<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>The aim of the study</bold>. To evaluate the effect of succinic acid derivatives on changes of mitochondrial function in rats under cerebral ischemia conditions.<bold>Materials and methods</bold>. In this work, the effect of succinic acid, ethylmethylhydroxypyridine succinate, and acetylaminosuccinic acid at doses of 50 mg/kg, 100 mg/kg, and 200 mg/kg (<italic>per os</italic>) on the change of the neuronal mitochondria function was studied. Cerebral ischemia was reproduced by <italic>the Tamura</italic> method. The following parameters were evaluated: changes in aerobic/anaerobic metabolism, mitochondrial membrane potential, the opening rate of the mitochondrial pore of transitional permeability and the activity of apoptotic systems.<bold>Results.</bold> During the study, it was found that the use of the test-compounds at doses of 100 mg/kg and 200 mg/kg contributed to an increase in ATP-generating activity, as well as the maximum respiration level and respiratory capacity, while accompanied by a decrease in the intensity of anaerobic metabolism reactions. Also, upon administration of the test succinic acid derivatives, an increase in the mitochondrial membrane potential and latent opening time of the mitochondrial pore transitional permeability were observed. Moreover, the activity of caspase-3 and apoptosis-inducing factor on groups treated by test objects at doses of 100 mg/kg and 200 mg/kg was significantly lower than that in untreated animals.<bold>Conclusion.</bold> The studied succinic acid derivatives contribute to the restoration of mitochondrial function in cerebral ischemia conditions, while the most effective dose can be considered to be 100 mg/kg.</abstract>

The aim of the study. To evaluate the effect of succinic acid derivatives on changes of mitochondrial function in rats under cerebral ischemia conditions.Materials and methods. In this work, the effect of succinic acid, ethylmethylhydroxypyridine succinate, and acetylaminosuccinic acid at doses of 50 mg/kg, 100 mg/kg, and 200 mg/kg (per os) on the change of the neuronal mitochondria function was studied. Cerebral ischemia was reproduced by the Tamura method. The following parameters were evaluated: changes in aerobic/anaerobic metabolism, mitochondrial membrane potential, the opening rate of the mitochondrial pore of transitional permeability and the activity of apoptotic systems.Results. During the study, it was found that the use of the test-compounds at doses of 100 mg/kg and 200 mg/kg contributed to an increase in ATP-generating activity, as well as the maximum respiration level and respiratory capacity, while accompanied by a decrease in the intensity of anaerobic metabolism reactions. Also, upon administration of the test succinic acid derivatives, an increase in the mitochondrial membrane potential and latent opening time of the mitochondrial pore transitional permeability were observed. Moreover, the activity of caspase-3 and apoptosis-inducing factor on groups treated by test objects at doses of 100 mg/kg and 200 mg/kg was significantly lower than that in untreated animals.Conclusion. The studied succinic acid derivatives contribute to the restoration of mitochondrial function in cerebral ischemia conditions, while the most effective dose can be considered to be 100 mg/kg.

Correction of mitochondrial dysfunction by succinic acid derivatives under experimental cerebral ischemia conditions

Department of Pharmacology with Course of Clinical Pharmacology

a Branch Volgograd State Medical University

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The aim of the study. To evaluate the effect of succinic acid derivatives on changes of mitochondrial function in rats under cerebral ischemia...

Correction of mitochondrial dysfunction by succinic acid derivatives under experimental cerebral ischemia conditions

Published Online: Mar 22, 2021

Page range: 42 - 48

Received: Feb 21, 2020

Accepted: Aug 14, 2020

DOI: https://doi.org/10.2478/cipms-2021-0008

Keywords
cerebral ischemia, mitochondrial dysfunction, succinates

© 2021 Dmitry I. Pozdnyakov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Correction of mitochondrial dysfunction by succinic acid derivatives under experimental cerebral ischemia conditions

Published Online: Mar 22, 2021

Page range: 42 - 48

Received: Feb 21, 2020

Accepted: Aug 14, 2020

DOI: https://doi.org/10.2478/cipms-2021-0008

Keywordscerebral ischemia, mitochondrial dysfunction, succinates

© 2021 Dmitry I. Pozdnyakov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
cerebral ischemia, mitochondrial dysfunction, succinates