<abstract xmlns="http://www.w3.org/1999/xhtml">

Propolis has been reported to have a broad spectrum of biological activities. The objective of this study was to investigate the hepatoprotective effect of propolis on liver injury induced by carbon tetrachloride (CCl4). Twenty-five adult mice were randomly divided into five groups of five. The normal group received distilled water; positive control group was given CCl4 at 2.8 mL/kg BW orally, other groups were given the same dose of CCl4, followed by oral propolis at 25, 50, and 100 mg/kg BW, respectively, for 14 days. On the 15th day, the mice were sacrificed for the measurements of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein, and albumin, as well as examination of liver histology. The data were analyzed using a one-way ANOVA and, subsequently, by Duncan’s Multiple Range Test. The results demonstrated that propolis had a hepatoprotective effect as shown by significant improvement of the biochemical parameters (p &lt;0.05), which was confirmed by the liver histological analytical results. The most potent hepatoprotective effect was found after the dose of 100 mg/kg BW.
</abstract>

Propolis has been reported to have a broad spectrum of biological activities. The objective of this study was to investigate the hepatoprotective effect of propolis on liver injury induced by carbon tetrachloride (CCl4). Twenty-five adult mice were randomly divided into five groups of five. The normal group received distilled water; positive control group was given CCl4 at 2.8 mL/kg BW orally, other groups were given the same dose of CCl4, followed by oral propolis at 25, 50, and 100 mg/kg BW, respectively, for 14 days. On the 15th day, the mice were sacrificed for the measurements of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein, and albumin, as well as examination of liver histology. The data were analyzed using a one-way ANOVA and, subsequently, by Duncan’s Multiple Range Test. The results demonstrated that propolis had a hepatoprotective effect as shown by significant improvement of the biochemical parameters (p <0.05), which was confirmed by the liver histological analytical results. The most potent hepatoprotective effect was found after the dose of 100 mg/kg BW.

Hepatoprotective effect of Indonesian propolis from <italic>Apis mellifera</italic> in carbon tetrachloride (CCl4) induced liver injury in mice

Departement of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Hepatoprotective effect of Indonesian propolis from  in carbon tetrachloride (CCl) induced liver injury in mice

Propolis has been reported to have a broad spectrum of biological activities. The objective of this study was to investigate the hepatoprotective effect of...

Hepatoprotective effect of Indonesian propolis from Apis mellifera in carbon tetrachloride (CCl₄) induced liver injury in mice

Published Online: Dec 25, 2023

Page range: 189 - 193

Received: May 09, 2022

Accepted: Oct 25, 2023

DOI: https://doi.org/10.2478/cipms-2023-0033

Keywords
hepatoprotective, propolis, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, liver histology

© 2023 Dian Ayu Juwita et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Hepatoprotective effect of Indonesian propolis from Apis mellifera in carbon tetrachloride (CCl4) induced liver injury in mice

Published Online: Dec 25, 2023

Page range: 189 - 193

Received: May 09, 2022

Accepted: Oct 25, 2023

DOI: https://doi.org/10.2478/cipms-2023-0033

Keywordshepatoprotective, propolis, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, liver histology

© 2023 Dian Ayu Juwita et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Hepatoprotective effect of Indonesian propolis from Apis mellifera in carbon tetrachloride (CCl₄) induced liver injury in mice

Keywords
hepatoprotective, propolis, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, liver histology