<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> The study was conducted to detect Brucella sp. and Leptospira sp. in blood samples of dogs in Isfahan and Shahrekord province in Iran. A total of 94 blood samples were collected from dogs of different breed, age, sex, and dogs’ type (stray or nonstray). The samples were examined using conventional polymerase chain reaction (PCR). Fourteen (14.89%) dogs were positive for Brucella sp. and 18 (19.15%). dogs for Leptospira sp. There were no significant differences between the prevalence of the pathogens, provinces, sex, and age groups (P > 0.05). However, there was a statistically significant difference in prevalence of Brucella sp. and Leptospira sp. between stray and non-stray dogs (P &lt; 0.0001; χ2 = 30.3767). The study also demonstrated that PCR was successfully used for the first time in Iran for the detection of Brucella sp. and Leptospira sp. in blood samples of dogs. Therefore, we recommend the PCR as a supplementary method with other commonly recognised methods (e.g. serological methods) for the diagnosis of subclinical infections with the microorganisms. Strict measures for the control of stray dogs are also highly recommended.</p>
</abstract>

The study was conducted to detect Brucella sp. and Leptospira sp. in blood samples of dogs in Isfahan and Shahrekord province in Iran. A total of 94 blood samples were collected from dogs of different breed, age, sex, and dogs’ type (stray or nonstray). The samples were examined using conventional polymerase chain reaction (PCR). Fourteen (14.89%) dogs were positive for Brucella sp. and 18 (19.15%). dogs for Leptospira sp. There were no significant differences between the prevalence of the pathogens, provinces, sex, and age groups (P > 0.05). However, there was a statistically significant difference in prevalence of Brucella sp. and Leptospira sp. between stray and non-stray dogs (P < 0.0001; χ2 = 30.3767). The study also demonstrated that PCR was successfully used for the first time in Iran for the detection of Brucella sp. and Leptospira sp. in blood samples of dogs. Therefore, we recommend the PCR as a supplementary method with other commonly recognised methods (e.g. serological methods) for the diagnosis of subclinical infections with the microorganisms. Strict measures for the control of stray dogs are also highly recommended.

Detection of Brucella sp. and Leptospira sp. in dogs using conventional polymerase chain reaction

Young Researchers and Elite Club, Biotechnology Research Center, Faculty of Veterinary Medicine

Shahrekord Branch, Islamic Azad University

Faculty of Veterinary Medicine, Shahrekord Branch

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The study was conducted to detect Brucella sp. and Leptospira sp. in blood samples of dogs in Isfahan and Shahrekord province in Iran. A total of 94 blood...

Detection of Brucella sp. and Leptospira sp. in dogs using conventional polymerase chain reaction

Published Online: Dec 20, 2014

Page range: 527 - 531

Received: May 01, 2014

Accepted: Oct 14, 2014

DOI: https://doi.org/10.2478/bvip-2014-0081

Keywords
dogs, leptospirosis, brucellosis, PCR, epidemiology, Iran

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Detection of Brucella sp. and Leptospira sp. in dogs using conventional polymerase chain reaction

Published Online: Dec 20, 2014

Page range: 527 - 531

Received: May 01, 2014

Accepted: Oct 14, 2014

DOI: https://doi.org/10.2478/bvip-2014-0081

Keywordsdogs, leptospirosis, brucellosis, PCR, epidemiology, Iran

© 2014 National Veterinary Research Institute in Pulawy

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
dogs, leptospirosis, brucellosis, PCR, epidemiology, Iran