.9112).
The appropriate extraction frequency of <italic>R. idaeus</italic> shoots with distilled water was two times. The antioxidant method of determination extraction frequency was not inferior to the traditional method. The catechins dominated in the extracts from <italic>R. idaeus</italic> shoots. A significant positive correlation was content of catechins and antioxidant activity.
</abstract>

The study has the intent of exploring the dynamics of biologically active substance (BAS) extractions from red raspberry (R. idaeus) shoots in order to determine the appropriate extraction frequency. A secondary objective is to assess the relationship between extract antioxidant activity and BAS content.
The focus of the study revolved around R. idaeus shoots, and the spectrophotometric method of analysis was applied to determine the amount of the catechins, polyphenols, hydroxycinnamic acids derivatives and flavonoids, while the gravimetric method was used to determine the dry residue of the extracts and the potentiometric method applied for evaluating antioxidant activity.
The polyphenols amount was 1.74±0.03, 0.58±0.01 and 0.016±0.005%, catechins – 1.50±0.02, 0.50±0.01 and 0.11±0.01%, flavonoids – 0.08±0.005, 0.046±0.005 and 0.016±0.002%, hydroxycinnamic acids derivatives – 0.24±0.005, 0.078±0.005 and 0.017±0.002%, organic acids – 0.15±0.005, 0.05±0.005 and 0.01±0.002, dry residue – 2.14±0.005, 0.72±0.01 and 0.16±0.005%, antioxidant activity – 94.80±1.90, 47.40±0.95 and 7.90±0.16 mM-eqv./m for the 1st, 2nd and 3rd extraction, respectively. The optimal extraction frequency of raspberry shoots with distilled water was two times. The highest value of Peasorn‘s correlation coefficient was between the antioxidant activity and catechins (r = 0.9813) and the lowest in the case of organic acids (r=0.9112).
The appropriate extraction frequency of R. idaeus shoots with distilled water was two times. The antioxidant method of determination extraction frequency was not inferior to the traditional method. The catechins dominated in the extracts from R. idaeus shoots. A significant positive correlation was content of catechins and antioxidant activity.

Investigation of the extraction dynamic of the biologically active substances of the raspberry (<italic>Rubus idaeus</italic> L.) shoots

Department of Analytical Chemistry and Analytical Toxicology

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Investigation of the extraction dynamic of the biologically active substances of the raspberry ( L.) shoots

The study has the intent of exploring the dynamics of biologically active substance (BAS) extractions from red raspberry (R. idaeus) shoots in order to...

Investigation of the extraction dynamic of the biologically active substances of the raspberry (Rubus idaeus L.) shoots

Published Online: Dec 25, 2023

Page range: 194 - 198

Received: Feb 16, 2023

Accepted: Aug 29, 2023

DOI: https://doi.org/10.2478/cipms-2023-0034

Keywords
extraction dynamic, shoots, antioxidant activity, total phenolic content, correlation

© 2023 Olexander Maslov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Investigation of the extraction dynamic of the biologically active substances of the raspberry (Rubus idaeus L.) shoots

Published Online: Dec 25, 2023

Page range: 194 - 198

Received: Feb 16, 2023

Accepted: Aug 29, 2023

DOI: https://doi.org/10.2478/cipms-2023-0034

Keywordsextraction dynamic, shoots, antioxidant activity, total phenolic content, correlation

© 2023 Olexander Maslov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
extraction dynamic, shoots, antioxidant activity, total phenolic content, correlation